Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses
Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of Southeast Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counti...
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iium-59387 |
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iium-593872019-01-24T02:35:19Z http://irep.iium.edu.my/59387/ Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses Nordin, Mohd Shukor SB Plant culture Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of Southeast Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counting, flow cytometry and k-mer analyses. Chromosome count analysis revealed that the chromosome number of G. mangostana varied from 74-110. The high number observed could be due to the occurrence of mutation and aneuploidy in G. mangostana. Using flow cytometry with Glycine max cv. Polanka (2C=2.5pg) used as standard, G. mangostana genome size was found to be 2C=6.00±0.17 pg. Meanwhile, genome survey of G. mangostana was performed using Illumina HiSeq 2000 DNA sequencing, k-mer analysis revealed that the genome size of G. mangostana was approximately 5.92 Gbp, or approximately 6.05 pg (1 pg DNA=0.9780x109 bp). Based on the flow cytometry and genome survey, the study concludes that the genome size of G. mangostana to be between 6.00 to 6.05 pg. INFORMA UK LIMITED 2018-01-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59387/1/AcceptedAuthorPublishingAgreement%20%281%29.pdf application/pdf en http://irep.iium.edu.my/59387/2/Manusript-1A-TCAR-2017-0022R3.doc application/pdf en http://irep.iium.edu.my/59387/9/proof%20of%20acceptance.pdf application/pdf en http://irep.iium.edu.my/59387/19/59387_Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/59387/25/59387%20Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L.%20via%20cytogenetics%2C%20flow%20cytometry%20and%20k-mer%20analyses_wos.pdf Nordin, Mohd Shukor (2018) Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses. Caryologia TCAR, 71 (1). pp. 35-44. ISSN 2165-5391 |
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Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
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IIUM Repository |
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Online Access |
| language |
English English English English English |
| topic |
SB Plant culture |
| spellingShingle |
SB Plant culture Nordin, Mohd Shukor Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| description |
Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of Southeast Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counting, flow cytometry and k-mer analyses. Chromosome count analysis revealed that the chromosome number of G. mangostana varied from 74-110. The high number observed could be due to the occurrence of mutation and aneuploidy in G. mangostana. Using flow cytometry with Glycine max cv. Polanka (2C=2.5pg) used as standard, G. mangostana genome size was found to be 2C=6.00±0.17 pg. Meanwhile, genome survey of G. mangostana was performed using Illumina HiSeq 2000 DNA sequencing, k-mer analysis revealed that the genome size of G. mangostana was approximately 5.92 Gbp, or approximately 6.05 pg (1 pg DNA=0.9780x109 bp). Based on the flow cytometry and genome survey, the study concludes that the genome size of G. mangostana to be between 6.00 to 6.05 pg. |
| format |
Article |
| author |
Nordin, Mohd Shukor |
| author_facet |
Nordin, Mohd Shukor |
| author_sort |
Nordin, Mohd Shukor |
| title |
Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_short |
Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_full |
Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_fullStr |
Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_full_unstemmed |
Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses |
| title_sort |
determination of the chromosome number and genome size of garcinia mangostana l. via cytogenetics, flow cytometry and k-mer analyses |
| publisher |
INFORMA UK LIMITED |
| publishDate |
2018 |
| url |
http://irep.iium.edu.my/59387/ http://irep.iium.edu.my/59387/1/AcceptedAuthorPublishingAgreement%20%281%29.pdf http://irep.iium.edu.my/59387/2/Manusript-1A-TCAR-2017-0022R3.doc http://irep.iium.edu.my/59387/9/proof%20of%20acceptance.pdf http://irep.iium.edu.my/59387/19/59387_Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L_SCOPUS.pdf http://irep.iium.edu.my/59387/25/59387%20Determination%20of%20the%20chromosome%20number%20and%20genome%20size%20of%20Garcinia%20mangostana%20L.%20via%20cytogenetics%2C%20flow%20cytometry%20and%20k-mer%20analyses_wos.pdf |
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2023-09-18T21:24:09Z |
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2023-09-18T21:24:09Z |
| _version_ |
1777412079113732096 |