Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens

Despite the tremendous advancement in medical field, oral infections are still considered as one of the major public health problems suffered by human population; causing a burden to health care system worldwide. Apart from using synthetic antibiotics, anti-bacterial agents from natural resources ar...

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Main Authors:	Fadzir, Ummu Afifah, Mokhtar@Makhtar, Khairani Idah, Mustafa Al-Ahmad, Basma Ezzat, Darnis, Deny Susanti
Format:	Conference or Workshop Item
Language:	English English
Published:	2017
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/61211/ http://irep.iium.edu.my/61211/3/Tentative%20Program%20ICAST%202017.pdf http://irep.iium.edu.my/61211/2/ICAST2017_O-07.pdf

id	iium-61211
recordtype	eprints
spelling	iium-612112018-01-12T04:07:27Z http://irep.iium.edu.my/61211/ Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens Fadzir, Ummu Afifah Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Darnis, Deny Susanti QD Chemistry Despite the tremendous advancement in medical field, oral infections are still considered as one of the major public health problems suffered by human population; causing a burden to health care system worldwide. Apart from using synthetic antibiotics, anti-bacterial agents from natural resources are suggested as an alternative for the treatment of infectious diseases due to lesser side effects imposed. Flaxseed (Linum usitatissimum) has been shown to demonstrate some anti- bacterial properties. However, its effects towards oral pathogens are still limited. Hence, the present study aimed to investigate the anti-bacterial effects of flaxseed extracts against selected oral pathogens. Non-polar and polar extracts of flaxseed; n-hexane, dichloromethane (DCM) and methanol (MeOH), were prepared by sequential extraction using soxhlet extraction method. The anti-bacterial activities of flaxseed extracts with different concentration (5, 7, 10, 12 & 15 μl/disc) were evaluated against three oral pathogens; Streptococcus pyogenes, Streptococcus mutans and Enterococcus faecalis using disc diffusion method. Additionally, extracts which exhibited significant diameter of inhibition zone were screened through GC-MS (n-Hexane & DCM) or LC-MS (MeOH) to detect the presence of bioactive compound. Polar extract (methanolic extract) demonstrated significant antibacterial activity (p< 0.05) against all tested oral pathogens which is comparable with the zone of inhibition exhibited by penicillin (positive control). In contrast, n-hexane and DCM extracts demonstrated variable anti-bacterial activities. GC-MS screening showed the presence of some fatty acids in n-hexane and DCM extracts. And in LC-MS screening demonstrated the presence of linolenic acid, secoisolariciresinol diglycoside, (-)-secoisolariciresinol-4-O-β-D-glucopyranoside E-p-coumaric acid, and flavone,5,7-dihydroxy-4′-O-α-D-glucoside which may have contributed greatly to the inhibitory activity of the methanolic plant extract. From the study, it can be concluded that methanolic extract of L. usitatissimum exhibited the best inhibitory activity at 15 μl/disc on S. pyogenes, S. mutans and E. faecalis. 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/61211/3/Tentative%20Program%20ICAST%202017.pdf application/pdf en http://irep.iium.edu.my/61211/2/ICAST2017_O-07.pdf Fadzir, Ummu Afifah and Mokhtar@Makhtar, Khairani Idah and Mustafa Al-Ahmad, Basma Ezzat and Darnis, Deny Susanti (2017) Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens. In: 6th International Conference on Advancement in Science and Technology (iCAST 2017), 8th-9th August 2017, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Fadzir, Ummu Afifah Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Darnis, Deny Susanti Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens
description	Despite the tremendous advancement in medical field, oral infections are still considered as one of the major public health problems suffered by human population; causing a burden to health care system worldwide. Apart from using synthetic antibiotics, anti-bacterial agents from natural resources are suggested as an alternative for the treatment of infectious diseases due to lesser side effects imposed. Flaxseed (Linum usitatissimum) has been shown to demonstrate some anti- bacterial properties. However, its effects towards oral pathogens are still limited. Hence, the present study aimed to investigate the anti-bacterial effects of flaxseed extracts against selected oral pathogens. Non-polar and polar extracts of flaxseed; n-hexane, dichloromethane (DCM) and methanol (MeOH), were prepared by sequential extraction using soxhlet extraction method. The anti-bacterial activities of flaxseed extracts with different concentration (5, 7, 10, 12 & 15 μl/disc) were evaluated against three oral pathogens; Streptococcus pyogenes, Streptococcus mutans and Enterococcus faecalis using disc diffusion method. Additionally, extracts which exhibited significant diameter of inhibition zone were screened through GC-MS (n-Hexane & DCM) or LC-MS (MeOH) to detect the presence of bioactive compound. Polar extract (methanolic extract) demonstrated significant antibacterial activity (p< 0.05) against all tested oral pathogens which is comparable with the zone of inhibition exhibited by penicillin (positive control). In contrast, n-hexane and DCM extracts demonstrated variable anti-bacterial activities. GC-MS screening showed the presence of some fatty acids in n-hexane and DCM extracts. And in LC-MS screening demonstrated the presence of linolenic acid, secoisolariciresinol diglycoside, (-)-secoisolariciresinol-4-O-β-D-glucopyranoside E-p-coumaric acid, and flavone,5,7-dihydroxy-4′-O-α-D-glucoside which may have contributed greatly to the inhibitory activity of the methanolic plant extract. From the study, it can be concluded that methanolic extract of L. usitatissimum exhibited the best inhibitory activity at 15 μl/disc on S. pyogenes, S. mutans and E. faecalis.
format	Conference or Workshop Item
author	Fadzir, Ummu Afifah Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Darnis, Deny Susanti
author_facet	Fadzir, Ummu Afifah Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Darnis, Deny Susanti
author_sort	Fadzir, Ummu Afifah
title	Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens
title_short	Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens
title_full	Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens
title_fullStr	Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens
title_full_unstemmed	Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens
title_sort	identification and detection of bioactive compounds in linum usitatissimum (flaxseed) and its antibacterial properties against selected oral pathogens
publishDate	2017
url	http://irep.iium.edu.my/61211/ http://irep.iium.edu.my/61211/3/Tentative%20Program%20ICAST%202017.pdf http://irep.iium.edu.my/61211/2/ICAST2017_O-07.pdf
first_indexed	2023-09-18T21:26:48Z
last_indexed	2023-09-18T21:26:48Z
_version_	1777412246303932416

Identification and detection of bioactive compounds in Linum usitatissimum (Flaxseed) and its antibacterial properties against selected oral pathogens

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