An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response

Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius) by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield, Response Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopted...

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Main Authors:	Yusof, Faridah, Faruck, Mohammad Omer, Chowdhury, Silvia
Format:	Article
Language:	English English
Published:	Faculty of Food Science & Technology, Universiti Pertanian Malaysia 2017
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/59630/ http://irep.iium.edu.my/59630/ http://irep.iium.edu.my/59630/1/59630_An%20approach%20to%20optimize%20process.pdf http://irep.iium.edu.my/59630/7/59630_An%20approach%20to%20optimize%20process%20parameter_scopus.pdf

id	iium-59630
recordtype	eprints
spelling	iium-596302018-06-25T04:01:36Z http://irep.iium.edu.my/59630/ An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response Yusof, Faridah Faruck, Mohammad Omer Chowdhury, Silvia TP155 Chemical engineering TP248.13 Biotechnology Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius) by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield, Response Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopted to optimize the extraction process parameters. The effect of independent parameters, namely, the homogenization temperature (°C), homogenization time (min) and solid (g) to the solvent (ml) ratio of the extraction process on the fungal growth was studied. The extracted samples obtained by conducting runs accorded by the experimental design showed varying degree of antifungal activity against Aspergillus niger, the selected fungal strain, as assayed by the ‘‘Poisoned agar technique’. The investigation showed that the optimum values of the extraction parameters for the maximum antifungal peptides were 5 minutes homogenization time, 4°C homogenization temperature and 3.5:1 solid to solvent ratio. This study reports the development of an extraction process that allows careful recovery of antifungal peptides from insect larvae. In the validation of the experimental model, the error between the actual value and the predicted value was determined to be 3.57%. Faculty of Food Science & Technology, Universiti Pertanian Malaysia 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59630/1/59630_An%20approach%20to%20optimize%20process.pdf application/pdf en http://irep.iium.edu.my/59630/7/59630_An%20approach%20to%20optimize%20process%20parameter_scopus.pdf Yusof, Faridah and Faruck, Mohammad Omer and Chowdhury, Silvia (2017) An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response. International Food Research Journal (IFRJ), 24. pp. 457-462. ISSN 1985-4668 E-ISSN 2231-7546 http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(31)%20R1.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Yusof, Faridah Faruck, Mohammad Omer Chowdhury, Silvia An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response
description	Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius) by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield, Response Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopted to optimize the extraction process parameters. The effect of independent parameters, namely, the homogenization temperature (°C), homogenization time (min) and solid (g) to the solvent (ml) ratio of the extraction process on the fungal growth was studied. The extracted samples obtained by conducting runs accorded by the experimental design showed varying degree of antifungal activity against Aspergillus niger, the selected fungal strain, as assayed by the ‘‘Poisoned agar technique’. The investigation showed that the optimum values of the extraction parameters for the maximum antifungal peptides were 5 minutes homogenization time, 4°C homogenization temperature and 3.5:1 solid to solvent ratio. This study reports the development of an extraction process that allows careful recovery of antifungal peptides from insect larvae. In the validation of the experimental model, the error between the actual value and the predicted value was determined to be 3.57%.
format	Article
author	Yusof, Faridah Faruck, Mohammad Omer Chowdhury, Silvia
author_facet	Yusof, Faridah Faruck, Mohammad Omer Chowdhury, Silvia
author_sort	Yusof, Faridah
title	An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response
title_short	An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response
title_full	An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response
title_fullStr	An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response
title_full_unstemmed	An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response
title_sort	approach to optimize process parameter for peptides extraction from zophobas morio (fabricius) using antifungal activity as the response
publisher	Faculty of Food Science & Technology, Universiti Pertanian Malaysia
publishDate	2017
url	http://irep.iium.edu.my/59630/ http://irep.iium.edu.my/59630/ http://irep.iium.edu.my/59630/1/59630_An%20approach%20to%20optimize%20process.pdf http://irep.iium.edu.my/59630/7/59630_An%20approach%20to%20optimize%20process%20parameter_scopus.pdf
first_indexed	2023-09-18T21:24:29Z
last_indexed	2023-09-18T21:24:29Z
_version_	1777412099960471552

An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response

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