Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology

Protease is a versatile industrial enzymes that is applicable in various industries including the food sector. Eventhough most commercial proteases are from animal and microbial origin, they each carries certain limitations involving religious, ethical and dietary concerns. Therefore, the discovery...

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Main Authors:	Shuhaimen, Muhammad Shahrain, Omar, Muhammad Nor, Abdullah, Erna Normaya, Ahmad, Mohammad Norazmi
Format:	Conference or Workshop Item
Language:	English English
Published:	2017
Subjects:	S Agriculture (General)
Online Access:	http://irep.iium.edu.my/58306/ http://irep.iium.edu.my/58306/ http://irep.iium.edu.my/58306/1/Muhammad%20Shahrain%20Poster%20%20iCast2017.pdf http://irep.iium.edu.my/58306/19/58306_Icast.pdf

id	iium-58306
recordtype	eprints
spelling	iium-583062017-09-12T01:35:49Z http://irep.iium.edu.my/58306/ Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology Shuhaimen, Muhammad Shahrain Omar, Muhammad Nor Abdullah, Erna Normaya Ahmad, Mohammad Norazmi S Agriculture (General) Protease is a versatile industrial enzymes that is applicable in various industries including the food sector. Eventhough most commercial proteases are from animal and microbial origin, they each carries certain limitations involving religious, ethical and dietary concerns. Therefore, the discovery of proteases from plant as an alternative source should be given attention. In this study cashew (Anacardium occidentale) fruit, known to have dermatitic and anthelmintic properties, was chosen as source of protease, and the effect of the extraction process on its protease activity is evaluated. The extracted crude enzyme was optimized by using response surface methodology (RSM) using a central composite rotatable design (CCRD) with variables studied were pH (x1), CaCl2 (x2), mixing time (x3) and mass (x4). The quadratic model obtained from the results is significant (R2 = 0.9739). Based on the RSM model, the optimal extraction conditions were obtained at pH 6.42, 8.94 mM CaCl2, 5.62 min mixing time and 18.55 g sample mass that yields protease 6.251 unit/mL of crude extract. 2017-08-08 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/58306/1/Muhammad%20Shahrain%20Poster%20%20iCast2017.pdf application/pdf en http://irep.iium.edu.my/58306/19/58306_Icast.pdf Shuhaimen, Muhammad Shahrain and Omar, Muhammad Nor and Abdullah, Erna Normaya and Ahmad, Mohammad Norazmi (2017) Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology. In: 6th International Conference on Advancement in Science and Technology (iCAST 2017), 8th-9th August 2017, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/icast/?page_id=722
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	S Agriculture (General)
spellingShingle	S Agriculture (General) Shuhaimen, Muhammad Shahrain Omar, Muhammad Nor Abdullah, Erna Normaya Ahmad, Mohammad Norazmi Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology
description	Protease is a versatile industrial enzymes that is applicable in various industries including the food sector. Eventhough most commercial proteases are from animal and microbial origin, they each carries certain limitations involving religious, ethical and dietary concerns. Therefore, the discovery of proteases from plant as an alternative source should be given attention. In this study cashew (Anacardium occidentale) fruit, known to have dermatitic and anthelmintic properties, was chosen as source of protease, and the effect of the extraction process on its protease activity is evaluated. The extracted crude enzyme was optimized by using response surface methodology (RSM) using a central composite rotatable design (CCRD) with variables studied were pH (x1), CaCl2 (x2), mixing time (x3) and mass (x4). The quadratic model obtained from the results is significant (R2 = 0.9739). Based on the RSM model, the optimal extraction conditions were obtained at pH 6.42, 8.94 mM CaCl2, 5.62 min mixing time and 18.55 g sample mass that yields protease 6.251 unit/mL of crude extract.
format	Conference or Workshop Item
author	Shuhaimen, Muhammad Shahrain Omar, Muhammad Nor Abdullah, Erna Normaya Ahmad, Mohammad Norazmi
author_facet	Shuhaimen, Muhammad Shahrain Omar, Muhammad Nor Abdullah, Erna Normaya Ahmad, Mohammad Norazmi
author_sort	Shuhaimen, Muhammad Shahrain
title	Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology
title_short	Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology
title_full	Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology
title_fullStr	Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology
title_full_unstemmed	Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology
title_sort	extraction of protease from anacardium occidentale (cashew) fruit using response surface methodology
publishDate	2017
url	http://irep.iium.edu.my/58306/ http://irep.iium.edu.my/58306/ http://irep.iium.edu.my/58306/1/Muhammad%20Shahrain%20Poster%20%20iCast2017.pdf http://irep.iium.edu.my/58306/19/58306_Icast.pdf
first_indexed	2023-09-18T21:22:26Z
last_indexed	2023-09-18T21:22:26Z
_version_	1777411970692022272

Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology

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