Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7)

Objectives/Research Problem: Breast cancer is a major health problem in Malaysia and the world. It is the commonest malignancy in women and the second leading cause of cancer deaths. There are many conventional treatments available for breast cancer patients, such as chemotherapy, radiation ther...

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Main Authors:	Hassan, Habibah, Razali, Shakirah, Firus Khan, Al'aina, K Jalal, Tara, Salleh, Azil, Khatib, Alfi, Hashim, Ridzwan, Abdul Wahab, Ridhwan
Format:	Article
Language:	English
Published:	International Islamic University Malaysia 2017
Subjects:	SF Animal culture
Online Access:	http://irep.iium.edu.my/61119/ http://irep.iium.edu.my/61119/ http://irep.iium.edu.my/61119/1/IJAHS%20ABSTRACT%20HABIBAH%20HASSAN.pdf

id	iium-61119
recordtype	eprints
spelling	iium-611192018-07-11T00:52:48Z http://irep.iium.edu.my/61119/ Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7) Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan SF Animal culture Objectives/Research Problem: Breast cancer is a major health problem in Malaysia and the world. It is the commonest malignancy in women and the second leading cause of cancer deaths. There are many conventional treatments available for breast cancer patients, such as chemotherapy, radiation therapy, immune therapy, hormone therapy and many more. However, drug resistance acquired by cancer cells has led to treatment failure. Alternative treatment with minimum or no side effect is highly demanded. Therefore, the aim of this study was to determine the chemopreventive effect of Luffa aegyptiaca seed extract (LSE) on breast cancer through anti-proliferative effect and its mechanisms on breast cancer cell (MCF-7). It can be evaluated by result of IC₅₀ determination, cell proliferation assay and flow cytometer analysis on apoptosis induction and cell cycle arrest. Materials and Method: MCF-7 cells were seeded at a density of 4.7 x 10⁴ cells/well. Trypan blue exclusion method was used to determine the viable cells using haemocytometer. The IC50 assay was determined against MCF-7 cell at different concentrations of LSE administration. The morphological changes of MCF-7 cells also were observed. MCF-7 cell and 3T3-L1 cell were treated with LSE at IC50 to evaluate the cell proliferation assay and cytotoxicity assay, respectively. The induction of apoptosis and alteration on cell cycle regulation will be assessed by flow cytometer. Results and Discussion: LSE showed the growth inhibitory effect with IC₅₀ value at 0.0625 mg/ml after 72 hours exposure. Morphological changes displaying apoptosis also had been observed upon treatment with LSE at IC50. Moreover, LSE showed no toxicity effect on normal cells (3T3-L1). Thus proved the potential chemopreventive agent of the extracts on MCF-7. The results for flow cytometric analysis are yet to be determined as the works are still in progress. Conclusion: Overall, the data collected provided new insight of using the Luffa aegyptiaca seed which can be used as chemopreventive agents on MCF-7. KEYWORDS: Cancer Chemoprevention, Breast Cancer, Luffa aegyptiaca, Anti-Proliferative Effect, IC₅₀ Determination, Apoptosis, Cell Cycle, MCF-7 International Islamic University Malaysia 2017-09-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61119/1/IJAHS%20ABSTRACT%20HABIBAH%20HASSAN.pdf Hassan, Habibah and Razali, Shakirah and Firus Khan, Al'aina and K Jalal, Tara and Salleh, Azil and Khatib, Alfi and Hashim, Ridzwan and Abdul Wahab, Ridhwan (2017) Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7). International Journal of Allied Health Sciences, 1 (1). p. 13. E-ISSN 2600-8491 http://journals.iium.edu.my/ijahs/index.php/IJAHS
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SF Animal culture
spellingShingle	SF Animal culture Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7)
description	Objectives/Research Problem: Breast cancer is a major health problem in Malaysia and the world. It is the commonest malignancy in women and the second leading cause of cancer deaths. There are many conventional treatments available for breast cancer patients, such as chemotherapy, radiation therapy, immune therapy, hormone therapy and many more. However, drug resistance acquired by cancer cells has led to treatment failure. Alternative treatment with minimum or no side effect is highly demanded. Therefore, the aim of this study was to determine the chemopreventive effect of Luffa aegyptiaca seed extract (LSE) on breast cancer through anti-proliferative effect and its mechanisms on breast cancer cell (MCF-7). It can be evaluated by result of IC₅₀ determination, cell proliferation assay and flow cytometer analysis on apoptosis induction and cell cycle arrest. Materials and Method: MCF-7 cells were seeded at a density of 4.7 x 10⁴ cells/well. Trypan blue exclusion method was used to determine the viable cells using haemocytometer. The IC50 assay was determined against MCF-7 cell at different concentrations of LSE administration. The morphological changes of MCF-7 cells also were observed. MCF-7 cell and 3T3-L1 cell were treated with LSE at IC50 to evaluate the cell proliferation assay and cytotoxicity assay, respectively. The induction of apoptosis and alteration on cell cycle regulation will be assessed by flow cytometer. Results and Discussion: LSE showed the growth inhibitory effect with IC₅₀ value at 0.0625 mg/ml after 72 hours exposure. Morphological changes displaying apoptosis also had been observed upon treatment with LSE at IC50. Moreover, LSE showed no toxicity effect on normal cells (3T3-L1). Thus proved the potential chemopreventive agent of the extracts on MCF-7. The results for flow cytometric analysis are yet to be determined as the works are still in progress. Conclusion: Overall, the data collected provided new insight of using the Luffa aegyptiaca seed which can be used as chemopreventive agents on MCF-7. KEYWORDS: Cancer Chemoprevention, Breast Cancer, Luffa aegyptiaca, Anti-Proliferative Effect, IC₅₀ Determination, Apoptosis, Cell Cycle, MCF-7
format	Article
author	Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan
author_facet	Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan
author_sort	Hassan, Habibah
title	Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7)
title_short	Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7)
title_full	Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7)
title_fullStr	Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7)
title_full_unstemmed	Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7)
title_sort	cancer chemoprevention study of luffa aegyptiaca seed extract on human breast cancer cell lines (mcf-7)
publisher	International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/61119/ http://irep.iium.edu.my/61119/ http://irep.iium.edu.my/61119/1/IJAHS%20ABSTRACT%20HABIBAH%20HASSAN.pdf
first_indexed	2023-09-18T21:26:41Z
last_indexed	2023-09-18T21:26:41Z
_version_	1777412238141816832

Cancer chemoprevention study of Luffa Aegyptiaca seed extract on human breast cancer cell lines (MCF-7)

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