Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach
Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches. Design and method: Initially, the fruit was extracted by soaking in different so...
Main Authors: | , , , , |
---|---|
Format: | Conference or Workshop Item |
Language: | English English |
Published: |
University Putra Malaysia
2017
|
Subjects: | |
Online Access: | http://irep.iium.edu.my/61010/ http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf |
id |
iium-61010 |
---|---|
recordtype |
eprints |
spelling |
iium-610102017-12-31T14:41:30Z http://irep.iium.edu.my/61010/ Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya QD Chemistry Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches. Design and method: Initially, the fruit was extracted by soaking in different solvents with different concentrations of ethanol in water (0, 20, 40, 60, 80, 100%, v/v). Then, the extracts were tested for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. LC-MS based metabolomics approach correlated with multivariate data analysis was applied to profile the bioactive compounds present in the extract. Results The 80% ethanol extract showed a high inhibitory activity on 1, 1-diphenyl-2 picrylhydrazyl (DPPH) radical and high ferric reducing antioxidant power, LC-MS based metabolomics approaches helped to identify several antioxidants in this extract such as ascorbic acid, margarolic acid, brevifolincarboxylic acid, quercetin 3-O-glycoside, kuguacin H, cucurbitacin E, 3-malonylmomordicin I, goyaglycoside G. Conclusion: The results of the present study shows possible antioxidants from M. charantia fruit can be identified Keywords: Momordica charantia, LCMS, Metabolomics, antioxidant, multivariate data analysis. University Putra Malaysia 2017-08-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf application/pdf en http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf Perumal, Vikneswari and Ahmed, Qamar Uddin and Khatib, Alfi and Uzir, Bisha Fathamah and Murugesu, Suganya (2017) Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach. In: 2nd International Anatomical and Biomedical Scientific Conference 2017, 1-2 Aug 2017, Serdang, Selangor. |
repository_type |
Digital Repository |
institution_category |
Local University |
institution |
International Islamic University Malaysia |
building |
IIUM Repository |
collection |
Online Access |
language |
English English |
topic |
QD Chemistry |
spellingShingle |
QD Chemistry Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
description |
Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches.
Design and method: Initially, the fruit was extracted by soaking in different solvents with different concentrations of ethanol in water (0, 20, 40, 60, 80, 100%, v/v). Then, the extracts were tested for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. LC-MS based metabolomics approach correlated with multivariate data analysis was applied to profile the bioactive compounds present in the extract.
Results The 80% ethanol extract showed a high inhibitory activity on 1, 1-diphenyl-2 picrylhydrazyl (DPPH) radical and high ferric reducing antioxidant power, LC-MS based metabolomics approaches helped to identify several antioxidants in this extract such as ascorbic acid, margarolic acid, brevifolincarboxylic acid, quercetin 3-O-glycoside, kuguacin H, cucurbitacin E, 3-malonylmomordicin I, goyaglycoside G.
Conclusion: The results of the present study shows possible antioxidants from M. charantia fruit can be identified
Keywords: Momordica charantia, LCMS, Metabolomics, antioxidant, multivariate data analysis. |
format |
Conference or Workshop Item |
author |
Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya |
author_facet |
Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya |
author_sort |
Perumal, Vikneswari |
title |
Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
title_short |
Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
title_full |
Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
title_fullStr |
Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
title_full_unstemmed |
Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
title_sort |
evaluation of antioxidants activity of momordica charantia using lc-ms metabolomics approach |
publisher |
University Putra Malaysia |
publishDate |
2017 |
url |
http://irep.iium.edu.my/61010/ http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf |
first_indexed |
2023-09-18T21:26:30Z |
last_indexed |
2023-09-18T21:26:30Z |
_version_ |
1777412227224043520 |