Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves

INTRODUCTION: Hydroxychavicol is commonly extracted from Piper betle leaves using conventional solvent extraction. However, there is a modern green extraction technique using subcritical water to extract hydroxychavicol at a high temperature between 100 and 374℃ and a high pressure. It is used due t...

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Main Authors:	Mohd Hanif, Nadzirah, Sarker, Md. Zaidul Islam, Ab. Hadi, Hazrina
Format:	Article
Language:	English English
Published:	Malaysian Pharmaceutical Society 2017
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/61758/ http://irep.iium.edu.my/61758/ http://irep.iium.edu.my/61758/18/61758-Subcritical%20water%20extraction%20as%20a%20modern.pdf http://irep.iium.edu.my/61758/3/ORAL%20PRESENTATION%20MPS%20NADZIRA%20PDF.pdf

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spelling	iium-617582018-02-06T03:25:05Z http://irep.iium.edu.my/61758/ Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves Mohd Hanif, Nadzirah Sarker, Md. Zaidul Islam Ab. Hadi, Hazrina RS Pharmacy and materia medica INTRODUCTION: Hydroxychavicol is commonly extracted from Piper betle leaves using conventional solvent extraction. However, there is a modern green extraction technique using subcritical water to extract hydroxychavicol at a high temperature between 100 and 374℃ and a high pressure. It is used due to cost-effectiveness, time-saving, greater extraction yields and environmental friendly.OBJECTIVES: To determine optimum conditions for subcritical water extraction of hydroxychavicol and to compare hydroxychavicol yield between conventional solvent extraction and subcritical water extraction techniques.METHODS: Subcritical water extraction of hydroxychavicol was carried out at different temperatures ranging from 80 to 180℃. Then, it was conducted at different static extraction time interval of 10, 30, 60 and 90 minutes with total extraction time of 180 minutes. The hydroxychavicol content was analyzed by using a validated high performance liquid chromatography (HPLC) method. The HPLC method was validated for linearity, specificity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ).RESULTS: The highest amount of hydroxychavicol (11.05%) was extracted from Piper betle leaves using subcritical water extraction at 100℃ with static extraction time interval of 60 minutes. Subcritical water extraction showed significantly greater hydroxychavicol yield of 11.05% compared to solvent extraction of 7.78% (P=0.04). The HPLC method showed a good linearity (r2>0.999), satisfactory accuracy (percentage difference 0.1 to 1.33%), precision (RSD 0.11 to 1.44%) with low LOD (0.13µg/mL) and LOQ (0.43µg/mL).CONCLUSION: Subcritical water extraction has potential to be an alternative extraction technique of hydroxychavicol from Piper betle leaves. Malaysian Pharmaceutical Society 2017-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61758/18/61758-Subcritical%20water%20extraction%20as%20a%20modern.pdf application/pdf en http://irep.iium.edu.my/61758/3/ORAL%20PRESENTATION%20MPS%20NADZIRA%20PDF.pdf Mohd Hanif, Nadzirah and Sarker, Md. Zaidul Islam and Ab. Hadi, Hazrina (2017) Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves. Malaysian Journal of Pharmacy, 3 (1 (Supplement)). p. 24. ISSN 1675-3666 http://www.mps.org.my/index.cfm?&menuid=146
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohd Hanif, Nadzirah Sarker, Md. Zaidul Islam Ab. Hadi, Hazrina Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves
description	INTRODUCTION: Hydroxychavicol is commonly extracted from Piper betle leaves using conventional solvent extraction. However, there is a modern green extraction technique using subcritical water to extract hydroxychavicol at a high temperature between 100 and 374℃ and a high pressure. It is used due to cost-effectiveness, time-saving, greater extraction yields and environmental friendly.OBJECTIVES: To determine optimum conditions for subcritical water extraction of hydroxychavicol and to compare hydroxychavicol yield between conventional solvent extraction and subcritical water extraction techniques.METHODS: Subcritical water extraction of hydroxychavicol was carried out at different temperatures ranging from 80 to 180℃. Then, it was conducted at different static extraction time interval of 10, 30, 60 and 90 minutes with total extraction time of 180 minutes. The hydroxychavicol content was analyzed by using a validated high performance liquid chromatography (HPLC) method. The HPLC method was validated for linearity, specificity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ).RESULTS: The highest amount of hydroxychavicol (11.05%) was extracted from Piper betle leaves using subcritical water extraction at 100℃ with static extraction time interval of 60 minutes. Subcritical water extraction showed significantly greater hydroxychavicol yield of 11.05% compared to solvent extraction of 7.78% (P=0.04). The HPLC method showed a good linearity (r2>0.999), satisfactory accuracy (percentage difference 0.1 to 1.33%), precision (RSD 0.11 to 1.44%) with low LOD (0.13µg/mL) and LOQ (0.43µg/mL).CONCLUSION: Subcritical water extraction has potential to be an alternative extraction technique of hydroxychavicol from Piper betle leaves.
format	Article
author	Mohd Hanif, Nadzirah Sarker, Md. Zaidul Islam Ab. Hadi, Hazrina
author_facet	Mohd Hanif, Nadzirah Sarker, Md. Zaidul Islam Ab. Hadi, Hazrina
author_sort	Mohd Hanif, Nadzirah
title	Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves
title_short	Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves
title_full	Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves
title_fullStr	Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves
title_full_unstemmed	Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves
title_sort	subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves
publisher	Malaysian Pharmaceutical Society
publishDate	2017
url	http://irep.iium.edu.my/61758/ http://irep.iium.edu.my/61758/ http://irep.iium.edu.my/61758/18/61758-Subcritical%20water%20extraction%20as%20a%20modern.pdf http://irep.iium.edu.my/61758/3/ORAL%20PRESENTATION%20MPS%20NADZIRA%20PDF.pdf
first_indexed	2023-09-18T21:27:35Z
last_indexed	2023-09-18T21:27:35Z
_version_	1777412294789038080

Subcritical water extraction as a modern green extraction technique of hydroxychavicol from piper betle linn. leaves

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