Early effects of high cholesterol diet on the kidney of an animal model

between progressive kidney damage and hypercholesterolemia. Most studies focused on the impact of chronic high blood cholesterol levels on the kidney. Information on the early effect of hypercholesterolemia on the kidney is still lacking. The aim of this study was therefore to determine early eff...

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Main Authors:	Hamad Mohamed, Zenab, Hamad Alfarisi, Hamad Abdulsalam, Abdullah, Nor Zamzila, Harun, Norra, Muhammad, Naznin, Abdul Rahim, Roslina
Format:	Article
Language:	English
Published:	IMJM 2017
Subjects:	RA Public aspects of medicine RC Internal medicine
Online Access:	http://irep.iium.edu.my/58508/ http://irep.iium.edu.my/58508/ http://irep.iium.edu.my/58508/1/Abstract-Book-for-IMJM-final-v2-26.pdf

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recordtype	eprints
spelling	iium-585082018-02-03T20:02:54Z http://irep.iium.edu.my/58508/ Early effects of high cholesterol diet on the kidney of an animal model Hamad Mohamed, Zenab Hamad Alfarisi, Hamad Abdulsalam Abdullah, Nor Zamzila Harun, Norra Muhammad, Naznin Abdul Rahim, Roslina RA Public aspects of medicine RC Internal medicine between progressive kidney damage and hypercholesterolemia. Most studies focused on the impact of chronic high blood cholesterol levels on the kidney. Information on the early effect of hypercholesterolemia on the kidney is still lacking. The aim of this study was therefore to determine early effect of high cholesterol diet on the kidney in an animal model. Materials and method: Ten female Sprague-Dawley rats were divided into two groups. The control group was fed with commercial rat pellet while the high cholesterol diet (HCD) group was fed with 12% cholesterol diet and 0.3% cholic acid. Biochemical analysis (lipid profile and renal function) was performed at 48 hours, 7 days and 6 weeks of the experiment. The animals were sacrificed at 6 weeks and the kidneys were harvested for histological examination. Results: The HCD group rats had significantly higher levels of serum total cholesterol (at 7 days and 6 weeks). The HDL-cholesterol and triglyceride levels were however lower at 6 weeks. The mean serum creatinine level of the HCD group were increased after 48 hours and 7 days compared to the control group. Histological examination of the kidney tissue of the HCD group at 6 weeks revealed segmental mesangial hypercellularity and mesangial matrix expansion of the glomeruli. Conclusion: The 12% cholesterol diet induced dyslipidaemia in the animal model. It resulted in acute kidney injury based on the serum creatinine at 48 hours and 7 days. Kidney tissues examined at 6 weeks revealed changes confined to mesangial cells of the renal glomeruli. IMJM 2017-09-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58508/1/Abstract-Book-for-IMJM-final-v2-26.pdf Hamad Mohamed, Zenab and Hamad Alfarisi, Hamad Abdulsalam and Abdullah, Nor Zamzila and Harun, Norra and Muhammad, Naznin and Abdul Rahim, Roslina (2017) Early effects of high cholesterol diet on the kidney of an animal model. International Medical Journal Malaysia (IMJM), 16 (supp. 1). p. 20. http://iiumedic.net/imjm/v1/volume-16-supplementary-issue-no-1/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RA Public aspects of medicine RC Internal medicine
spellingShingle	RA Public aspects of medicine RC Internal medicine Hamad Mohamed, Zenab Hamad Alfarisi, Hamad Abdulsalam Abdullah, Nor Zamzila Harun, Norra Muhammad, Naznin Abdul Rahim, Roslina Early effects of high cholesterol diet on the kidney of an animal model
description	between progressive kidney damage and hypercholesterolemia. Most studies focused on the impact of chronic high blood cholesterol levels on the kidney. Information on the early effect of hypercholesterolemia on the kidney is still lacking. The aim of this study was therefore to determine early effect of high cholesterol diet on the kidney in an animal model. Materials and method: Ten female Sprague-Dawley rats were divided into two groups. The control group was fed with commercial rat pellet while the high cholesterol diet (HCD) group was fed with 12% cholesterol diet and 0.3% cholic acid. Biochemical analysis (lipid profile and renal function) was performed at 48 hours, 7 days and 6 weeks of the experiment. The animals were sacrificed at 6 weeks and the kidneys were harvested for histological examination. Results: The HCD group rats had significantly higher levels of serum total cholesterol (at 7 days and 6 weeks). The HDL-cholesterol and triglyceride levels were however lower at 6 weeks. The mean serum creatinine level of the HCD group were increased after 48 hours and 7 days compared to the control group. Histological examination of the kidney tissue of the HCD group at 6 weeks revealed segmental mesangial hypercellularity and mesangial matrix expansion of the glomeruli. Conclusion: The 12% cholesterol diet induced dyslipidaemia in the animal model. It resulted in acute kidney injury based on the serum creatinine at 48 hours and 7 days. Kidney tissues examined at 6 weeks revealed changes confined to mesangial cells of the renal glomeruli.
format	Article
author	Hamad Mohamed, Zenab Hamad Alfarisi, Hamad Abdulsalam Abdullah, Nor Zamzila Harun, Norra Muhammad, Naznin Abdul Rahim, Roslina
author_facet	Hamad Mohamed, Zenab Hamad Alfarisi, Hamad Abdulsalam Abdullah, Nor Zamzila Harun, Norra Muhammad, Naznin Abdul Rahim, Roslina
author_sort	Hamad Mohamed, Zenab
title	Early effects of high cholesterol diet on the kidney of an animal model
title_short	Early effects of high cholesterol diet on the kidney of an animal model
title_full	Early effects of high cholesterol diet on the kidney of an animal model
title_fullStr	Early effects of high cholesterol diet on the kidney of an animal model
title_full_unstemmed	Early effects of high cholesterol diet on the kidney of an animal model
title_sort	early effects of high cholesterol diet on the kidney of an animal model
publisher	IMJM
publishDate	2017
url	http://irep.iium.edu.my/58508/ http://irep.iium.edu.my/58508/ http://irep.iium.edu.my/58508/1/Abstract-Book-for-IMJM-final-v2-26.pdf
first_indexed	2023-09-18T21:22:44Z
last_indexed	2023-09-18T21:22:44Z
_version_	1777411989805465600

Early effects of high cholesterol diet on the kidney of an animal model

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