Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats

Objectives/Research Problem: hite adipose tissue is not just an energy storage depot but now it is recognized to be an active organ in energy homeostasis and physiological functions. It is known to secrete a variety of bioactive proteins known as adipokines. Among these adipokines, adiponectin is re...

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Main Authors:	Azahari, Nuraniza, Khattak, Muhammad Muzaffar Ali Khan, Mohd Shukri, Nor Azwani
Format:	Article
Language:	English
Published:	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2017
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/61712/ http://irep.iium.edu.my/61712/ http://irep.iium.edu.my/61712/1/61712_Antihyperglycemic%20activities%20of%20purified%20protein.pdf

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spelling	iium-617122019-09-03T02:19:32Z http://irep.iium.edu.my/61712/ Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani TX341 Nutrition. Foods and food supply Objectives/Research Problem: hite adipose tissue is not just an energy storage depot but now it is recognized to be an active organ in energy homeostasis and physiological functions. It is known to secrete a variety of bioactive proteins known as adipokines. Among these adipokines, adiponectin is reported to have antidiabetic properties which functions as an insulin sensitizer. The present study was performed to evaluate the effects of purified protein containing adiponectin or insulin alone on blood glucose, blood lipid and histopathological changes in the pancreas of streptozotocin-induced diabetic rats.Materials and Method: In this study, thirty male adult Sprague Dawley rats (Rattus norvegicus) weighing 150-250 grams (8-10 weeks) were randomly divided into six experimental groups A, B, C, D, E and F. Hyperglycemia was induced by a single intraperitoneal injection of streptozotocin (STZ) at rate of 70 mg/kg body weight. Seven days after, the confirmation of hyperglycemia by using a glucometer (Accu-Chek Active) and compatible glucose test strips, Groups A, B and C were treated with 30 mg/kg body weight of purified protein containing adiponectin from chicken, beef and lamb adipose tissues, respectively. Group E was treated with 2 I U/kg body weight insulin. Groups A, B, and C served as hyperglycemia and group F was a normal control and received 1 ml/kg body weight citrate buffer. After 14 days of treatment, the animals were sacrificed, the blood was analysed and the pancreas was processed for histological staining. Data obtained were expressed as means of four replicates.Results and Discussion: Findings showed that STZ-induced diabetes induced hyperglycemia and histopathological changes in the pancreas of rats.Conclusion: Treatment with the purified protein containing adiponectin from abdominal adipose tissues of halal meat resulted in reduction in hyperglycemia and regeneration of beta cells. Kulliyyah of Allied Health Sciences, International Islamic University Malaysia 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61712/1/61712_Antihyperglycemic%20activities%20of%20purified%20protein.pdf Azahari, Nuraniza and Khattak, Muhammad Muzaffar Ali Khan and Mohd Shukri, Nor Azwani (2017) Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats. International Journal of Allied Health Sciences, 1. p. 47. E-ISSN 2600-8491 http://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/91
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats
description	Objectives/Research Problem: hite adipose tissue is not just an energy storage depot but now it is recognized to be an active organ in energy homeostasis and physiological functions. It is known to secrete a variety of bioactive proteins known as adipokines. Among these adipokines, adiponectin is reported to have antidiabetic properties which functions as an insulin sensitizer. The present study was performed to evaluate the effects of purified protein containing adiponectin or insulin alone on blood glucose, blood lipid and histopathological changes in the pancreas of streptozotocin-induced diabetic rats.Materials and Method: In this study, thirty male adult Sprague Dawley rats (Rattus norvegicus) weighing 150-250 grams (8-10 weeks) were randomly divided into six experimental groups A, B, C, D, E and F. Hyperglycemia was induced by a single intraperitoneal injection of streptozotocin (STZ) at rate of 70 mg/kg body weight. Seven days after, the confirmation of hyperglycemia by using a glucometer (Accu-Chek Active) and compatible glucose test strips, Groups A, B and C were treated with 30 mg/kg body weight of purified protein containing adiponectin from chicken, beef and lamb adipose tissues, respectively. Group E was treated with 2 I U/kg body weight insulin. Groups A, B, and C served as hyperglycemia and group F was a normal control and received 1 ml/kg body weight citrate buffer. After 14 days of treatment, the animals were sacrificed, the blood was analysed and the pancreas was processed for histological staining. Data obtained were expressed as means of four replicates.Results and Discussion: Findings showed that STZ-induced diabetes induced hyperglycemia and histopathological changes in the pancreas of rats.Conclusion: Treatment with the purified protein containing adiponectin from abdominal adipose tissues of halal meat resulted in reduction in hyperglycemia and regeneration of beta cells.
format	Article
author	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani
author_facet	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Mohd Shukri, Nor Azwani
author_sort	Azahari, Nuraniza
title	Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats
title_short	Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats
title_full	Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats
title_fullStr	Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats
title_full_unstemmed	Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats
title_sort	antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of halal meat on the streptozotocin-induced diabetic rats
publisher	Kulliyyah of Allied Health Sciences, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/61712/ http://irep.iium.edu.my/61712/ http://irep.iium.edu.my/61712/1/61712_Antihyperglycemic%20activities%20of%20purified%20protein.pdf
first_indexed	2023-09-18T21:27:30Z
last_indexed	2023-09-18T21:27:30Z
_version_	1777412290392358912

Antihyperglycemic activities of purified protein containing adiponectin from abdominal adipose tissues of Halal meat on the streptozotocin-induced diabetic rats

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