Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica

The leaves of Tetracera indica are used in traditional medicine for the treatment of diabetes mellitus. This study was aimed at investigating the phytochemical constituents present in T. indica leaves and their activity against digestive enzyme (alphaglucosidase) which may be part of the mechanism o...

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Main Authors:	Muhammad Alhassan, Alhassan, Ahmed, Qamar Uddin, Sarian, Murni Nazira, Khatib, Alfi
Format:	Conference or Workshop Item
Language:	English English
Published:	University Putra Malaysia 2017
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/61007/ http://irep.iium.edu.my/61007/1/Alhassan_IABS%202017_0061.pdf http://irep.iium.edu.my/61007/2/IABS%202017%20PROCEEDINGS.pdf

id	iium-61007
recordtype	eprints
spelling	iium-610072017-12-31T15:16:13Z http://irep.iium.edu.my/61007/ Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica Muhammad Alhassan, Alhassan Ahmed, Qamar Uddin Sarian, Murni Nazira Khatib, Alfi QD Chemistry The leaves of Tetracera indica are used in traditional medicine for the treatment of diabetes mellitus. This study was aimed at investigating the phytochemical constituents present in T. indica leaves and their activity against digestive enzyme (alphaglucosidase) which may be part of the mechanism of the antidiabetic effect of the plant. Crude methanolic extract from the leaves of T. indica which demonstrate significant alpha glucosidase inhibitory activity was separated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Five flavonoids were isolated from the leaves of T. indica, and these compounds were identified as follows: 5,7-dihydroxy-8-methoxyflavone (wogonin), 5,7,8-trihydroxyflavone (norwogonin), quercetin, 5- hydroxy-7-methoxyflavone (tectochrysin) and 41, 3, 5, 7-tetrahydroxyflavone (kaempferol). The isolated compounds show moderate to strong activity against alphaglucosidase enzymes. Insilco studies using molecular docking show the mode of interactions of the compounds with the active site residues and their binding energies. The results of this study reveal that alphaglucosidase inhibition is one of the primary mode of action of T.indica antidiabetic effect. University Putra Malaysia 2017-08-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/61007/1/Alhassan_IABS%202017_0061.pdf application/pdf en http://irep.iium.edu.my/61007/2/IABS%202017%20PROCEEDINGS.pdf Muhammad Alhassan, Alhassan and Ahmed, Qamar Uddin and Sarian, Murni Nazira and Khatib, Alfi (2017) Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica. In: 2nd International Anatomical and Biomedical Scientific Conference 2017, 1-2 Aug 2017, Serdang, Selangor.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Muhammad Alhassan, Alhassan Ahmed, Qamar Uddin Sarian, Murni Nazira Khatib, Alfi Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
description	The leaves of Tetracera indica are used in traditional medicine for the treatment of diabetes mellitus. This study was aimed at investigating the phytochemical constituents present in T. indica leaves and their activity against digestive enzyme (alphaglucosidase) which may be part of the mechanism of the antidiabetic effect of the plant. Crude methanolic extract from the leaves of T. indica which demonstrate significant alpha glucosidase inhibitory activity was separated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Five flavonoids were isolated from the leaves of T. indica, and these compounds were identified as follows: 5,7-dihydroxy-8-methoxyflavone (wogonin), 5,7,8-trihydroxyflavone (norwogonin), quercetin, 5- hydroxy-7-methoxyflavone (tectochrysin) and 41, 3, 5, 7-tetrahydroxyflavone (kaempferol). The isolated compounds show moderate to strong activity against alphaglucosidase enzymes. Insilco studies using molecular docking show the mode of interactions of the compounds with the active site residues and their binding energies. The results of this study reveal that alphaglucosidase inhibition is one of the primary mode of action of T.indica antidiabetic effect.
format	Conference or Workshop Item
author	Muhammad Alhassan, Alhassan Ahmed, Qamar Uddin Sarian, Murni Nazira Khatib, Alfi
author_facet	Muhammad Alhassan, Alhassan Ahmed, Qamar Uddin Sarian, Murni Nazira Khatib, Alfi
author_sort	Muhammad Alhassan, Alhassan
title	Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_short	Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_full	Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_fullStr	Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_full_unstemmed	Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
title_sort	insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica
publisher	University Putra Malaysia
publishDate	2017
url	http://irep.iium.edu.my/61007/ http://irep.iium.edu.my/61007/1/Alhassan_IABS%202017_0061.pdf http://irep.iium.edu.my/61007/2/IABS%202017%20PROCEEDINGS.pdf
first_indexed	2023-09-18T21:26:30Z
last_indexed	2023-09-18T21:26:30Z
_version_	1777412226777350144

Insilico and invitro investigations of alpha-glucosidase inhibitory activity of phenolic constituents of tetracera indica

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