The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure

Studies have reported that exposure to cyclophosphamide (CPA) for cancer treatment result in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sperm defects. Our goal is to assess the potential effe...

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Main Authors:	Abdul Samat, Nadia Hanis, Abdul Rahman, Suzanah, Ahmad, Nur Amalina, Abdullah, 'Afif Raihan, Mohamad Zahri, Syazana
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/58769/ http://irep.iium.edu.my/58769/1/ICT%20BIO%20Asia%202016_Suzanah.pdf http://irep.iium.edu.my/58769/3/ICT%20Bio%202016%20Poster.pdf

id	iium-58769
recordtype	eprints
spelling	iium-587692017-10-25T07:39:08Z http://irep.iium.edu.my/58769/ The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Ahmad, Nur Amalina Abdullah, 'Afif Raihan Mohamad Zahri, Syazana RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM300 Drugs and their action Studies have reported that exposure to cyclophosphamide (CPA) for cancer treatment result in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sperm defects. Our goal is to assess the potential effects of Nigella sativa extract (NSE) and thymoquinone (TQ) on sperm and embryo quality following fertlization from sperm produced from germ cells which have been exposed to the damaging alkylating effects of CPA. Thirty male ICR mice were divided into six groups; (I) Group 1: Vehicle-treated control (normal saline), (II)Group 2: CPA-only, (III)Group 3: TQ-only, (IV)Group 4: NSE-only, (V) Group 5: CPA followed by TQ and (VI)Group 6: CPA followed by NSE. Treatment was given to the mice based on the grouping for 32 days where the dosage for CPA is 200mg/kg and for NSE and TQ at 10mg/kg. Groups (V) and (VI) were treated with CPA followed by NSE and TQ after six hours. Animals were sacrificed at 33 days by cervical dislocation and sperm from caudal epidydymis were taken for analysis and in vitro fertilization (IVF) with eggs from untreated female. Fertilization rates and embryo development were assessed and monitored for 5 days. TQ and NSE supplementation to CPA-exposed male mice have no significant effect (p>0.05) on the total number of sperm if compared to CPA-only exposed mice. NSE and TQ supplementation has been shown to have significant effect (p<0.05) on the percentage of motile sperm as well as the number of abnormal sperm. There are four types of abnormalities of the sperm were found which includes folded sperm, amorphous, banana-like and lastly the head lack of usual hook. Finally, the embryo quality based on embryo scoring by Baczkowski (2004), shows a significant improvement by the supplementation of TQ and NSE to CPA-exposed male mice (p<0.05). Overall, both NSE and TQ have indicated chemopreventive potential against the cytotoxicity of cyclophosphamide on the reproductive capacity and fertility. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/58769/1/ICT%20BIO%20Asia%202016_Suzanah.pdf application/pdf en http://irep.iium.edu.my/58769/3/ICT%20Bio%202016%20Poster.pdf Abdul Samat, Nadia Hanis and Abdul Rahman, Suzanah and Ahmad, Nur Amalina and Abdullah, 'Afif Raihan and Mohamad Zahri, Syazana (2016) The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure. In: ICT/BIO ASIA 2016 Regional Workshop Programme, 30th-31st May 2016, Institut Pengurusan dan Pemantauan Penyelidikan (IPPP) –University of Malaya. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM300 Drugs and their action
spellingShingle	RG Gynecology and obstetrics RM Therapeutics. Pharmacology RM300 Drugs and their action Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Ahmad, Nur Amalina Abdullah, 'Afif Raihan Mohamad Zahri, Syazana The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
description	Studies have reported that exposure to cyclophosphamide (CPA) for cancer treatment result in adverse reproductive consequences. Over-production of reactive oxygen species causes oxidative stress thus affecting the DNA in male germ cell inducing sperm defects. Our goal is to assess the potential effects of Nigella sativa extract (NSE) and thymoquinone (TQ) on sperm and embryo quality following fertlization from sperm produced from germ cells which have been exposed to the damaging alkylating effects of CPA. Thirty male ICR mice were divided into six groups; (I) Group 1: Vehicle-treated control (normal saline), (II)Group 2: CPA-only, (III)Group 3: TQ-only, (IV)Group 4: NSE-only, (V) Group 5: CPA followed by TQ and (VI)Group 6: CPA followed by NSE. Treatment was given to the mice based on the grouping for 32 days where the dosage for CPA is 200mg/kg and for NSE and TQ at 10mg/kg. Groups (V) and (VI) were treated with CPA followed by NSE and TQ after six hours. Animals were sacrificed at 33 days by cervical dislocation and sperm from caudal epidydymis were taken for analysis and in vitro fertilization (IVF) with eggs from untreated female. Fertilization rates and embryo development were assessed and monitored for 5 days. TQ and NSE supplementation to CPA-exposed male mice have no significant effect (p>0.05) on the total number of sperm if compared to CPA-only exposed mice. NSE and TQ supplementation has been shown to have significant effect (p<0.05) on the percentage of motile sperm as well as the number of abnormal sperm. There are four types of abnormalities of the sperm were found which includes folded sperm, amorphous, banana-like and lastly the head lack of usual hook. Finally, the embryo quality based on embryo scoring by Baczkowski (2004), shows a significant improvement by the supplementation of TQ and NSE to CPA-exposed male mice (p<0.05). Overall, both NSE and TQ have indicated chemopreventive potential against the cytotoxicity of cyclophosphamide on the reproductive capacity and fertility.
format	Conference or Workshop Item
author	Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Ahmad, Nur Amalina Abdullah, 'Afif Raihan Mohamad Zahri, Syazana
author_facet	Abdul Samat, Nadia Hanis Abdul Rahman, Suzanah Ahmad, Nur Amalina Abdullah, 'Afif Raihan Mohamad Zahri, Syazana
author_sort	Abdul Samat, Nadia Hanis
title	The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_short	The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_full	The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_fullStr	The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_full_unstemmed	The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
title_sort	potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure
publishDate	2016
url	http://irep.iium.edu.my/58769/ http://irep.iium.edu.my/58769/1/ICT%20BIO%20Asia%202016_Suzanah.pdf http://irep.iium.edu.my/58769/3/ICT%20Bio%202016%20Poster.pdf
first_indexed	2023-09-18T21:23:08Z
last_indexed	2023-09-18T21:23:08Z
_version_	1777412015642378240

The potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure

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