Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism

Introduction: PAX9 (Paired box 9) gene is one of the genes which play significant role during craniofacial development. Single nucleotide polymorphism (SNP) in PAX9 has been associated with Class II/Division 2 malocclusion (with or without hypodontia). However, the relationship between PAX9 SNP mark...

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Main Authors:	Abu Bakar, Noraini, Mokhtar@Makhtar, Khairani Idah, Kharuddin, Azrul Fazwan
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/59807/ http://irep.iium.edu.my/59807/ http://irep.iium.edu.my/59807/13/59807-Identification%20Of%20PAX9%20Single%20Nucleotide%20Polymorphism%20in%20Class%20III.pdf

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spelling	iium-598072018-07-10T04:45:36Z http://irep.iium.edu.my/59807/ Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan RK Dentistry Introduction: PAX9 (Paired box 9) gene is one of the genes which play significant role during craniofacial development. Single nucleotide polymorphism (SNP) in PAX9 has been associated with Class II/Division 2 malocclusion (with or without hypodontia). However, the relationship between PAX9 SNP marker (rs8004560) with mandibular prognathism (MP) has not been analysed, at least in our local population. This study aimed to detect the presence of PAX9 (rs8004560) SNP in Class III malocclusion patients (with MP) in the local population. Materials and Methods: Genomic DNA were extracted from unstimulated saliva of 31 class I malocclusion (control samples) and 30 patients from Class III malocclusion (MP). Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for the marker rs8004560 and the genotyping was done by sequencing. Chi-square test was used to determine the over-representation of marker allele (p<0.05). Results: Presence of PAX9 SNP (rs8004560) was detected in local population analysed and the distribution of its genotype and allele could be observed. There were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within control (Class I) and Class III malocclusion. Conclusion(s): The most common allele of a marker flanking PAX9 (rs8004560) was over-represented in the mandibular prognathism (MP) subjects indicating the genetic association of PAX9 (rs8004560) SNP in the incidence of MP. Further studies involving larger number of samples should be developed in order to understand the exact role and mechanism of PAX9 in different classes of malocclusions. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/59807/13/59807-Identification%20Of%20PAX9%20Single%20Nucleotide%20Polymorphism%20in%20Class%20III.pdf Abu Bakar, Noraini and Mokhtar@Makhtar, Khairani Idah and Kharuddin, Azrul Fazwan (2017) Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism. In: 1st IIUM International Dental Conference 2017 in Commemoration of the Kulliyyah of Dentistry 10th Anniversary (1st IIDC), 6th-8th October 2017, Kuantan, Pahang. http://iiumedic.net/imjm/v1/download/Volume%2016%20No%201%20Supp%202/ID56_IMJM-Vol16-Supp2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism
description	Introduction: PAX9 (Paired box 9) gene is one of the genes which play significant role during craniofacial development. Single nucleotide polymorphism (SNP) in PAX9 has been associated with Class II/Division 2 malocclusion (with or without hypodontia). However, the relationship between PAX9 SNP marker (rs8004560) with mandibular prognathism (MP) has not been analysed, at least in our local population. This study aimed to detect the presence of PAX9 (rs8004560) SNP in Class III malocclusion patients (with MP) in the local population. Materials and Methods: Genomic DNA were extracted from unstimulated saliva of 31 class I malocclusion (control samples) and 30 patients from Class III malocclusion (MP). Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for the marker rs8004560 and the genotyping was done by sequencing. Chi-square test was used to determine the over-representation of marker allele (p<0.05). Results: Presence of PAX9 SNP (rs8004560) was detected in local population analysed and the distribution of its genotype and allele could be observed. There were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within control (Class I) and Class III malocclusion. Conclusion(s): The most common allele of a marker flanking PAX9 (rs8004560) was over-represented in the mandibular prognathism (MP) subjects indicating the genetic association of PAX9 (rs8004560) SNP in the incidence of MP. Further studies involving larger number of samples should be developed in order to understand the exact role and mechanism of PAX9 in different classes of malocclusions.
format	Conference or Workshop Item
author	Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan
author_facet	Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan
author_sort	Abu Bakar, Noraini
title	Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism
title_short	Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism
title_full	Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism
title_fullStr	Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism
title_full_unstemmed	Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism
title_sort	identification of pax9 single nucleotide polymorphism in class iii malocclusion patients with mandibular prognatism
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/59807/ http://irep.iium.edu.my/59807/ http://irep.iium.edu.my/59807/13/59807-Identification%20Of%20PAX9%20Single%20Nucleotide%20Polymorphism%20in%20Class%20III.pdf
first_indexed	2023-09-18T21:24:45Z
last_indexed	2023-09-18T21:24:45Z
_version_	1777412117198012416

Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism

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