Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications

Polyhydroxyalkanoates (PHAs) are hydrophobic biodegradable thermoplastics that have received considerable attention in biomedical applications due to their biocompatibility, mechanical properties, and biodegradability. In this study, the degradation rate was regulated by optimizing the interactio...

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Main Authors:	Ansari, Nor Faezah, Abdullah, Amirul Al-Ashraf
Format:	Article
Language:	English English
Published:	Springer Verlag 2013
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/1/faezah%20ansari%202013-degradation.pdf http://irep.iium.edu.my/62129/7/62129_Preparation%20and%20characterization_SCOPUS.pdf

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spelling	iium-621292018-04-23T03:42:05Z http://irep.iium.edu.my/62129/ Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications Ansari, Nor Faezah Abdullah, Amirul Al-Ashraf Q Science (General) Polyhydroxyalkanoates (PHAs) are hydrophobic biodegradable thermoplastics that have received considerable attention in biomedical applications due to their biocompatibility, mechanical properties, and biodegradability. In this study, the degradation rate was regulated by optimizing the interaction of parameters that influence the enzymatic degradation of P(3HB) film using response surface methodology (RSM). The RSM model was experimentally validated yielding a maximum 21 % weight loss, which represents onefold increment in percentage weight loss in comparison with the conventional method. By using the optimized condition, the enzymatic degradation by an extracellular PHA depolymerase from Acidovorax sp. DP5 was studied at 37 °C and pH 9.0 on different types of PHA films with various monomer compositions. Surface modification of scaffold was employed using enzymatic technique to create highly porous scaffold with a large surface to volume ratio, which makes them attractive as potential tissue scaffold in biomedical field. Scanning electron microscopy revealed that the surface of salt-leached films was more porous compared with the solvent-cast films, and hence, increased the degradation rate of salt-leached films. Apparently, enzymatic degradation behaviors of PHA films were determined by several factors such as monomer composition, crystallinity, molecular weight, porosity, and roughness of the surface. The hydrophilicity and water uptake of degraded salt-leached film of P(3HB-co-70%4HB) were enhanced by incorporating chitosan or alginate. Salt-leached technique followed by partial enzymatic degradation would enhance the cell attachment and suitable for biomedical as a scaffold. Springer Verlag 2013-04-19 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62129/1/faezah%20ansari%202013-degradation.pdf application/pdf en http://irep.iium.edu.my/62129/7/62129_Preparation%20and%20characterization_SCOPUS.pdf Ansari, Nor Faezah and Abdullah, Amirul Al-Ashraf (2013) Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications. Applied Biochemistry and Biotechnology, 170 (3). pp. 690-709. ISSN 0273-2289 https://link.springer.com/article/10.1007/s12010-013-0216-0 10.1007/s12010-013-0216-0
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Ansari, Nor Faezah Abdullah, Amirul Al-Ashraf Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications
description	Polyhydroxyalkanoates (PHAs) are hydrophobic biodegradable thermoplastics that have received considerable attention in biomedical applications due to their biocompatibility, mechanical properties, and biodegradability. In this study, the degradation rate was regulated by optimizing the interaction of parameters that influence the enzymatic degradation of P(3HB) film using response surface methodology (RSM). The RSM model was experimentally validated yielding a maximum 21 % weight loss, which represents onefold increment in percentage weight loss in comparison with the conventional method. By using the optimized condition, the enzymatic degradation by an extracellular PHA depolymerase from Acidovorax sp. DP5 was studied at 37 °C and pH 9.0 on different types of PHA films with various monomer compositions. Surface modification of scaffold was employed using enzymatic technique to create highly porous scaffold with a large surface to volume ratio, which makes them attractive as potential tissue scaffold in biomedical field. Scanning electron microscopy revealed that the surface of salt-leached films was more porous compared with the solvent-cast films, and hence, increased the degradation rate of salt-leached films. Apparently, enzymatic degradation behaviors of PHA films were determined by several factors such as monomer composition, crystallinity, molecular weight, porosity, and roughness of the surface. The hydrophilicity and water uptake of degraded salt-leached film of P(3HB-co-70%4HB) were enhanced by incorporating chitosan or alginate. Salt-leached technique followed by partial enzymatic degradation would enhance the cell attachment and suitable for biomedical as a scaffold.
format	Article
author	Ansari, Nor Faezah Abdullah, Amirul Al-Ashraf
author_facet	Ansari, Nor Faezah Abdullah, Amirul Al-Ashraf
author_sort	Ansari, Nor Faezah
title	Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications
title_short	Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications
title_full	Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications
title_fullStr	Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications
title_full_unstemmed	Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications
title_sort	preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications
publisher	Springer Verlag
publishDate	2013
url	http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/ http://irep.iium.edu.my/62129/1/faezah%20ansari%202013-degradation.pdf http://irep.iium.edu.my/62129/7/62129_Preparation%20and%20characterization_SCOPUS.pdf
first_indexed	2023-09-18T21:28:05Z
last_indexed	2023-09-18T21:28:05Z
_version_	1777412326429818880

Preparation and characterization of polyhydroxyalkanoates macroporous scaffold through enzyme-mediated modifications

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