Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7)

Purpose: Currently there were many treatments available to treat cancer patients, for instance chemotherapy, radiation therapy, immune therapy, and many more. Unfortunately, advancement in cancer therapy has resulted in increasing numbers of survivors which left to deal with side effects of their...

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Main Authors:	Firus Khan, Al'aina Yuhainis, Jalal, Tara Kamal, Abdull Rasad, Mohammad Syaiful Bahari, Hashim, Ridzwan, Abdul Wahab, Ridhwan
Format:	Article
Language:	English
Published:	USM 2016
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/60912/ http://irep.iium.edu.my/60912/ http://irep.iium.edu.my/60912/1/amdi%20.pdf

id	iium-60912
recordtype	eprints
spelling	iium-609122018-07-10T18:50:54Z http://irep.iium.edu.my/60912/ Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7) Firus Khan, Al'aina Yuhainis Jalal, Tara Kamal Abdull Rasad, Mohammad Syaiful Bahari Hashim, Ridzwan Abdul Wahab, Ridhwan RM Therapeutics. Pharmacology Purpose: Currently there were many treatments available to treat cancer patients, for instance chemotherapy, radiation therapy, immune therapy, and many more. Unfortunately, advancement in cancer therapy has resulted in increasing numbers of survivors which left to deal with side effects of their treatments. Therefore, discovery of alternative treatment with minimal side effect is crucial. Thus, study on bezoar take place as there were no scientific studies about porcupine bezoar to this date in Malaysia. This lead to the premise that porcupine bezoar could have greater potential for the chemoprevention activities. Methods: MCF-7 cells were treated with bezoar for cell proliferation, cell cycle, apoptosis and qPCR analyses. Cell viability of treated and untreated MCF-7 was examined by Trypan Blue Exclusion method. The cellular morphology of MCF-7 cells was observed by phase contrast microscopy. Flow cytometry analyzed cell apoptosis with annexin V/ propidium iodide (PI) and cell cycle with PI. qPCR was run in detecting gene expression involved in the apoptosis and cell cycle arrest. Result(s): Based on the conducted study and experiments, it is evident that the bezoar treatment in human periodontal ligament cells didn’t show cytotoxicity effect. Upon treated with optimized concentration of bezoar for 24, 48 and 72 hours, it shows inhibition of proliferation by 45.5, 31.25 and 37.1% respectively, significantly apoptosis and cell cycle arrest occurs. Bezoar treatment also induced apoptotic-liked in morphological changes. Upon qPCR analysis, its indicated mcf7 undergo intrinsic pathway of apoptosis and arrest at G1/G0 as gene expression high in cdk2, cyclin D and p21. Conclusion(s): Porcupine bezoar can be alternative treatment for cancer with minimal side effects. Yet, furthermore insights need to be studied. USM 2016-12 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/60912/1/amdi%20.pdf Firus Khan, Al'aina Yuhainis and Jalal, Tara Kamal and Abdull Rasad, Mohammad Syaiful Bahari and Hashim, Ridzwan and Abdul Wahab, Ridhwan (2016) Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7). JOURNAL OF BIOMEDICAL AND CLINICAL SCIENCES, 1 (Special Issue). p. 61. E-ISSN 2550-147X http://apps.amdi.usm.my/journal/index.php/jbcs/issue/viewIssue/Vol%201%2C%20No%201%2C%20Dec%202016%20-%20Special%20Issue/pdf_40
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Firus Khan, Al'aina Yuhainis Jalal, Tara Kamal Abdull Rasad, Mohammad Syaiful Bahari Hashim, Ridzwan Abdul Wahab, Ridhwan Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7)
description	Purpose: Currently there were many treatments available to treat cancer patients, for instance chemotherapy, radiation therapy, immune therapy, and many more. Unfortunately, advancement in cancer therapy has resulted in increasing numbers of survivors which left to deal with side effects of their treatments. Therefore, discovery of alternative treatment with minimal side effect is crucial. Thus, study on bezoar take place as there were no scientific studies about porcupine bezoar to this date in Malaysia. This lead to the premise that porcupine bezoar could have greater potential for the chemoprevention activities. Methods: MCF-7 cells were treated with bezoar for cell proliferation, cell cycle, apoptosis and qPCR analyses. Cell viability of treated and untreated MCF-7 was examined by Trypan Blue Exclusion method. The cellular morphology of MCF-7 cells was observed by phase contrast microscopy. Flow cytometry analyzed cell apoptosis with annexin V/ propidium iodide (PI) and cell cycle with PI. qPCR was run in detecting gene expression involved in the apoptosis and cell cycle arrest. Result(s): Based on the conducted study and experiments, it is evident that the bezoar treatment in human periodontal ligament cells didn’t show cytotoxicity effect. Upon treated with optimized concentration of bezoar for 24, 48 and 72 hours, it shows inhibition of proliferation by 45.5, 31.25 and 37.1% respectively, significantly apoptosis and cell cycle arrest occurs. Bezoar treatment also induced apoptotic-liked in morphological changes. Upon qPCR analysis, its indicated mcf7 undergo intrinsic pathway of apoptosis and arrest at G1/G0 as gene expression high in cdk2, cyclin D and p21. Conclusion(s): Porcupine bezoar can be alternative treatment for cancer with minimal side effects. Yet, furthermore insights need to be studied.
format	Article
author	Firus Khan, Al'aina Yuhainis Jalal, Tara Kamal Abdull Rasad, Mohammad Syaiful Bahari Hashim, Ridzwan Abdul Wahab, Ridhwan
author_facet	Firus Khan, Al'aina Yuhainis Jalal, Tara Kamal Abdull Rasad, Mohammad Syaiful Bahari Hashim, Ridzwan Abdul Wahab, Ridhwan
author_sort	Firus Khan, Al'aina Yuhainis
title	Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7)
title_short	Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7)
title_full	Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7)
title_fullStr	Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7)
title_full_unstemmed	Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7)
title_sort	porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (mcf-7)
publisher	USM
publishDate	2016
url	http://irep.iium.edu.my/60912/ http://irep.iium.edu.my/60912/ http://irep.iium.edu.my/60912/1/amdi%20.pdf
first_indexed	2023-09-18T21:26:23Z
last_indexed	2023-09-18T21:26:23Z
_version_	1777412219285274624

Porcupine bezoar exhibit as anti-cancer activities on human breast adenocarcinoma cells (MCF-7)

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