Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum
Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees. APG is rich in soluble dietary fibers as well as organic compounds. In this study quantitation of the levels of total phenolics content (TPC) and antioxidant activities were conduc...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
2017
|
Subjects: | |
Online Access: | http://irep.iium.edu.my/62294/ http://irep.iium.edu.my/62294/ http://irep.iium.edu.my/62294/1/62294_Effect%20of%20solvent%20types%20on%20phenolics.pdf http://irep.iium.edu.my/62294/7/62294_Effect%20of%20solvent%20types%20on%20phenolics%20content_scopus.pdf |
id |
iium-62294 |
---|---|
recordtype |
eprints |
spelling |
iium-622942018-04-19T09:04:57Z http://irep.iium.edu.my/62294/ Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum MohammedElnour, A. A. Hassan Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md Zahangir Musa, Khalid Hamid Abdullah, Aminah Q Science (General) SD Forestry T Technology (General) Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees. APG is rich in soluble dietary fibers as well as organic compounds. In this study quantitation of the levels of total phenolics content (TPC) and antioxidant activities were conducted using ABTS and CUPRAC assays for APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2’-azino-bis-(3-mthylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation deculturization and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. The solvent Methanol (50%) gave the best extraction ratio for APG presented by highest (TPC 60.78 mg GAE/100g of DW, CUPRAC 34.65 mg TE/100g DW, and, ABTS about 37.65 mg TE/100g DW respectively), followed by ethanol 50% extract. On the other hand, pure methanol showed the lowest TPC 5.33 mg GAE/100g of DW, ABTS 10.9 mg TE/100g DW, and CUPRAC 7.80 mg TE/100g DW, values respectively. Therefore, the variation in the antioxidant capacity of extracts was possibly due to the difference of polarity, immiscibility and the nature of the APG compounds extracted using various solvents. The higher content of antioxidant activity in APG shall be useful to human health if it is properly utilized. Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62294/1/62294_Effect%20of%20solvent%20types%20on%20phenolics.pdf application/pdf en http://irep.iium.edu.my/62294/7/62294_Effect%20of%20solvent%20types%20on%20phenolics%20content_scopus.pdf MohammedElnour, A. A. Hassan and Mirghani, Mohamed Elwathig Saeed and Kabbashi, Nassereldeen Ahmed and Alam, Md Zahangir and Musa, Khalid Hamid and Abdullah, Aminah (2017) Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum. International Food Research Journal (IFRJ), 24 (Suppl.). pp. 369-377. ISSN 1985-4668 E-ISSN 2231-7546 http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(17)%20R1.pdf |
repository_type |
Digital Repository |
institution_category |
Local University |
institution |
International Islamic University Malaysia |
building |
IIUM Repository |
collection |
Online Access |
language |
English English |
topic |
Q Science (General) SD Forestry T Technology (General) |
spellingShingle |
Q Science (General) SD Forestry T Technology (General) MohammedElnour, A. A. Hassan Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md Zahangir Musa, Khalid Hamid Abdullah, Aminah Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum |
description |
Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees. APG is rich in soluble dietary fibers as well as organic compounds. In this study quantitation of the levels of total phenolics content (TPC) and antioxidant activities were conducted using ABTS and CUPRAC assays for APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2’-azino-bis-(3-mthylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation deculturization and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. The solvent Methanol (50%) gave the best extraction ratio for APG presented by highest (TPC 60.78 mg GAE/100g of DW, CUPRAC 34.65 mg TE/100g DW, and, ABTS about 37.65 mg TE/100g DW respectively), followed by ethanol 50% extract. On the other hand, pure methanol showed the lowest TPC 5.33 mg GAE/100g of DW, ABTS 10.9 mg TE/100g DW, and CUPRAC 7.80 mg TE/100g DW, values respectively. Therefore, the variation in the antioxidant capacity of extracts was possibly due to the difference of polarity, immiscibility and the nature of the APG compounds extracted using various solvents. The higher content of antioxidant activity in APG shall be useful to human health if it is properly
utilized. |
format |
Article |
author |
MohammedElnour, A. A. Hassan Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md Zahangir Musa, Khalid Hamid Abdullah, Aminah |
author_facet |
MohammedElnour, A. A. Hassan Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md Zahangir Musa, Khalid Hamid Abdullah, Aminah |
author_sort |
MohammedElnour, A. A. Hassan |
title |
Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum |
title_short |
Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum |
title_full |
Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum |
title_fullStr |
Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum |
title_full_unstemmed |
Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum |
title_sort |
effect of solvent types on phenolics content and antioxidant activities of acacia polyacantha gum |
publisher |
Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) |
publishDate |
2017 |
url |
http://irep.iium.edu.my/62294/ http://irep.iium.edu.my/62294/ http://irep.iium.edu.my/62294/1/62294_Effect%20of%20solvent%20types%20on%20phenolics.pdf http://irep.iium.edu.my/62294/7/62294_Effect%20of%20solvent%20types%20on%20phenolics%20content_scopus.pdf |
first_indexed |
2023-09-18T21:28:19Z |
last_indexed |
2023-09-18T21:28:19Z |
_version_ |
1777412340934770688 |