Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria

Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria

Antibacterial activity of different types of P. odorata leaf extracts was evaluated in combination with standard antibiotics. Persicaria. odorata leaves were extracted with n-hexane (n-hex), dichloromethane (DCM) and methanol (MeOH). Each extract was applied on vancomycin (30μg), erythromycin (15μg)...

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Main Authors: Ridzuan, P. M., Hamzah, Hairul Aini, Shah, Anis, Mohd Hassan, Norazian, Baharudin, Roesnita
Format: Article
Language:English
English
Published: International Islamic University Malaysia Press 2017
Subjects:
QR Microbiology
Online Access:http://irep.iium.edu.my/60747/
http://irep.iium.edu.my/60747/
http://irep.iium.edu.my/60747/1/IMJM-Vol16-No2-2732.pdf
http://irep.iium.edu.my/60747/7/Synergistic%20effects%20of%20Persicaria%20odorata%20%28Daun%20Kesom%29%20leaf%20extracts%20with%20standard%20antibiotics%20on%20pathogenic%20bacteria.pdf
id iium-60747
recordtype eprints
spelling iium-607472018-01-23T07:46:47Z http://irep.iium.edu.my/60747/ Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria Ridzuan, P. M. Hamzah, Hairul Aini Shah, Anis Mohd Hassan, Norazian Baharudin, Roesnita QR Microbiology Antibacterial activity of different types of P. odorata leaf extracts was evaluated in combination with standard antibiotics. Persicaria. odorata leaves were extracted with n-hexane (n-hex), dichloromethane (DCM) and methanol (MeOH). Each extract was applied on vancomycin (30μg), erythromycin (15μg) and gentamicin (10μg) discs, respectively. Disk diffusion method was used to evaluate the synergistic activity of each combination on Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Minimum inhibitory concentration (MIC) and gas chromatography mass spectrometry (GCMS) analysis was performed on the active extract. Synergistic effects seen were mainly from the n-hex+antibiotics combinations, mainly on the Gram-positive bacteria (7 additive, 5 antagonistic), with MIC range from 50 μg/ml to 100 μg/ml, as well as Gram-negative bacteria (2 additive, 2 indifferent, 5 antagonistic). In particular, synergism showed by the combination of n-hex+van were all additive against the susceptible bacteria. DCM extract combination showed synergistic effects on three Gram-positive species (S. aureus, S. epidermidis, S. pyogenes). Meanwhile, MeOH+antibiotics combination showed significant additive synergistic effects (p<0.05) on S. aureus and S. epidermidis. The major compounds of leaves extract were decanal and β-citral. n-Hex extract superiorly inhibited Gram-positive bacteria growth as compared to DCM and MeOH extracts. The additive synergistic property of the n-hex P. odorata extract could be further studied for possible use as an antibacterial agent. International Islamic University Malaysia Press 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60747/1/IMJM-Vol16-No2-2732.pdf application/pdf en http://irep.iium.edu.my/60747/7/Synergistic%20effects%20of%20Persicaria%20odorata%20%28Daun%20Kesom%29%20leaf%20extracts%20with%20standard%20antibiotics%20on%20pathogenic%20bacteria.pdf Ridzuan, P. M. and Hamzah, Hairul Aini and Shah, Anis and Mohd Hassan, Norazian and Baharudin, Roesnita (2017) Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria. The International Medical Journal Malaysia, 16 (2). pp. 27-32. ISSN 1823-4631 http://iiumedic.net/imjm/v1/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic QR Microbiology
spellingShingle QR Microbiology
Ridzuan, P. M.
Hamzah, Hairul Aini
Shah, Anis
Mohd Hassan, Norazian
Baharudin, Roesnita
Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria
description Antibacterial activity of different types of P. odorata leaf extracts was evaluated in combination with standard antibiotics. Persicaria. odorata leaves were extracted with n-hexane (n-hex), dichloromethane (DCM) and methanol (MeOH). Each extract was applied on vancomycin (30μg), erythromycin (15μg) and gentamicin (10μg) discs, respectively. Disk diffusion method was used to evaluate the synergistic activity of each combination on Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Minimum inhibitory concentration (MIC) and gas chromatography mass spectrometry (GCMS) analysis was performed on the active extract. Synergistic effects seen were mainly from the n-hex+antibiotics combinations, mainly on the Gram-positive bacteria (7 additive, 5 antagonistic), with MIC range from 50 μg/ml to 100 μg/ml, as well as Gram-negative bacteria (2 additive, 2 indifferent, 5 antagonistic). In particular, synergism showed by the combination of n-hex+van were all additive against the susceptible bacteria. DCM extract combination showed synergistic effects on three Gram-positive species (S. aureus, S. epidermidis, S. pyogenes). Meanwhile, MeOH+antibiotics combination showed significant additive synergistic effects (p<0.05) on S. aureus and S. epidermidis. The major compounds of leaves extract were decanal and β-citral. n-Hex extract superiorly inhibited Gram-positive bacteria growth as compared to DCM and MeOH extracts. The additive synergistic property of the n-hex P. odorata extract could be further studied for possible use as an antibacterial agent.
format Article
author Ridzuan, P. M.
Hamzah, Hairul Aini
Shah, Anis
Mohd Hassan, Norazian
Baharudin, Roesnita
author_facet Ridzuan, P. M.
Hamzah, Hairul Aini
Shah, Anis
Mohd Hassan, Norazian
Baharudin, Roesnita
author_sort Ridzuan, P. M.
title Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria
title_short Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria
title_full Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria
title_fullStr Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria
title_full_unstemmed Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria
title_sort synergistic effects of persicaria odorata (daun kesom) leaf extracts with standard antibiotics on pathogenic bacteria
publisher International Islamic University Malaysia Press
publishDate 2017
url http://irep.iium.edu.my/60747/
http://irep.iium.edu.my/60747/
http://irep.iium.edu.my/60747/1/IMJM-Vol16-No2-2732.pdf
http://irep.iium.edu.my/60747/7/Synergistic%20effects%20of%20Persicaria%20odorata%20%28Daun%20Kesom%29%20leaf%20extracts%20with%20standard%20antibiotics%20on%20pathogenic%20bacteria.pdf
first_indexed 2023-09-18T21:26:07Z
last_indexed 2023-09-18T21:26:07Z
_version_ 1777412202613964800

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