Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs

ABSTRACT Tissue engineering has emerged as a new promising field that allow in vitro construction of whole transplantable tissue. Recently, bone marrow stem cells (BMSCs) fulfil the requirements as appropriate cell source that is renewable for cartilage tissue engineering since they were able to fo...

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Main Authors:	Alfaqeh, Hamoud Hussein Mohammed, Idrus, Ruszymah, Chua, Kein Hui, Saim, Aminuddin
Format:	Article
Language:	English
Published:	UKM 2017
Subjects:	QM Human anatomy QP Physiology
Online Access:	http://irep.iium.edu.my/60800/ http://irep.iium.edu.my/60800/ http://irep.iium.edu.my/60800/1/13_hamoud_et_al_pdf_44993%20Decdember%202017.pdf

id	iium-60800
recordtype	eprints
spelling	iium-608002018-03-20T01:36:28Z http://irep.iium.edu.my/60800/ Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs Alfaqeh, Hamoud Hussein Mohammed Idrus, Ruszymah Chua, Kein Hui Saim, Aminuddin QM Human anatomy QP Physiology ABSTRACT Tissue engineering has emerged as a new promising field that allow in vitro construction of whole transplantable tissue. Recently, bone marrow stem cells (BMSCs) fulfil the requirements as appropriate cell source that is renewable for cartilage tissue engineering since they were able to form hyaline-like cartilage in vitro and in vivo when cultured in media supplemented with specific growth factors. This study aimed to examine the potential of BMSCs chondrogenesis in vitro and in vivo. BMSCs were isolated from sheep and divided into a test group and control group. Control group was cultured in medium supplemented with 10% fetal bovine serum (FBS). Test group was cultured in medium supplemented with 1% FBS, 1% ITS, 5ng/mL TGF-ß3, 50ng/mL IGF-1, 40 ng/mL L-proline, 100 nM dexamethasone and 50 μg/mL ascorbic acid-2 phosphate. The experiment was carried out on athymic nude mice. Cell aggregates were formed in test group and indicated the early chondrogenesis in cell culture. Later, cells-fibrin constructs were made and implanted subcutaneously into nude mice for 5 wks, then explanted for histological examination and glycosaminoglycans (GAG) quantification. Test group constructs showed higher cartilage matrix synthesis as confirmed by Safranin O staining and GAG production. These results demonstrated the effectiveness of low serum media supplemented with TGF-ß3 in promoting chondrogenesis in BMSCs. Keywords: bone marrow, cartilage, chondrogenesis, growth factors, low serum media, stem cells UKM 2017-12-22 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60800/1/13_hamoud_et_al_pdf_44993%20Decdember%202017.pdf Alfaqeh, Hamoud Hussein Mohammed and Idrus, Ruszymah and Chua, Kein Hui and Saim, Aminuddin (2017) Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs. Medicine and Health, 12 (2). pp. 293-303. E-ISSN 2289-5728 http://www.medicineandhealthukm.com/content/aims-and-scope
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QM Human anatomy QP Physiology
spellingShingle	QM Human anatomy QP Physiology Alfaqeh, Hamoud Hussein Mohammed Idrus, Ruszymah Chua, Kein Hui Saim, Aminuddin Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
description	ABSTRACT Tissue engineering has emerged as a new promising field that allow in vitro construction of whole transplantable tissue. Recently, bone marrow stem cells (BMSCs) fulfil the requirements as appropriate cell source that is renewable for cartilage tissue engineering since they were able to form hyaline-like cartilage in vitro and in vivo when cultured in media supplemented with specific growth factors. This study aimed to examine the potential of BMSCs chondrogenesis in vitro and in vivo. BMSCs were isolated from sheep and divided into a test group and control group. Control group was cultured in medium supplemented with 10% fetal bovine serum (FBS). Test group was cultured in medium supplemented with 1% FBS, 1% ITS, 5ng/mL TGF-ß3, 50ng/mL IGF-1, 40 ng/mL L-proline, 100 nM dexamethasone and 50 μg/mL ascorbic acid-2 phosphate. The experiment was carried out on athymic nude mice. Cell aggregates were formed in test group and indicated the early chondrogenesis in cell culture. Later, cells-fibrin constructs were made and implanted subcutaneously into nude mice for 5 wks, then explanted for histological examination and glycosaminoglycans (GAG) quantification. Test group constructs showed higher cartilage matrix synthesis as confirmed by Safranin O staining and GAG production. These results demonstrated the effectiveness of low serum media supplemented with TGF-ß3 in promoting chondrogenesis in BMSCs. Keywords: bone marrow, cartilage, chondrogenesis, growth factors, low serum media, stem cells
format	Article
author	Alfaqeh, Hamoud Hussein Mohammed Idrus, Ruszymah Chua, Kein Hui Saim, Aminuddin
author_facet	Alfaqeh, Hamoud Hussein Mohammed Idrus, Ruszymah Chua, Kein Hui Saim, Aminuddin
author_sort	Alfaqeh, Hamoud Hussein Mohammed
title	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_short	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_full	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_fullStr	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_full_unstemmed	Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs
title_sort	supplementation of tgf-beta3 in low serum media promotes chondrogenesis of bmscs
publisher	UKM
publishDate	2017
url	http://irep.iium.edu.my/60800/ http://irep.iium.edu.my/60800/ http://irep.iium.edu.my/60800/1/13_hamoud_et_al_pdf_44993%20Decdember%202017.pdf
first_indexed	2023-09-18T21:26:11Z
last_indexed	2023-09-18T21:26:11Z
_version_	1777412207202533376

Supplementation of TGF-Beta3 in low serum media promotes chondrogenesis of BMSCs

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