Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach

Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches. Design and method: Initially, the fruit was extracted by soaking in different so...

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Main Authors:	Perumal, Vikneswari, Ahmed, Qamar Uddin, Khatib, Alfi, Uzir, Bisha Fathamah, Murugesu, Suganya
Format:	Conference or Workshop Item
Language:	English English
Published:	University Putra Malaysia 2017
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/61010/ http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf

id	iium-61010
recordtype	eprints
spelling	iium-610102017-12-31T14:41:30Z http://irep.iium.edu.my/61010/ Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya QD Chemistry Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches. Design and method: Initially, the fruit was extracted by soaking in different solvents with different concentrations of ethanol in water (0, 20, 40, 60, 80, 100%, v/v). Then, the extracts were tested for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. LC-MS based metabolomics approach correlated with multivariate data analysis was applied to profile the bioactive compounds present in the extract. Results The 80% ethanol extract showed a high inhibitory activity on 1, 1-diphenyl-2 picrylhydrazyl (DPPH) radical and high ferric reducing antioxidant power, LC-MS based metabolomics approaches helped to identify several antioxidants in this extract such as ascorbic acid, margarolic acid, brevifolincarboxylic acid, quercetin 3-O-glycoside, kuguacin H, cucurbitacin E, 3-malonylmomordicin I, goyaglycoside G. Conclusion: The results of the present study shows possible antioxidants from M. charantia fruit can be identified Keywords: Momordica charantia, LCMS, Metabolomics, antioxidant, multivariate data analysis. University Putra Malaysia 2017-08-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf application/pdf en http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf Perumal, Vikneswari and Ahmed, Qamar Uddin and Khatib, Alfi and Uzir, Bisha Fathamah and Murugesu, Suganya (2017) Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach. In: 2nd International Anatomical and Biomedical Scientific Conference 2017, 1-2 Aug 2017, Serdang, Selangor.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach
description	Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches. Design and method: Initially, the fruit was extracted by soaking in different solvents with different concentrations of ethanol in water (0, 20, 40, 60, 80, 100%, v/v). Then, the extracts were tested for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. LC-MS based metabolomics approach correlated with multivariate data analysis was applied to profile the bioactive compounds present in the extract. Results The 80% ethanol extract showed a high inhibitory activity on 1, 1-diphenyl-2 picrylhydrazyl (DPPH) radical and high ferric reducing antioxidant power, LC-MS based metabolomics approaches helped to identify several antioxidants in this extract such as ascorbic acid, margarolic acid, brevifolincarboxylic acid, quercetin 3-O-glycoside, kuguacin H, cucurbitacin E, 3-malonylmomordicin I, goyaglycoside G. Conclusion: The results of the present study shows possible antioxidants from M. charantia fruit can be identified Keywords: Momordica charantia, LCMS, Metabolomics, antioxidant, multivariate data analysis.
format	Conference or Workshop Item
author	Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya
author_facet	Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya
author_sort	Perumal, Vikneswari
title	Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach
title_short	Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach
title_full	Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach
title_fullStr	Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach
title_full_unstemmed	Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach
title_sort	evaluation of antioxidants activity of momordica charantia using lc-ms metabolomics approach
publisher	University Putra Malaysia
publishDate	2017
url	http://irep.iium.edu.my/61010/ http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf
first_indexed	2023-09-18T21:26:30Z
last_indexed	2023-09-18T21:26:30Z
_version_	1777412227224043520

Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach

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