Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review

Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review

Nowadays, the use of cell-free fetal DNA (cfDNA) is a promising tool in clinical practice as a potential non-invasive prenatal testing (NIPT) method since it was discovered in early 1970s. The fetal DNA is approximately ~10% in a mixture of maternal DNA from maternal plasma and this amount will incr...

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Main Authors: Abdul Ghafar, Nurul Fatehah, Zainuddin, Norafiza
Format: Conference or Workshop Item
Language:English
Published: IIUM 2017
Subjects:
QH426 Genetics
RG Gynecology and obstetrics
Online Access:http://irep.iium.edu.my/58748/
http://irep.iium.edu.my/58748/
http://irep.iium.edu.my/58748/1/58748_Non-Invasive%20Prenatal.pdf
id iium-58748
recordtype eprints
spelling iium-587482017-11-03T03:07:19Z http://irep.iium.edu.my/58748/ Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review Abdul Ghafar, Nurul Fatehah Zainuddin, Norafiza QH426 Genetics RG Gynecology and obstetrics Nowadays, the use of cell-free fetal DNA (cfDNA) is a promising tool in clinical practice as a potential non-invasive prenatal testing (NIPT) method since it was discovered in early 1970s. The fetal DNA is approximately ~10% in a mixture of maternal DNA from maternal plasma and this amount will increase as gestation period increases. In recent years, the development of robust molecular analysis of fetal DNA namely via RT-PCR, next generation sequencing (NGS), digital PCR and massively parallel sequencing (MPS) helps the implementation of NIPT especially in fetal chromosomal aneuploidy detection. Thus, these analyses provide the alternative to the conventional invasive prenatal testing such as amniocentesis and chorionic villus sampling (CVS). The common fetal aneuploidy is trisomy 21 (T21) which is caused by an extra copy of all or part of chromosome 21 and known to be validated by amniocentesis approach as the gold standard method. Currently, the epigenetic detection of trisomy 21 had been introduced as a new non-invasive method that investigates the association between DNA methylation and gene expression in T21 fetal DNA. This review briefly summarizes the NIPT and invasive prenatal testing of fetal aneuploidy and recent molecular analysis study by using cfDNA from maternal plasma. IIUM 2017-09-16 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/58748/1/58748_Non-Invasive%20Prenatal.pdf Abdul Ghafar, Nurul Fatehah and Zainuddin, Norafiza (2017) Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review. In: KAHS Research Week 2016, Kulliyyah of Allied Health Sciences. http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/79
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QH426 Genetics
RG Gynecology and obstetrics
spellingShingle QH426 Genetics
RG Gynecology and obstetrics
Abdul Ghafar, Nurul Fatehah
Zainuddin, Norafiza
Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review
description Nowadays, the use of cell-free fetal DNA (cfDNA) is a promising tool in clinical practice as a potential non-invasive prenatal testing (NIPT) method since it was discovered in early 1970s. The fetal DNA is approximately ~10% in a mixture of maternal DNA from maternal plasma and this amount will increase as gestation period increases. In recent years, the development of robust molecular analysis of fetal DNA namely via RT-PCR, next generation sequencing (NGS), digital PCR and massively parallel sequencing (MPS) helps the implementation of NIPT especially in fetal chromosomal aneuploidy detection. Thus, these analyses provide the alternative to the conventional invasive prenatal testing such as amniocentesis and chorionic villus sampling (CVS). The common fetal aneuploidy is trisomy 21 (T21) which is caused by an extra copy of all or part of chromosome 21 and known to be validated by amniocentesis approach as the gold standard method. Currently, the epigenetic detection of trisomy 21 had been introduced as a new non-invasive method that investigates the association between DNA methylation and gene expression in T21 fetal DNA. This review briefly summarizes the NIPT and invasive prenatal testing of fetal aneuploidy and recent molecular analysis study by using cfDNA from maternal plasma.
format Conference or Workshop Item
author Abdul Ghafar, Nurul Fatehah
Zainuddin, Norafiza
author_facet Abdul Ghafar, Nurul Fatehah
Zainuddin, Norafiza
author_sort Abdul Ghafar, Nurul Fatehah
title Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review
title_short Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review
title_full Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review
title_fullStr Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review
title_full_unstemmed Non-invasive prenatal testing using cell-free fetal DNA from maternal plasma: a review
title_sort non-invasive prenatal testing using cell-free fetal dna from maternal plasma: a review
publisher IIUM
publishDate 2017
url http://irep.iium.edu.my/58748/
http://irep.iium.edu.my/58748/
http://irep.iium.edu.my/58748/1/58748_Non-Invasive%20Prenatal.pdf
first_indexed 2023-09-18T21:23:07Z
last_indexed 2023-09-18T21:23:07Z
_version_ 1777412013837778944

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