Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses

Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of Southeast Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counti...

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Main Author: Nordin, Mohd Shukor
Format: Article
Language:English
English
English
English
English
Published: INFORMA UK LIMITED 2018
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Online Access:http://irep.iium.edu.my/59387/
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http://irep.iium.edu.my/59387/19/59387_Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L_SCOPUS.pdf
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spelling iium-593872019-01-24T02:35:19Z http://irep.iium.edu.my/59387/ Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses Nordin, Mohd Shukor SB Plant culture Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of Southeast Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counting, flow cytometry and k-mer analyses. Chromosome count analysis revealed that the chromosome number of G. mangostana varied from 74-110. The high number observed could be due to the occurrence of mutation and aneuploidy in G. mangostana. Using flow cytometry with Glycine max cv. Polanka (2C=2.5pg) used as standard, G. mangostana genome size was found to be 2C=6.00±0.17 pg. Meanwhile, genome survey of G. mangostana was performed using Illumina HiSeq 2000 DNA sequencing, k-mer analysis revealed that the genome size of G. mangostana was approximately 5.92 Gbp, or approximately 6.05 pg (1 pg DNA=0.9780x109 bp). Based on the flow cytometry and genome survey, the study concludes that the genome size of G. mangostana to be between 6.00 to 6.05 pg. INFORMA UK LIMITED 2018-01-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59387/1/AcceptedAuthorPublishingAgreement%20%281%29.pdf application/pdf en http://irep.iium.edu.my/59387/2/Manusript-1A-TCAR-2017-0022R3.doc application/pdf en http://irep.iium.edu.my/59387/9/proof%20of%20acceptance.pdf application/pdf en http://irep.iium.edu.my/59387/19/59387_Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/59387/25/59387%20Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L.%20via%20cytogenetics%2C%20flow%20cytometry%20and%20k-mer%20analyses_wos.pdf Nordin, Mohd Shukor (2018) Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses. Caryologia TCAR, 71 (1). pp. 35-44. ISSN 2165-5391
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
English
English
topic SB Plant culture
spellingShingle SB Plant culture
Nordin, Mohd Shukor
Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
description Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of Southeast Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counting, flow cytometry and k-mer analyses. Chromosome count analysis revealed that the chromosome number of G. mangostana varied from 74-110. The high number observed could be due to the occurrence of mutation and aneuploidy in G. mangostana. Using flow cytometry with Glycine max cv. Polanka (2C=2.5pg) used as standard, G. mangostana genome size was found to be 2C=6.00±0.17 pg. Meanwhile, genome survey of G. mangostana was performed using Illumina HiSeq 2000 DNA sequencing, k-mer analysis revealed that the genome size of G. mangostana was approximately 5.92 Gbp, or approximately 6.05 pg (1 pg DNA=0.9780x109 bp). Based on the flow cytometry and genome survey, the study concludes that the genome size of G. mangostana to be between 6.00 to 6.05 pg.
format Article
author Nordin, Mohd Shukor
author_facet Nordin, Mohd Shukor
author_sort Nordin, Mohd Shukor
title Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
title_short Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
title_full Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
title_fullStr Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
title_full_unstemmed Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
title_sort determination of the chromosome number and genome size of garcinia mangostana l. via cytogenetics, flow cytometry and k-mer analyses
publisher INFORMA UK LIMITED
publishDate 2018
url http://irep.iium.edu.my/59387/
http://irep.iium.edu.my/59387/1/AcceptedAuthorPublishingAgreement%20%281%29.pdf
http://irep.iium.edu.my/59387/2/Manusript-1A-TCAR-2017-0022R3.doc
http://irep.iium.edu.my/59387/9/proof%20of%20acceptance.pdf
http://irep.iium.edu.my/59387/19/59387_Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L_SCOPUS.pdf
http://irep.iium.edu.my/59387/25/59387%20Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L.%20via%20cytogenetics%2C%20flow%20cytometry%20and%20k-mer%20analyses_wos.pdf
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