Imbalanced expression of polycistronic miRNA in acute myeloid leukemia
miR-1 and miR-133 are clustered on the same chromosomal loci and are transcribed together as a single transcript that is positively regulated by ecotropic virus integration site-1 (EVI1). Previously, we described how miR-133 has anti-tumorigenic potential through repression of EVI1 expression. It...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer International Publishing AG
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/58944/ http://irep.iium.edu.my/58944/ http://irep.iium.edu.my/58944/ http://irep.iium.edu.my/58944/1/10.1007%40s12185-017-2314-1.pdf http://irep.iium.edu.my/58944/7/Imbalanced%20expression%20of%20polycistronic%20miRNA%20in%20acute%20myeloid%20leukemia.pdf |
| Summary: | miR-1 and miR-133 are clustered on the same chromosomal loci and are transcribed together as a single transcript that is positively regulated by ecotropic virus integration site-1 (EVI1). Previously, we described how
miR-133 has anti-tumorigenic potential through repression
of EVI1 expression. It has also been reported that miR-1 is oncogenic in the case of acute myeloid leukemia (AML).
Here, we show that expression of miR-1 and miR-133, which
have distinct functions, is differentially regulated between AML cell lines. Interestingly, the expression of miR-1 and EVI1, which binds to the promoter of the miR-1/miR-133 cluster, is correlative. The expression levels of TDP-43, an RNA-binding protein that has been reported to increase the expression, but inhibits the activity, of miR-1, were not correlated with expression levels of miR-1 in AML cells. Taken together, our observations raise the possibility that the balance of polycistronic miRNAs is regulated post-transcriptionally in a hierarchical manner possibly involving EVI1,suggesting that the deregulation of this balance may play some role in AML cells with high EVI1 expression. |
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