Development, validation and pharmacokinetic application of a simple and robust RP-HPLC method for quantitation of raloxifene in rat plasma
A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a mobile phas...
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| Format: | Article |
| Language: | English English |
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Colegio de Farmaceuticos de la Provincia de Buenos Aires
2017
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| Online Access: | http://irep.iium.edu.my/58589/ http://irep.iium.edu.my/58589/ http://irep.iium.edu.my/58589/1/Latin%20Journal%20LAJOP_36_9_1_35.pdf http://irep.iium.edu.my/58589/7/58589_Development%2C%20validation_scopus.pdf |
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iium-585892018-04-18T03:19:14Z http://irep.iium.edu.my/58589/ Development, validation and pharmacokinetic application of a simple and robust RP-HPLC method for quantitation of raloxifene in rat plasma Syed Jaffri, Syed Mahmood Sengupta, Pinaki Ranjan Mandal, Uttam Kumar Chatterjee, Bappaditya D. Bakhtiar, M. Taher RM300 Drugs and their action A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a mobile phase consisted of phosphate buffer and acetonitrile (66:34 %, v/v). The method was validated for specificity, selectivity, sensitivity, linearity, accuracy, precision, recovery and stability parameters. A linear response (R2 value 0.9991) was found over the calibration range of 50 to 500 ng/mL. The accuracy for intra and inter day run varied between 86.73 to 102.30 % and 91.11 to 95.56%, respectively. The corresponding precision (% CV) were within 0.82 to 9.43% and 6.23 to 8.33%. The method was also found to be specific and stable. The applicability of the method was established through a single dose oral pharmacokinetic study of raloxifene in rat. Colegio de Farmaceuticos de la Provincia de Buenos Aires 2017-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58589/1/Latin%20Journal%20LAJOP_36_9_1_35.pdf application/pdf en http://irep.iium.edu.my/58589/7/58589_Development%2C%20validation_scopus.pdf Syed Jaffri, Syed Mahmood and Sengupta, Pinaki Ranjan and Mandal, Uttam Kumar and Chatterjee, Bappaditya D. and Bakhtiar, M. Taher (2017) Development, validation and pharmacokinetic application of a simple and robust RP-HPLC method for quantitation of raloxifene in rat plasma. Latin American Journal of Pharmacy, 36 (9). pp. 1901-1907. ISSN 0326 2383 http://www.latamjpharm.org/ |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
RM300 Drugs and their action |
| spellingShingle |
RM300 Drugs and their action Syed Jaffri, Syed Mahmood Sengupta, Pinaki Ranjan Mandal, Uttam Kumar Chatterjee, Bappaditya D. Bakhtiar, M. Taher Development, validation and pharmacokinetic application of a simple and robust RP-HPLC method for quantitation of raloxifene in rat plasma |
| description |
A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a mobile phase consisted of phosphate buffer and acetonitrile (66:34 %, v/v). The method was validated for specificity, selectivity, sensitivity, linearity, accuracy, precision, recovery and stability parameters. A linear response (R2 value 0.9991) was found over the calibration range of 50 to 500 ng/mL. The accuracy for intra
and inter day run varied between 86.73 to 102.30 % and 91.11 to 95.56%, respectively. The corresponding
precision (% CV) were within 0.82 to 9.43% and 6.23 to 8.33%. The method was also found to be specific
and stable. The applicability of the method was established through a single dose oral pharmacokinetic
study of raloxifene in rat. |
| format |
Article |
| author |
Syed Jaffri, Syed Mahmood Sengupta, Pinaki Ranjan Mandal, Uttam Kumar Chatterjee, Bappaditya D. Bakhtiar, M. Taher |
| author_facet |
Syed Jaffri, Syed Mahmood Sengupta, Pinaki Ranjan Mandal, Uttam Kumar Chatterjee, Bappaditya D. Bakhtiar, M. Taher |
| author_sort |
Syed Jaffri, Syed Mahmood |
| title |
Development, validation and pharmacokinetic application
of a simple and robust RP-HPLC method for quantitation
of raloxifene in rat plasma |
| title_short |
Development, validation and pharmacokinetic application
of a simple and robust RP-HPLC method for quantitation
of raloxifene in rat plasma |
| title_full |
Development, validation and pharmacokinetic application
of a simple and robust RP-HPLC method for quantitation
of raloxifene in rat plasma |
| title_fullStr |
Development, validation and pharmacokinetic application
of a simple and robust RP-HPLC method for quantitation
of raloxifene in rat plasma |
| title_full_unstemmed |
Development, validation and pharmacokinetic application
of a simple and robust RP-HPLC method for quantitation
of raloxifene in rat plasma |
| title_sort |
development, validation and pharmacokinetic application
of a simple and robust rp-hplc method for quantitation
of raloxifene in rat plasma |
| publisher |
Colegio de Farmaceuticos de la Provincia de Buenos Aires |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/58589/ http://irep.iium.edu.my/58589/ http://irep.iium.edu.my/58589/1/Latin%20Journal%20LAJOP_36_9_1_35.pdf http://irep.iium.edu.my/58589/7/58589_Development%2C%20validation_scopus.pdf |
| first_indexed |
2023-09-18T21:22:53Z |
| last_indexed |
2023-09-18T21:22:53Z |
| _version_ |
1777411999234260992 |