Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers.

Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers.

Arborine, a quinazoline and graveoline, a 4-quinolone are the antimicrobial active Rutaceous alkaloids isolated from Glycosmis pentaphylla and Ruta angustifolia, respectively. Both alkaloids possess chromophore with a few structural similarities with the pharmacophore of 4-quinolone antimicrobial a...

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Main Authors: Md Taib, Nurhaya, Mohd Kamal, Laina Zarisa, Mohd Hassan, Norazian, Khin Soe, May
Format: Conference or Workshop Item
Language:English
English
English
Published: University of Malaya 2017
Subjects:
QD Chemistry
QR Microbiology
RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:http://irep.iium.edu.my/60902/
http://irep.iium.edu.my/60902/1/IMFSC2017-1.pdf
http://irep.iium.edu.my/60902/2/CERT.-AZIAN.pdf
http://irep.iium.edu.my/60902/3/POSTER.pdf
id iium-60902
recordtype eprints
spelling iium-609022018-01-01T08:41:35Z http://irep.iium.edu.my/60902/ Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers. Md Taib, Nurhaya Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Khin Soe, May QD Chemistry QR Microbiology RS403 Materia Medica-Pharmaceutical Chemistry Arborine, a quinazoline and graveoline, a 4-quinolone are the antimicrobial active Rutaceous alkaloids isolated from Glycosmis pentaphylla and Ruta angustifolia, respectively. Both alkaloids possess chromophore with a few structural similarities with the pharmacophore of 4-quinolone antimicrobial agent. Their bacterial DNA-binding activity was assessed by photo activated DNA binding assay and agarose gel electrophoresis. Ten different restriction enzymes which recognise and cleave DNA in a sequence-specific manner were competed by the alkaloids. The DNA binding activity was detected as inhibition of the enzymatic restriction resulting in the detection of uncleaved DNA fragments of the original size. Arborine showed inhibitory activity against the restriction enzymes EcoR I, Pae I and Dra I with the highest intensity of inhibition for Dra I which have 5’-TpA sequence. Graveoline was active against EcoR I, Dra I and Pst I. Ciprofloxacin, the second generation of quinolone antimicrobial agent only the inhibitor of Kpn I and Pst I. The photo activated bacterial DNA-binding activity was in the sequence of arborine>graveoline>ciprofloxacin. This finding revealed the potential of arborine and graveoline as lead compounds for future development of quinolone antimicrobial agents in resolving the antibiotic resistance cases which are globally common nowadays in clinical setting. University of Malaya 2017-12 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/60902/1/IMFSC2017-1.pdf application/pdf en http://irep.iium.edu.my/60902/2/CERT.-AZIAN.pdf application/pdf en http://irep.iium.edu.my/60902/3/POSTER.pdf Md Taib, Nurhaya and Mohd Kamal, Laina Zarisa and Mohd Hassan, Norazian and Khin Soe, May (2017) Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers. In: International Malaysian-French Scientific Conference (IMFSC 2017), 18-19 Dec 2017, Kuala Lumpur, Malaysia. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
topic QD Chemistry
QR Microbiology
RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle QD Chemistry
QR Microbiology
RS403 Materia Medica-Pharmaceutical Chemistry
Md Taib, Nurhaya
Mohd Kamal, Laina Zarisa
Mohd Hassan, Norazian
Khin Soe, May
Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers.
description Arborine, a quinazoline and graveoline, a 4-quinolone are the antimicrobial active Rutaceous alkaloids isolated from Glycosmis pentaphylla and Ruta angustifolia, respectively. Both alkaloids possess chromophore with a few structural similarities with the pharmacophore of 4-quinolone antimicrobial agent. Their bacterial DNA-binding activity was assessed by photo activated DNA binding assay and agarose gel electrophoresis. Ten different restriction enzymes which recognise and cleave DNA in a sequence-specific manner were competed by the alkaloids. The DNA binding activity was detected as inhibition of the enzymatic restriction resulting in the detection of uncleaved DNA fragments of the original size. Arborine showed inhibitory activity against the restriction enzymes EcoR I, Pae I and Dra I with the highest intensity of inhibition for Dra I which have 5’-TpA sequence. Graveoline was active against EcoR I, Dra I and Pst I. Ciprofloxacin, the second generation of quinolone antimicrobial agent only the inhibitor of Kpn I and Pst I. The photo activated bacterial DNA-binding activity was in the sequence of arborine>graveoline>ciprofloxacin. This finding revealed the potential of arborine and graveoline as lead compounds for future development of quinolone antimicrobial agents in resolving the antibiotic resistance cases which are globally common nowadays in clinical setting.
format Conference or Workshop Item
author Md Taib, Nurhaya
Mohd Kamal, Laina Zarisa
Mohd Hassan, Norazian
Khin Soe, May
author_facet Md Taib, Nurhaya
Mohd Kamal, Laina Zarisa
Mohd Hassan, Norazian
Khin Soe, May
author_sort Md Taib, Nurhaya
title Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers.
title_short Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers.
title_full Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers.
title_fullStr Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers.
title_full_unstemmed Photo activated bacterial DNA-binding active Rutaceous alkaloids from Glycosmis pentaphylla (Retz.) DC. and Ruta angustifolia (L.) Pers.
title_sort photo activated bacterial dna-binding active rutaceous alkaloids from glycosmis pentaphylla (retz.) dc. and ruta angustifolia (l.) pers.
publisher University of Malaya
publishDate 2017
url http://irep.iium.edu.my/60902/
http://irep.iium.edu.my/60902/1/IMFSC2017-1.pdf
http://irep.iium.edu.my/60902/2/CERT.-AZIAN.pdf
http://irep.iium.edu.my/60902/3/POSTER.pdf
first_indexed 2023-09-18T21:26:21Z
last_indexed 2023-09-18T21:26:21Z
_version_ 1777412217989234688

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