Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance
Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received c...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer Netherlands
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/1/Paper-Samsudin%20et%20al%202017%20%28Cytotech%29.pdf http://irep.iium.edu.my/59730/7/59730-Optimization%20of%20ultraviolet%20ozone%20treatment%20process%20for%20improvement%20of%20polycaprolactone_SCOPUS.pdf |
| Summary: | Growing cells on microcarriers may have
overcome the limitation of conventional cell culture
system. However, the surface functionality of certain
polymeric microcarriers for effective cell attachment
and growth remains a challenge. Polycaprolactone
(PCL), a biodegradable polymer has received considerable
attention due to its good mechanical properties and
degradation rate. The drawback is the non-polar hydrocarbon
moiety which makes it not readily suitable for
cell attachment. This report concerns the modification of
PCL microcarrier surface (introduction of functional
oxygen groups) using ultraviolet irradiation and ozone
(UV/O3) system and investigation of the effects of ozone
concentration, the amount of PCL and exposure time;
where the optimum conditions were found to be at
60,110.52 ppm, 5.5 g PCL and 60 min, respectively.
The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.92 nmol/g and the
amount of gelatin immobilized was 320 ± 0.9 lg/g on
UV/O3 treated microcarriers as compared to the
untreated (26.83 ± 3 lg/g) microcarriers. The absorption
of functional oxygen groups on the surface and the
immobilized gelatin was confirmed with the attenuated
total reflectance Fourier transformed infrared spectroscopy
(ATR-FTIR) and the enhancement of
hydrophilicity of the surface was confirmed using water
contact angle measurement which decreased (86.93–
49.34) after UV/O3 treatment and subsequently after
immobilization of gelatin. The attachment and growth
kinetics for HaCaT skin keratinocyte cells showed that
adhesion occurred much more rapidly for oxidized
surfaces and gelatin immobilized surface as compared to
untreated PCL. |
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