MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system

Multiple-emulsion, solvent-evaporation method is employed to synthesise MICROSPHERiiUM. A biodegradable co-polymer, poly(L-lactic-co-glycolic acid) (PLGA), is used as the matrix to form the microspheres. Briefly, an appropriate amount of PLGA is dissolved in dichloromethane to form the primary emul...

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Main Authors:	Mohamed, Farahidah, Doolanea, Abd Almonem, Harun Ismail, Ahmad Fahmi
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2011
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/5939/ http://irep.iium.edu.my/5939/1/Content_Page_IIUM_Research.pdf

id	iium-5939
recordtype	eprints
spelling	iium-59392019-09-05T03:01:48Z http://irep.iium.edu.my/5939/ MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system Mohamed, Farahidah Doolanea, Abd Almonem Harun Ismail, Ahmad Fahmi RS Pharmacy and materia medica Multiple-emulsion, solvent-evaporation method is employed to synthesise MICROSPHERiiUM. A biodegradable co-polymer, poly(L-lactic-co-glycolic acid) (PLGA), is used as the matrix to form the microspheres. Briefly, an appropriate amount of PLGA is dissolved in dichloromethane to form the primary emulsion. This phase is then homogenised with on aqueous phase, containing surfactant and a model drug (e.g. plasmid DNA or small molecules drug) for a certain duration and at an appropriate speed. The resultant waterin- oil-in-water (w/o/w) emulsion is then dispersed in a bigger volume of aqueous stabiliser. Then the mixture is transferred to a continuously stirred hardening tank containing the same stabiliser. Stirring is continued for a certain duration to allow complete evaporation of the solvent. The hardened MICROSPHER-iiUM isharvested by means of centrifugation and washing with distilled before it was freeze-dried. Characterisation of the MICROSPHERiiUM is conducted to investigate its surface morphology, size distribution, encapsulation efficiency and in-vitro release profile. Different protocols are adopted depending on the types of the madel drug to analyse the model drug. This MICROSPHER-iium has demonstrated robustness in encapsulating different types of agents with substantial encapsulation efficiency. The controlled-release profile is also achievable due to the inherent degradation rate of the co-polymers, PLGA, of which the rate and duration are dependent on its molecular weight. The colloidal size of this delivery system and the release of drug that can be controlled are envisaged to enhance the quality of therapy in chronic diseases as it can improve patient compliance towards drug regiment owing to reduction in frequency of dosing and reduction in side effects. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/5939/1/Content_Page_IIUM_Research.pdf Mohamed, Farahidah and Doolanea, Abd Almonem and Harun Ismail, Ahmad Fahmi (2011) MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system. In: IIUM Research, Invention & Innovation Exhibition 2011. IIUM Press, Kuala Lumpur, p. 183. ISBN 978-983-3124-14-9
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohamed, Farahidah Doolanea, Abd Almonem Harun Ismail, Ahmad Fahmi MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system
description	Multiple-emulsion, solvent-evaporation method is employed to synthesise MICROSPHERiiUM. A biodegradable co-polymer, poly(L-lactic-co-glycolic acid) (PLGA), is used as the matrix to form the microspheres. Briefly, an appropriate amount of PLGA is dissolved in dichloromethane to form the primary emulsion. This phase is then homogenised with on aqueous phase, containing surfactant and a model drug (e.g. plasmid DNA or small molecules drug) for a certain duration and at an appropriate speed. The resultant waterin- oil-in-water (w/o/w) emulsion is then dispersed in a bigger volume of aqueous stabiliser. Then the mixture is transferred to a continuously stirred hardening tank containing the same stabiliser. Stirring is continued for a certain duration to allow complete evaporation of the solvent. The hardened MICROSPHER-iiUM isharvested by means of centrifugation and washing with distilled before it was freeze-dried. Characterisation of the MICROSPHERiiUM is conducted to investigate its surface morphology, size distribution, encapsulation efficiency and in-vitro release profile. Different protocols are adopted depending on the types of the madel drug to analyse the model drug. This MICROSPHER-iium has demonstrated robustness in encapsulating different types of agents with substantial encapsulation efficiency. The controlled-release profile is also achievable due to the inherent degradation rate of the co-polymers, PLGA, of which the rate and duration are dependent on its molecular weight. The colloidal size of this delivery system and the release of drug that can be controlled are envisaged to enhance the quality of therapy in chronic diseases as it can improve patient compliance towards drug regiment owing to reduction in frequency of dosing and reduction in side effects.
format	Book Chapter
author	Mohamed, Farahidah Doolanea, Abd Almonem Harun Ismail, Ahmad Fahmi
author_facet	Mohamed, Farahidah Doolanea, Abd Almonem Harun Ismail, Ahmad Fahmi
author_sort	Mohamed, Farahidah
title	MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system
title_short	MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system
title_full	MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system
title_fullStr	MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system
title_full_unstemmed	MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system
title_sort	microspher-iium, a novel, controlled-released drug/gene delivery system
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/5939/ http://irep.iium.edu.my/5939/1/Content_Page_IIUM_Research.pdf
first_indexed	2023-09-18T20:14:45Z
last_indexed	2023-09-18T20:14:45Z
_version_	1777407713050886144

MIcrospher-iiUM, a novel, controlled-released drug/gene delivery system

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