Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves
INTRODUCTION: Hydroxychavicol is commonly extracted from Piper betle leaves using conventional solvent extraction. However, there is a modern green extraction technique using subcritical water to extract hydroxychavicol at a high temperature between 100 and 374℃ and a high pressure. It is used due t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
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Malaysian Pharmaceutical Society
2017
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| Online Access: | http://irep.iium.edu.my/61758/ http://irep.iium.edu.my/61758/ http://irep.iium.edu.my/61758/18/61758-Subcritical%20water%20extraction%20as%20a%20modern.pdf http://irep.iium.edu.my/61758/3/ORAL%20PRESENTATION%20MPS%20NADZIRA%20PDF.pdf |
| Summary: | INTRODUCTION: Hydroxychavicol is commonly extracted from Piper betle leaves using conventional solvent extraction. However, there is a modern green extraction technique using subcritical water to extract hydroxychavicol at a high temperature between 100 and 374℃ and a high pressure. It is used due to cost-effectiveness, time-saving, greater extraction yields and environmental friendly.OBJECTIVES: To determine optimum conditions for subcritical water extraction of hydroxychavicol and to compare hydroxychavicol yield between conventional solvent extraction and subcritical water extraction techniques.METHODS: Subcritical water extraction of hydroxychavicol was carried out at different temperatures ranging from 80 to 180℃. Then, it was conducted at different static extraction time interval of 10, 30, 60 and 90 minutes with total extraction time of 180 minutes. The hydroxychavicol content was analyzed by using a validated high performance liquid chromatography (HPLC) method. The HPLC method was validated for linearity, specificity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ).RESULTS: The highest amount of hydroxychavicol (11.05%) was extracted from Piper betle leaves using subcritical water extraction at 100℃ with static extraction time interval of 60 minutes. Subcritical water extraction showed significantly greater hydroxychavicol yield of 11.05% compared to solvent extraction of 7.78% (P=0.04). The HPLC method showed a good linearity (r2>0.999), satisfactory accuracy (percentage difference 0.1 to 1.33%), precision (RSD 0.11 to 1.44%) with low LOD (0.13µg/mL) and LOQ (0.43µg/mL).CONCLUSION: Subcritical water extraction has potential to be an alternative extraction technique of hydroxychavicol from Piper betle leaves. |
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