Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts

Objectives: The present study was designed to investigate the antidiabetic and antioxidant activities of the different solvents extracts of Clinacanthus nutans (Burm. F) Lindau leaves through different bioassays as well as to identify the functional group(s) responsible for the particular bioactivit...

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Main Authors:	Khatib, Alfi, Ahmed, Qamar Uddin, Uzir, Bisha Fathamah
Format:	Conference or Workshop Item
Language:	English English
Published:	UPM Press 2017
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/61011/ http://irep.iium.edu.my/61011/1/SUGA_IABS%200060-%20SUGA-%20ABSTRACT.pdf http://irep.iium.edu.my/61011/2/IABS%202017%20PROCEEDINGS.pdf

id	iium-61011
recordtype	eprints
spelling	iium-610112017-12-31T14:33:34Z http://irep.iium.edu.my/61011/ Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts Khatib, Alfi Ahmed, Qamar Uddin Uzir, Bisha Fathamah QD Chemistry Objectives: The present study was designed to investigate the antidiabetic and antioxidant activities of the different solvents extracts of Clinacanthus nutans (Burm. F) Lindau leaves through different bioassays as well as to identify the functional group(s) responsible for the particular bioactivity through Fourier transform infrared (FT-IR) spectroscopy. Design and method: Mature leaves of C. nutans were collected, oven dried at 40 °C, powdered, and extracted in 80% hydro-methanol to obtain crude extract. This extract was then subjected to liquid to liquid partition to obtain hexane, ethyl acetate, butanol and aqueous extracts. All the extracts were then analysed for their bioactivity through various bioassays which include anti-oxidant (DPPH and FRAP) assay, xanthine oxidase and α-glucosidase inhibitory assays. FTIR analyses for the qualitative identification of bioactive compounds was then carried out to all the extracts. Results: Bioactivity analyses of the plant’s leaves extracts on anti-oxidant (DPPH and FRAP) assay, xanthine oxidase and α-glucosidase inhibitory assays revealed that the hexane extract was highly active against α-glucosidase. Meanwhile ethyl acetate exhibited average activity in DPPH scavenging and xanthine oxidase inhibitory assays. Highest FRAP value was exhibited by ethyl acetate extract compared to others. Interestingly, FT-IR spectra analyses of each extract confirmed the presence of different functional groups that may have contributed to the various biological activity. Conclusion: The results of the present study produced the FTIR spectrum profile for the vulnerable medicinally important plant C. nutans (Burm. F) Lindau that further confirms its medicinal values. Hence, C. nutans leaves extract is medicinally potent that may serve essentially in the development of new pharmaceuticals for natural plant-based medicine. Keywords: Clinacanthus nutans (Burm. F) Lindau, DPPH, FRAP, xanthine oxidase, α-glucosidase, FTIR, active principles UPM Press 2017-08-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/61011/1/SUGA_IABS%200060-%20SUGA-%20ABSTRACT.pdf application/pdf en http://irep.iium.edu.my/61011/2/IABS%202017%20PROCEEDINGS.pdf Khatib, Alfi and Ahmed, Qamar Uddin and Uzir, Bisha Fathamah (2017) Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts. In: 2nd International Anatomical and Biomedical Scientific Conference 2017, 1-2 Aug 2017, Serdang, Selangor.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Khatib, Alfi Ahmed, Qamar Uddin Uzir, Bisha Fathamah Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts
description	Objectives: The present study was designed to investigate the antidiabetic and antioxidant activities of the different solvents extracts of Clinacanthus nutans (Burm. F) Lindau leaves through different bioassays as well as to identify the functional group(s) responsible for the particular bioactivity through Fourier transform infrared (FT-IR) spectroscopy. Design and method: Mature leaves of C. nutans were collected, oven dried at 40 °C, powdered, and extracted in 80% hydro-methanol to obtain crude extract. This extract was then subjected to liquid to liquid partition to obtain hexane, ethyl acetate, butanol and aqueous extracts. All the extracts were then analysed for their bioactivity through various bioassays which include anti-oxidant (DPPH and FRAP) assay, xanthine oxidase and α-glucosidase inhibitory assays. FTIR analyses for the qualitative identification of bioactive compounds was then carried out to all the extracts. Results: Bioactivity analyses of the plant’s leaves extracts on anti-oxidant (DPPH and FRAP) assay, xanthine oxidase and α-glucosidase inhibitory assays revealed that the hexane extract was highly active against α-glucosidase. Meanwhile ethyl acetate exhibited average activity in DPPH scavenging and xanthine oxidase inhibitory assays. Highest FRAP value was exhibited by ethyl acetate extract compared to others. Interestingly, FT-IR spectra analyses of each extract confirmed the presence of different functional groups that may have contributed to the various biological activity. Conclusion: The results of the present study produced the FTIR spectrum profile for the vulnerable medicinally important plant C. nutans (Burm. F) Lindau that further confirms its medicinal values. Hence, C. nutans leaves extract is medicinally potent that may serve essentially in the development of new pharmaceuticals for natural plant-based medicine. Keywords: Clinacanthus nutans (Burm. F) Lindau, DPPH, FRAP, xanthine oxidase, α-glucosidase, FTIR, active principles
format	Conference or Workshop Item
author	Khatib, Alfi Ahmed, Qamar Uddin Uzir, Bisha Fathamah
author_facet	Khatib, Alfi Ahmed, Qamar Uddin Uzir, Bisha Fathamah
author_sort	Khatib, Alfi
title	Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts
title_short	Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts
title_full	Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts
title_fullStr	Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts
title_full_unstemmed	Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts
title_sort	bioassays activity and ft-ir analysis of clinacanthus nutans (burm f.) lindau leaves extracts
publisher	UPM Press
publishDate	2017
url	http://irep.iium.edu.my/61011/ http://irep.iium.edu.my/61011/1/SUGA_IABS%200060-%20SUGA-%20ABSTRACT.pdf http://irep.iium.edu.my/61011/2/IABS%202017%20PROCEEDINGS.pdf
first_indexed	2023-09-18T21:26:30Z
last_indexed	2023-09-18T21:26:30Z
_version_	1777412227369795584

Bioassays activity and FT-IR analysis of clinacanthus nutans (Burm F.) lindau leaves extracts

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