Invitro activity of T4 bacteriophage on uropathogenic e. Coli

Bacteriophage is a virus that infects bacteria and can kill a bacterial cell or integrate its nucleic acid (DNA or RNA) into the host bacterial cell. One of the phage important medical applications is using it as an alternative approach to treatment of infections by resistant pathogenic bacteria. Th...

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Main Authors: Almanseekaana, Limes Husam, Mustafa Mahmoud, Mohammed Imad Al-Deen
Format: Book
Language:English
Published: IIUM Press 2017
Subjects:
Online Access:http://irep.iium.edu.my/61948/
http://irep.iium.edu.my/61948/1/61948_Invitro%20activity%20of%20T4%20bacteriophage.pdf
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recordtype eprints
spelling iium-619482019-10-22T01:47:32Z http://irep.iium.edu.my/61948/ Invitro activity of T4 bacteriophage on uropathogenic e. Coli Almanseekaana, Limes Husam Mustafa Mahmoud, Mohammed Imad Al-Deen R Medicine (General) Bacteriophage is a virus that infects bacteria and can kill a bacterial cell or integrate its nucleic acid (DNA or RNA) into the host bacterial cell. One of the phage important medical applications is using it as an alternative approach to treatment of infections by resistant pathogenic bacteria. This book aims to review the literature concerning the use of bacteriophages for the treatment of clinical bacterial infections and emphasizing our own research findings on the susceptibility of drug resistant uropathogenic E. coli towards the T4 phage. The study involved collection of resistant uropathogenic E. coli (UPEC) toward (Ceftazidime, Gentamicin, Trimethoprim/Sulfamethoxazole, Ampicillin, Amoxicillin with Clavulanic Acid and Ciprofloxacin,) isolates from (Hospital Tengku Ampuan Afzan) HTAA, Kuantan, Pahang during a 4 months period (from 01/September/2015 to 31/December/2015). Re-identification of UPEC isolates was done by scientifically approved conventional diagnostic methods. To store isolates for further laboratory procedures, approved long term and short term bacterial storage methods were followed. All UPEC isolates were checked for antimicrobial susceptibility test by the Kirby & Bauer method and by the extended spectrum beta-lactamase (ESBL) screening test. The quantitation of T4 bacteriophage was done at first by the plaque assay on reference E. coli strain ATCC25922. In the plaque assay for UPEC, serial log dilutions of T4 phage (1 × 10-1, 1 × 10-2, 1 × 10-3 , 1 × 10-4 ,1 × 10-5 , 1 × 10-6 , 1 × 10-7 , 1 × 10-8 , 1 × 10-9 and 1 × 10-10( were incubated with UPEC by using the double agar layer technique. Countable plaques were formed for all UPEC isolates in plates inoculated with phage dilutions of 1 × 10-6 and 1 × 10-7. To determine the phage Minimal Inhibitory Concentration (phage MIC), the microbroth dilution method was performed. The phage MIC for the bacterial isolates was ~1 × 105/mL which is the lowest phage concentration or highest dilution which gave a clear broth (no bacterial growth). Serotyping of UPEC H & O antigens was done by standard agglutination method. The percentage distribution of UPEC serotypes was CAN55 (14%), MSHS94 (6%) and MSHS23a (10%) and unknown serotype (70%). In conclusion, the T4 phage concentration of 1 × 105/mL is regarded as the phage MIC for all the tested UPEC strains showing a lytic effect against UPEC. IIUM Press 2017 Book PeerReviewed application/pdf en http://irep.iium.edu.my/61948/1/61948_Invitro%20activity%20of%20T4%20bacteriophage.pdf Almanseekaana, Limes Husam and Mustafa Mahmoud, Mohammed Imad Al-Deen (2017) Invitro activity of T4 bacteriophage on uropathogenic e. Coli. IIUM Press, Kuala Lumpur, Malaysia. ISBN 978-967-418-733-0
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Almanseekaana, Limes Husam
Mustafa Mahmoud, Mohammed Imad Al-Deen
Invitro activity of T4 bacteriophage on uropathogenic e. Coli
description Bacteriophage is a virus that infects bacteria and can kill a bacterial cell or integrate its nucleic acid (DNA or RNA) into the host bacterial cell. One of the phage important medical applications is using it as an alternative approach to treatment of infections by resistant pathogenic bacteria. This book aims to review the literature concerning the use of bacteriophages for the treatment of clinical bacterial infections and emphasizing our own research findings on the susceptibility of drug resistant uropathogenic E. coli towards the T4 phage. The study involved collection of resistant uropathogenic E. coli (UPEC) toward (Ceftazidime, Gentamicin, Trimethoprim/Sulfamethoxazole, Ampicillin, Amoxicillin with Clavulanic Acid and Ciprofloxacin,) isolates from (Hospital Tengku Ampuan Afzan) HTAA, Kuantan, Pahang during a 4 months period (from 01/September/2015 to 31/December/2015). Re-identification of UPEC isolates was done by scientifically approved conventional diagnostic methods. To store isolates for further laboratory procedures, approved long term and short term bacterial storage methods were followed. All UPEC isolates were checked for antimicrobial susceptibility test by the Kirby & Bauer method and by the extended spectrum beta-lactamase (ESBL) screening test. The quantitation of T4 bacteriophage was done at first by the plaque assay on reference E. coli strain ATCC25922. In the plaque assay for UPEC, serial log dilutions of T4 phage (1 × 10-1, 1 × 10-2, 1 × 10-3 , 1 × 10-4 ,1 × 10-5 , 1 × 10-6 , 1 × 10-7 , 1 × 10-8 , 1 × 10-9 and 1 × 10-10( were incubated with UPEC by using the double agar layer technique. Countable plaques were formed for all UPEC isolates in plates inoculated with phage dilutions of 1 × 10-6 and 1 × 10-7. To determine the phage Minimal Inhibitory Concentration (phage MIC), the microbroth dilution method was performed. The phage MIC for the bacterial isolates was ~1 × 105/mL which is the lowest phage concentration or highest dilution which gave a clear broth (no bacterial growth). Serotyping of UPEC H & O antigens was done by standard agglutination method. The percentage distribution of UPEC serotypes was CAN55 (14%), MSHS94 (6%) and MSHS23a (10%) and unknown serotype (70%). In conclusion, the T4 phage concentration of 1 × 105/mL is regarded as the phage MIC for all the tested UPEC strains showing a lytic effect against UPEC.
format Book
author Almanseekaana, Limes Husam
Mustafa Mahmoud, Mohammed Imad Al-Deen
author_facet Almanseekaana, Limes Husam
Mustafa Mahmoud, Mohammed Imad Al-Deen
author_sort Almanseekaana, Limes Husam
title Invitro activity of T4 bacteriophage on uropathogenic e. Coli
title_short Invitro activity of T4 bacteriophage on uropathogenic e. Coli
title_full Invitro activity of T4 bacteriophage on uropathogenic e. Coli
title_fullStr Invitro activity of T4 bacteriophage on uropathogenic e. Coli
title_full_unstemmed Invitro activity of T4 bacteriophage on uropathogenic e. Coli
title_sort invitro activity of t4 bacteriophage on uropathogenic e. coli
publisher IIUM Press
publishDate 2017
url http://irep.iium.edu.my/61948/
http://irep.iium.edu.my/61948/1/61948_Invitro%20activity%20of%20T4%20bacteriophage.pdf
first_indexed 2023-09-18T21:27:53Z
last_indexed 2023-09-18T21:27:53Z
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