Spermatogenesis in vitro: the future promise for azoospermia patients

Male inferti lity becomes a worldwide problem and contributed to 50% of infertility cases. The main cause of male inferti lity is spermatogenes is failure such as oligozoospermia and azoospermia. Azoospermia is defined as absence of spermatozoa from semen and it is related to incomplete spermatog...

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Main Authors:	Abdul Wahab, Azantee Yazmie, Ramli, Roszaman, Md Isa, Muhammad Lokman
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	RG Gynecology and obstetrics
Online Access:	http://irep.iium.edu.my/62575/ http://irep.iium.edu.my/62575/1/62575_Spermatogenesis%20in%20vitro.pdf

id	iium-62575
recordtype	eprints
spelling	iium-625752018-04-02T03:36:25Z http://irep.iium.edu.my/62575/ Spermatogenesis in vitro: the future promise for azoospermia patients Abdul Wahab, Azantee Yazmie Ramli, Roszaman Md Isa, Muhammad Lokman RG Gynecology and obstetrics Male inferti lity becomes a worldwide problem and contributed to 50% of infertility cases. The main cause of male inferti lity is spermatogenes is failure such as oligozoospermia and azoospermia. Azoospermia is defined as absence of spermatozoa from semen and it is related to incomplete spermatogenesis process which related to genetic disorder, hormonal problem or testicular failure. Recreating human spermatogenesis outside of its original environment is a scientific curiosity in andrology world and a quest for male fertility treatment. It has a huge impact on our understanding on ph) siology and pathway of genetic in male reproduction using a well-characterized model of human spermatogenesis. Animal studies have provided us the knowledge of gonadogenesis, spermatogenesis and steroidogenesis based on the histology, immunohistochemistry, hormonal assays and phenotype of gene alterations. In vitro spermatogenesis in azoospermic patients has long been attempted, however, it remains challenging due to limitation of cu lture system. Human embryonic stem cell (hESC) media were widely used for embryonic stem cell culture and some of these studies had derived the male germ cells from embryonic stem cells. This media could possible initiate the SSCs-like cell from adult testicu lar stem cells. The purpose of this study is to proliferate the SSCs-like cell and differentiate into next stage of spermatogenic cell using modified hESC media. Gene expressions were done to determine the spermatogenesis stages ach ieved. 2017-12-16 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/62575/1/62575_Spermatogenesis%20in%20vitro.pdf Abdul Wahab, Azantee Yazmie and Ramli, Roszaman and Md Isa, Muhammad Lokman (2017) Spermatogenesis in vitro: the future promise for azoospermia patients. In: The Annual Conference of The Malaysian Clinical Embryologist (MACE 2017), 16th-17th Dec. 2017, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RG Gynecology and obstetrics
spellingShingle	RG Gynecology and obstetrics Abdul Wahab, Azantee Yazmie Ramli, Roszaman Md Isa, Muhammad Lokman Spermatogenesis in vitro: the future promise for azoospermia patients
description	Male inferti lity becomes a worldwide problem and contributed to 50% of infertility cases. The main cause of male inferti lity is spermatogenes is failure such as oligozoospermia and azoospermia. Azoospermia is defined as absence of spermatozoa from semen and it is related to incomplete spermatogenesis process which related to genetic disorder, hormonal problem or testicular failure. Recreating human spermatogenesis outside of its original environment is a scientific curiosity in andrology world and a quest for male fertility treatment. It has a huge impact on our understanding on ph) siology and pathway of genetic in male reproduction using a well-characterized model of human spermatogenesis. Animal studies have provided us the knowledge of gonadogenesis, spermatogenesis and steroidogenesis based on the histology, immunohistochemistry, hormonal assays and phenotype of gene alterations. In vitro spermatogenesis in azoospermic patients has long been attempted, however, it remains challenging due to limitation of cu lture system. Human embryonic stem cell (hESC) media were widely used for embryonic stem cell culture and some of these studies had derived the male germ cells from embryonic stem cells. This media could possible initiate the SSCs-like cell from adult testicu lar stem cells. The purpose of this study is to proliferate the SSCs-like cell and differentiate into next stage of spermatogenic cell using modified hESC media. Gene expressions were done to determine the spermatogenesis stages ach ieved.
format	Conference or Workshop Item
author	Abdul Wahab, Azantee Yazmie Ramli, Roszaman Md Isa, Muhammad Lokman
author_facet	Abdul Wahab, Azantee Yazmie Ramli, Roszaman Md Isa, Muhammad Lokman
author_sort	Abdul Wahab, Azantee Yazmie
title	Spermatogenesis in vitro: the future promise for azoospermia patients
title_short	Spermatogenesis in vitro: the future promise for azoospermia patients
title_full	Spermatogenesis in vitro: the future promise for azoospermia patients
title_fullStr	Spermatogenesis in vitro: the future promise for azoospermia patients
title_full_unstemmed	Spermatogenesis in vitro: the future promise for azoospermia patients
title_sort	spermatogenesis in vitro: the future promise for azoospermia patients
publishDate	2017
url	http://irep.iium.edu.my/62575/ http://irep.iium.edu.my/62575/1/62575_Spermatogenesis%20in%20vitro.pdf
first_indexed	2023-09-18T21:28:40Z
last_indexed	2023-09-18T21:28:40Z
_version_	1777412363771707392

Spermatogenesis in vitro: the future promise for azoospermia patients

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