Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received c...

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Main Authors:	Samsudin, Nurhusna, Hashim, Yumi Zuhanis Has-Yun, Arifin, Mohd Azmir, Mel, Maizirwan, Mohd Salleh, Hamzah, Sopyan, Iis, Jimat, Dzun Noraini
Format:	Article
Language:	English English
Published:	Springer Netherlands 2017
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/1/Paper-Samsudin%20et%20al%202017%20%28Cytotech%29.pdf http://irep.iium.edu.my/59730/7/59730-Optimization%20of%20ultraviolet%20ozone%20treatment%20process%20for%20improvement%20of%20polycaprolactone_SCOPUS.pdf

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recordtype	eprints
spelling	iium-597302019-10-23T01:54:32Z http://irep.iium.edu.my/59730/ Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini Q Science (General) Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110.52 ppm, 5.5 g PCL and 60 min, respectively. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.92 nmol/g and the amount of gelatin immobilized was 320 ± 0.9 lg/g on UV/O3 treated microcarriers as compared to the untreated (26.83 ± 3 lg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93– 49.34) after UV/O3 treatment and subsequently after immobilization of gelatin. The attachment and growth kinetics for HaCaT skin keratinocyte cells showed that adhesion occurred much more rapidly for oxidized surfaces and gelatin immobilized surface as compared to untreated PCL. Springer Netherlands 2017-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59730/1/Paper-Samsudin%20et%20al%202017%20%28Cytotech%29.pdf application/pdf en http://irep.iium.edu.my/59730/7/59730-Optimization%20of%20ultraviolet%20ozone%20treatment%20process%20for%20improvement%20of%20polycaprolactone_SCOPUS.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd Salleh, Hamzah and Sopyan, Iis and Jimat, Dzun Noraini (2017) Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance. Cytotechnology, 69 (4). pp. 601-616. ISSN 0920-9069 E-ISSN 1573-0778 https://link.springer.com/article/10.1007/s10616-017-0071-x 10.1007/s10616-017-0071-x
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance
description	Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110.52 ppm, 5.5 g PCL and 60 min, respectively. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.92 nmol/g and the amount of gelatin immobilized was 320 ± 0.9 lg/g on UV/O3 treated microcarriers as compared to the untreated (26.83 ± 3 lg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93– 49.34) after UV/O3 treatment and subsequently after immobilization of gelatin. The attachment and growth kinetics for HaCaT skin keratinocyte cells showed that adhesion occurred much more rapidly for oxidized surfaces and gelatin immobilized surface as compared to untreated PCL.
format	Article
author	Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini
author_facet	Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini
author_sort	Samsudin, Nurhusna
title	Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance
title_short	Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance
title_full	Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance
title_fullStr	Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance
title_full_unstemmed	Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance
title_sort	optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (pcl) microcarrier performance
publisher	Springer Netherlands
publishDate	2017
url	http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/ http://irep.iium.edu.my/59730/1/Paper-Samsudin%20et%20al%202017%20%28Cytotech%29.pdf http://irep.iium.edu.my/59730/7/59730-Optimization%20of%20ultraviolet%20ozone%20treatment%20process%20for%20improvement%20of%20polycaprolactone_SCOPUS.pdf
first_indexed	2023-09-18T21:24:38Z
last_indexed	2023-09-18T21:24:38Z
_version_	1777412109258194944

Optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (PCL) microcarrier performance

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