Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor

The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO...

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Main Authors:	Mel, Maizirwan, Arifin, Mohd Azmir, Sohif, Hajar Naemah, Syed Hasan, Sharifah
Format:	Article
Language:	English
Published:	Malaysian Medical Associations 2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5943/ http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf

id	iium-5943
recordtype	eprints
spelling	iium-59432011-11-29T07:31:35Z http://irep.iium.edu.my/5943/ Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah TP248.13 Biotechnology The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed, microcarrier concentration, and pO2 level are applied. Malaysian Medical Associations 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf Mel, Maizirwan and Arifin, Mohd Azmir and Sohif, Hajar Naemah and Syed Hasan, Sharifah (2010) Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor. Medical Journal of Malaysia, 65 . pp. 19-21. ISSN 0300-5283
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
description	The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed, microcarrier concentration, and pO2 level are applied.
format	Article
author	Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah
author_facet	Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah
author_sort	Mel, Maizirwan
title	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_short	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_full	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_fullStr	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_full_unstemmed	Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
title_sort	optimization of process conditions for high cell density proliferation of df-1 cells in bioreactor
publisher	Malaysian Medical Associations
publishDate	2010
url	http://irep.iium.edu.my/5943/ http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf
first_indexed	2023-09-18T20:14:46Z
last_indexed	2023-09-18T20:14:46Z
_version_	1777407713501773824

Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor

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