Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
In recent years, the search for natural, safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/60854/ http://irep.iium.edu.my/60854/ http://irep.iium.edu.my/60854/1/60854_Effects%20of%20Sargassum%20oligocystum.pdf http://irep.iium.edu.my/60854/7/60854_Effects%20of%20Sargassum%20oligocystum%20and%20Padina_new_scopus.pdf |
| Summary: | In recent years, the search for natural, safe and effective therapies for the management of
obesity has become important. The present study investigated the potential of brown seaweeds
Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for
the management of obesity. Both seaweeds were macerated using acetone at room temperature
for ten hours. The S. oligocystum extracts (SE) and P. australis extracts (PE) were then applied
to 3T3-L1 cells during the differentiation stage and during the mature stage of the adipocyte life
cycle to assess the effects of extracts on adipogenesis and adipolysis. Application of SE at 12.5
and 50 µg/ml to the cells decreased adipogenesis by 71.7%, and 84.8%, respectively, while
cells treated with 12.5 and 50 µg/ml PE showed 85.7%, and 89.0% adipogenesis respectively,
compared to control. Application of SE and PE to mature lipid cells stimulated adipolysis
and the release of glycerol into the culture media. Application of SE at 12.5 and 50 µg µg/
ml in the cell media induced glycerol release by up to 88.6 and 93.0%, respectively, while PE
increased glycerol release up to 92.9 and 95.6% respectively, compared to isoproterenol. This
study demonstrates the potential of whole brown seaweed extracts from S. oligocystum and P.
australis collected from Malaysian waters as natural anti- obesity agents. Incorporation of the
brown seaweed into the diet as a functional food will be useful for prevention and treatment
of obesity. |
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