Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum

Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees. APG is rich in soluble dietary fibers as well as organic compounds. In this study quantitation of the levels of total phenolics content (TPC) and antioxidant activities were conduc...

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Main Authors: MohammedElnour, A. A. Hassan, Mirghani, Mohamed Elwathig Saeed, Kabbashi, Nassereldeen Ahmed, Alam, Md Zahangir, Musa, Khalid Hamid, Abdullah, Aminah
Format: Article
Language:English
English
Published: Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017
Subjects:
Online Access:http://irep.iium.edu.my/62294/
http://irep.iium.edu.my/62294/
http://irep.iium.edu.my/62294/1/62294_Effect%20of%20solvent%20types%20on%20phenolics.pdf
http://irep.iium.edu.my/62294/7/62294_Effect%20of%20solvent%20types%20on%20phenolics%20content_scopus.pdf
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spelling iium-622942018-04-19T09:04:57Z http://irep.iium.edu.my/62294/ Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum MohammedElnour, A. A. Hassan Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md Zahangir Musa, Khalid Hamid Abdullah, Aminah Q Science (General) SD Forestry T Technology (General) Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees. APG is rich in soluble dietary fibers as well as organic compounds. In this study quantitation of the levels of total phenolics content (TPC) and antioxidant activities were conducted using ABTS and CUPRAC assays for APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2’-azino-bis-(3-mthylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation deculturization and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. The solvent Methanol (50%) gave the best extraction ratio for APG presented by highest (TPC 60.78 mg GAE/100g of DW, CUPRAC 34.65 mg TE/100g DW, and, ABTS about 37.65 mg TE/100g DW respectively), followed by ethanol 50% extract. On the other hand, pure methanol showed the lowest TPC 5.33 mg GAE/100g of DW, ABTS 10.9 mg TE/100g DW, and CUPRAC 7.80 mg TE/100g DW, values respectively. Therefore, the variation in the antioxidant capacity of extracts was possibly due to the difference of polarity, immiscibility and the nature of the APG compounds extracted using various solvents. The higher content of antioxidant activity in APG shall be useful to human health if it is properly utilized. Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62294/1/62294_Effect%20of%20solvent%20types%20on%20phenolics.pdf application/pdf en http://irep.iium.edu.my/62294/7/62294_Effect%20of%20solvent%20types%20on%20phenolics%20content_scopus.pdf MohammedElnour, A. A. Hassan and Mirghani, Mohamed Elwathig Saeed and Kabbashi, Nassereldeen Ahmed and Alam, Md Zahangir and Musa, Khalid Hamid and Abdullah, Aminah (2017) Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum. International Food Research Journal (IFRJ), 24 (Suppl.). pp. 369-377. ISSN 1985-4668 E-ISSN 2231-7546 http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(17)%20R1.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic Q Science (General)
SD Forestry
T Technology (General)
spellingShingle Q Science (General)
SD Forestry
T Technology (General)
MohammedElnour, A. A. Hassan
Mirghani, Mohamed Elwathig Saeed
Kabbashi, Nassereldeen Ahmed
Alam, Md Zahangir
Musa, Khalid Hamid
Abdullah, Aminah
Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum
description Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees. APG is rich in soluble dietary fibers as well as organic compounds. In this study quantitation of the levels of total phenolics content (TPC) and antioxidant activities were conducted using ABTS and CUPRAC assays for APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2’-azino-bis-(3-mthylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation deculturization and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. The solvent Methanol (50%) gave the best extraction ratio for APG presented by highest (TPC 60.78 mg GAE/100g of DW, CUPRAC 34.65 mg TE/100g DW, and, ABTS about 37.65 mg TE/100g DW respectively), followed by ethanol 50% extract. On the other hand, pure methanol showed the lowest TPC 5.33 mg GAE/100g of DW, ABTS 10.9 mg TE/100g DW, and CUPRAC 7.80 mg TE/100g DW, values respectively. Therefore, the variation in the antioxidant capacity of extracts was possibly due to the difference of polarity, immiscibility and the nature of the APG compounds extracted using various solvents. The higher content of antioxidant activity in APG shall be useful to human health if it is properly utilized.
format Article
author MohammedElnour, A. A. Hassan
Mirghani, Mohamed Elwathig Saeed
Kabbashi, Nassereldeen Ahmed
Alam, Md Zahangir
Musa, Khalid Hamid
Abdullah, Aminah
author_facet MohammedElnour, A. A. Hassan
Mirghani, Mohamed Elwathig Saeed
Kabbashi, Nassereldeen Ahmed
Alam, Md Zahangir
Musa, Khalid Hamid
Abdullah, Aminah
author_sort MohammedElnour, A. A. Hassan
title Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum
title_short Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum
title_full Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum
title_fullStr Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum
title_full_unstemmed Effect of solvent types on phenolics content and antioxidant activities of Acacia polyacantha gum
title_sort effect of solvent types on phenolics content and antioxidant activities of acacia polyacantha gum
publisher Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
publishDate 2017
url http://irep.iium.edu.my/62294/
http://irep.iium.edu.my/62294/
http://irep.iium.edu.my/62294/1/62294_Effect%20of%20solvent%20types%20on%20phenolics.pdf
http://irep.iium.edu.my/62294/7/62294_Effect%20of%20solvent%20types%20on%20phenolics%20content_scopus.pdf
first_indexed 2023-09-18T21:28:19Z
last_indexed 2023-09-18T21:28:19Z
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