Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells

In recent years, the search for natural, safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of o...

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Main Authors:	Jaswir, Irwandi, Ahmad, Hasna, Darnis, Deny Susanti, Bakhtiar, M. Taher, Octavianti, Fitri
Format:	Article
Language:	English English
Published:	Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017
Subjects:	QD Chemistry TP Chemical technology
Online Access:	http://irep.iium.edu.my/60854/ http://irep.iium.edu.my/60854/ http://irep.iium.edu.my/60854/1/60854_Effects%20of%20Sargassum%20oligocystum.pdf http://irep.iium.edu.my/60854/7/60854_Effects%20of%20Sargassum%20oligocystum%20and%20Padina_new_scopus.pdf

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recordtype	eprints
spelling	iium-608542018-04-19T13:39:11Z http://irep.iium.edu.my/60854/ Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells Jaswir, Irwandi Ahmad, Hasna Darnis, Deny Susanti Bakhtiar, M. Taher Octavianti, Fitri QD Chemistry TP Chemical technology In recent years, the search for natural, safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of obesity. Both seaweeds were macerated using acetone at room temperature for ten hours. The S. oligocystum extracts (SE) and P. australis extracts (PE) were then applied to 3T3-L1 cells during the differentiation stage and during the mature stage of the adipocyte life cycle to assess the effects of extracts on adipogenesis and adipolysis. Application of SE at 12.5 and 50 µg/ml to the cells decreased adipogenesis by 71.7%, and 84.8%, respectively, while cells treated with 12.5 and 50 µg/ml PE showed 85.7%, and 89.0% adipogenesis respectively, compared to control. Application of SE and PE to mature lipid cells stimulated adipolysis and the release of glycerol into the culture media. Application of SE at 12.5 and 50 µg µg/ ml in the cell media induced glycerol release by up to 88.6 and 93.0%, respectively, while PE increased glycerol release up to 92.9 and 95.6% respectively, compared to isoproterenol. This study demonstrates the potential of whole brown seaweed extracts from S. oligocystum and P. australis collected from Malaysian waters as natural anti- obesity agents. Incorporation of the brown seaweed into the diet as a functional food will be useful for prevention and treatment of obesity. Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60854/1/60854_Effects%20of%20Sargassum%20oligocystum.pdf application/pdf en http://irep.iium.edu.my/60854/7/60854_Effects%20of%20Sargassum%20oligocystum%20and%20Padina_new_scopus.pdf Jaswir, Irwandi and Ahmad, Hasna and Darnis, Deny Susanti and Bakhtiar, M. Taher and Octavianti, Fitri (2017) Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells. International Food Research Journal, 24 (Suppl.). pp. 395-401. ISSN 1985-4668 E-ISSN 2231-7546 http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(21)%20R1.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry TP Chemical technology
spellingShingle	QD Chemistry TP Chemical technology Jaswir, Irwandi Ahmad, Hasna Darnis, Deny Susanti Bakhtiar, M. Taher Octavianti, Fitri Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
description	In recent years, the search for natural, safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of obesity. Both seaweeds were macerated using acetone at room temperature for ten hours. The S. oligocystum extracts (SE) and P. australis extracts (PE) were then applied to 3T3-L1 cells during the differentiation stage and during the mature stage of the adipocyte life cycle to assess the effects of extracts on adipogenesis and adipolysis. Application of SE at 12.5 and 50 µg/ml to the cells decreased adipogenesis by 71.7%, and 84.8%, respectively, while cells treated with 12.5 and 50 µg/ml PE showed 85.7%, and 89.0% adipogenesis respectively, compared to control. Application of SE and PE to mature lipid cells stimulated adipolysis and the release of glycerol into the culture media. Application of SE at 12.5 and 50 µg µg/ ml in the cell media induced glycerol release by up to 88.6 and 93.0%, respectively, while PE increased glycerol release up to 92.9 and 95.6% respectively, compared to isoproterenol. This study demonstrates the potential of whole brown seaweed extracts from S. oligocystum and P. australis collected from Malaysian waters as natural anti- obesity agents. Incorporation of the brown seaweed into the diet as a functional food will be useful for prevention and treatment of obesity.
format	Article
author	Jaswir, Irwandi Ahmad, Hasna Darnis, Deny Susanti Bakhtiar, M. Taher Octavianti, Fitri
author_facet	Jaswir, Irwandi Ahmad, Hasna Darnis, Deny Susanti Bakhtiar, M. Taher Octavianti, Fitri
author_sort	Jaswir, Irwandi
title	Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_short	Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_full	Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_fullStr	Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_full_unstemmed	Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells
title_sort	effects of sargassum oligocystum and padina australis extract on adipogenesis and adipolysis in 3t3-l1 cells
publisher	Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
publishDate	2017
url	http://irep.iium.edu.my/60854/ http://irep.iium.edu.my/60854/ http://irep.iium.edu.my/60854/1/60854_Effects%20of%20Sargassum%20oligocystum.pdf http://irep.iium.edu.my/60854/7/60854_Effects%20of%20Sargassum%20oligocystum%20and%20Padina_new_scopus.pdf
first_indexed	2023-09-18T21:26:16Z
last_indexed	2023-09-18T21:26:16Z
_version_	1777412212600602624

Effects of Sargassum oligocystum and Padina australis extract on Adipogenesis and Adipolysis in 3T3-L1 Cells

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