Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP)

The use of HPP as an extraction method was studied by evaluating yield of astaxanthin from shrimp carapace as a model. Previous studies have demonstrated the antioxidant and antimicrobial properties of asatxanthin. The aim of this research was to compare these properties of astxanthin as a surrogate...

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Main Authors:	Irna, Cicy, Jaswir, Irwandi, Othman, Rashidi, Jimat, Dzun Noraini
Format:	Article
Language:	English English
Published:	Universiti Putra Malaysia 2017
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/60857/ http://irep.iium.edu.my/60857/ http://irep.iium.edu.my/60857/1/60857_Antioxidant%20and%20Antimicrobial%20Activities.pdf http://irep.iium.edu.my/60857/7/60857_Antioxidant%20and%20antimicrobial%20activities_scopus.pdf

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spelling	iium-608572018-04-03T01:04:22Z http://irep.iium.edu.my/60857/ Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP) Irna, Cicy Jaswir, Irwandi Othman, Rashidi Jimat, Dzun Noraini TP248.13 Biotechnology The use of HPP as an extraction method was studied by evaluating yield of astaxanthin from shrimp carapace as a model. Previous studies have demonstrated the antioxidant and antimicrobial properties of asatxanthin. The aim of this research was to compare these properties of astxanthin as a surrogate for its yield from High Pressure Processing (HPP) extraction with the effect of hydrostatic pressure, holding time and amount of solvents versus a more conventional chemical extraction method. A solvent mixture of acetone and methanol (7:3, v/v) was used in both methods. The pressure treated was at 238 MPa with 16.29 mins of holding time and 6.59 ml of solvents for HPP method. Antioxidant activity was evaluated using scavenging activity of DPPH radical, the reducing activity of ferrum redox reaction and oxygen radical absorption capacity. Antimicrobial activity was evaluated using a zone of inhibition test against four strain of bacteria : E. coli, E. aerogenes, S. aureus and B. subtilis. The sample of asatxanthin demonstrated a significant increase in DPPH radical scavenging activity (25.47% to 87.90%), reducing activity of ferrum redox reaction (2.86 µmol TE/g to 8.13 µmol TE/g) and oxygen radical absorption capacity (2,000 µmol TE/100 g to 4,000 µmol TE/100 g) compared to the chemical extraction sample. The antimicrobial activity of the astaxathin from the HPP sample produced greater zone of inhibition against all four strains of bacteria when compared to the chemically extracted sample. A higher quality of astaxanthin was achieved with the HPP extraction method compared to chemical extraction. Universiti Putra Malaysia 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60857/1/60857_Antioxidant%20and%20Antimicrobial%20Activities.pdf application/pdf en http://irep.iium.edu.my/60857/7/60857_Antioxidant%20and%20antimicrobial%20activities_scopus.pdf Irna, Cicy and Jaswir, Irwandi and Othman, Rashidi and Jimat, Dzun Noraini (2017) Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP). International Food Research Journal (IFRJ), 24. pp. 508-513. ISSN 1985-4668 E-ISSN 2231-7546 http://ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(40)%20R1.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Irna, Cicy Jaswir, Irwandi Othman, Rashidi Jimat, Dzun Noraini Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP)
description	The use of HPP as an extraction method was studied by evaluating yield of astaxanthin from shrimp carapace as a model. Previous studies have demonstrated the antioxidant and antimicrobial properties of asatxanthin. The aim of this research was to compare these properties of astxanthin as a surrogate for its yield from High Pressure Processing (HPP) extraction with the effect of hydrostatic pressure, holding time and amount of solvents versus a more conventional chemical extraction method. A solvent mixture of acetone and methanol (7:3, v/v) was used in both methods. The pressure treated was at 238 MPa with 16.29 mins of holding time and 6.59 ml of solvents for HPP method. Antioxidant activity was evaluated using scavenging activity of DPPH radical, the reducing activity of ferrum redox reaction and oxygen radical absorption capacity. Antimicrobial activity was evaluated using a zone of inhibition test against four strain of bacteria : E. coli, E. aerogenes, S. aureus and B. subtilis. The sample of asatxanthin demonstrated a significant increase in DPPH radical scavenging activity (25.47% to 87.90%), reducing activity of ferrum redox reaction (2.86 µmol TE/g to 8.13 µmol TE/g) and oxygen radical absorption capacity (2,000 µmol TE/100 g to 4,000 µmol TE/100 g) compared to the chemical extraction sample. The antimicrobial activity of the astaxathin from the HPP sample produced greater zone of inhibition against all four strains of bacteria when compared to the chemically extracted sample. A higher quality of astaxanthin was achieved with the HPP extraction method compared to chemical extraction.
format	Article
author	Irna, Cicy Jaswir, Irwandi Othman, Rashidi Jimat, Dzun Noraini
author_facet	Irna, Cicy Jaswir, Irwandi Othman, Rashidi Jimat, Dzun Noraini
author_sort	Irna, Cicy
title	Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP)
title_short	Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP)
title_full	Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP)
title_fullStr	Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP)
title_full_unstemmed	Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP)
title_sort	antioxidant and antimicrobial activities of astaxanthin from penaeus monodon in comparison between chemical extraction and high pressure processing (hpp)
publisher	Universiti Putra Malaysia
publishDate	2017
url	http://irep.iium.edu.my/60857/ http://irep.iium.edu.my/60857/ http://irep.iium.edu.my/60857/1/60857_Antioxidant%20and%20Antimicrobial%20Activities.pdf http://irep.iium.edu.my/60857/7/60857_Antioxidant%20and%20antimicrobial%20activities_scopus.pdf
first_indexed	2023-09-18T21:26:17Z
last_indexed	2023-09-18T21:26:17Z
_version_	1777412212878475264

Antioxidant and antimicrobial activities of astaxanthin from Penaeus monodon in comparison between chemical extraction and High Pressure Processing (HPP)

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