Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome

Treatment of osteomyelitis is still a major problem in orthopaedic field. New Zealand White rabbit (NZWR) is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of this study w...

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Main Authors:	Zulkifly, Ahmad Hafiz, Mohd Jan, Nurul Hafiza, Aziz, Aziah, Ibrahim, Mohd Zulfadzli
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	RD701 Orthopedics
Online Access:	http://irep.iium.edu.my/60162/ http://irep.iium.edu.my/60162/ http://irep.iium.edu.my/60162/21/60162-Osteomyelitis%20treatment%20in%20New%20Zealand%20white%20rabbit%20using%20impregnated%20biomaterials.pdf

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recordtype	eprints
spelling	iium-601622018-01-17T03:13:16Z http://irep.iium.edu.my/60162/ Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Aziz, Aziah Ibrahim, Mohd Zulfadzli RD701 Orthopedics Treatment of osteomyelitis is still a major problem in orthopaedic field. New Zealand White rabbit (NZWR) is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of this study was to analyze the treatment given via impregnated biomaterials with gentamicin beads in rabbit models. Osteomyelitis was created by inoculation of Staphylococcus aureus ATCC 25923 into the drilled rabbit’s distal femur. Thirty-four (32) NZWR was used during the research. The rabbits were divided into 2 groups; (hydroxyapatite (HA) and calcium sulphate (Ca2SO4)) with four subgroups assessments in (3, 6, 12 and 26) weeks. The response of the treatments against infection was evaluated through gross presentations, radiograph, micro CT, microbiology and histological. The rabbits were sacrificed accordingly to evaluate the healing process of bone. Results showed osteomyelitis changes were noted in all rabbit after inoculation of bacteria at week 3 intervals. Intervention was done after 3 weeks of inoculation. Chronic osteomyelitis was confirmed via radiology and microbial assessments. SPSS was used to evaluate the results. The microbiology analysis revealed there was no bacterium isolated at 26th week interval. In histological interpretation, healing was noted at infected area with appearance of new bone formation at 6 to 26 week of intervals. The micro CT results showed that trebaculae numbers increased significantly (p<0.05) with the treatment. The results show bone healing at 26th week of interval. There was no significant difference between both treatments in the area of radiology, histology and micro CT assessment (p<0.05). The impregnated antibiotics biomaterial showed its function in the treatment of osteomyelitis. In conclusion, these result showed that impregnated biomaterials with gentamicin have roles in treatment of osteomyelitis as added to medical and surgical treatment 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/60162/21/60162-Osteomyelitis%20treatment%20in%20New%20Zealand%20white%20rabbit%20using%20impregnated%20biomaterials.pdf Zulkifly, Ahmad Hafiz and Mohd Jan, Nurul Hafiza and Aziz, Aziah and Ibrahim, Mohd Zulfadzli (2017) Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome. In: 1st Postgraduate symposium on Tissue Engineering and Regenerative Medicine 2017 (TERMSYMPO 2017), 7th November 2017, Cheras, Kuala Lumpur. (Unpublished) https://termsympo2017.files.wordpress.com/2017/08/abstract-book.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RD701 Orthopedics
spellingShingle	RD701 Orthopedics Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Aziz, Aziah Ibrahim, Mohd Zulfadzli Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome
description	Treatment of osteomyelitis is still a major problem in orthopaedic field. New Zealand White rabbit (NZWR) is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of this study was to analyze the treatment given via impregnated biomaterials with gentamicin beads in rabbit models. Osteomyelitis was created by inoculation of Staphylococcus aureus ATCC 25923 into the drilled rabbit’s distal femur. Thirty-four (32) NZWR was used during the research. The rabbits were divided into 2 groups; (hydroxyapatite (HA) and calcium sulphate (Ca2SO4)) with four subgroups assessments in (3, 6, 12 and 26) weeks. The response of the treatments against infection was evaluated through gross presentations, radiograph, micro CT, microbiology and histological. The rabbits were sacrificed accordingly to evaluate the healing process of bone. Results showed osteomyelitis changes were noted in all rabbit after inoculation of bacteria at week 3 intervals. Intervention was done after 3 weeks of inoculation. Chronic osteomyelitis was confirmed via radiology and microbial assessments. SPSS was used to evaluate the results. The microbiology analysis revealed there was no bacterium isolated at 26th week interval. In histological interpretation, healing was noted at infected area with appearance of new bone formation at 6 to 26 week of intervals. The micro CT results showed that trebaculae numbers increased significantly (p<0.05) with the treatment. The results show bone healing at 26th week of interval. There was no significant difference between both treatments in the area of radiology, histology and micro CT assessment (p<0.05). The impregnated antibiotics biomaterial showed its function in the treatment of osteomyelitis. In conclusion, these result showed that impregnated biomaterials with gentamicin have roles in treatment of osteomyelitis as added to medical and surgical treatment
format	Conference or Workshop Item
author	Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Aziz, Aziah Ibrahim, Mohd Zulfadzli
author_facet	Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Aziz, Aziah Ibrahim, Mohd Zulfadzli
author_sort	Zulkifly, Ahmad Hafiz
title	Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome
title_short	Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome
title_full	Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome
title_fullStr	Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome
title_full_unstemmed	Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome
title_sort	osteomyelitis treatment in new zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome
publishDate	2017
url	http://irep.iium.edu.my/60162/ http://irep.iium.edu.my/60162/ http://irep.iium.edu.my/60162/21/60162-Osteomyelitis%20treatment%20in%20New%20Zealand%20white%20rabbit%20using%20impregnated%20biomaterials.pdf
first_indexed	2023-09-18T21:25:16Z
last_indexed	2023-09-18T21:25:16Z
_version_	1777412149667168256

Osteomyelitis treatment in New Zealand white rabbit using impregnated biomaterials with gentamicin– in vivo studies outcome

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