Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model

This study aimed to examine the effects of tualang honey against the high cholesterol diet induced biochemical and histological changes in the kidney, liver and pancreas. Methods: Female Sprague-Dawley rats were used where the control group (n = 5) was fed with commercial rat pellet; the high choles...

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Main Authors:	Abdul Rahim, Roslina, Muhammad, Naznin, Abdullah, Nor Zamzila
Format:	Monograph
Language:	English
Published:	2018
Subjects:	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RM Therapeutics. Pharmacology RM214 Diet therapy. Diet and dietectics in disease
Online Access:	http://irep.iium.edu.my/62762/ http://irep.iium.edu.my/62762/3/62762_MOLECULAR%20MECHANISM%20OF%20TUALANG%20HONEY.pdf

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recordtype	eprints
spelling	iium-627622018-03-20T02:59:31Z http://irep.iium.edu.my/62762/ Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model Abdul Rahim, Roslina Muhammad, Naznin Abdullah, Nor Zamzila QP Physiology R Medicine (General) RB Pathology RC Internal medicine RM Therapeutics. Pharmacology RM214 Diet therapy. Diet and dietectics in disease This study aimed to examine the effects of tualang honey against the high cholesterol diet induced biochemical and histological changes in the kidney, liver and pancreas. Methods: Female Sprague-Dawley rats were used where the control group (n = 5) was fed with commercial rat pellet; the high cholesterol diet (HCD) group (n= 5) was given 12% cholesterol diet; while the HCD with tualang honey (HCD+TH) group (n =5) was fed with 12% cholesterol diet with daily 1.4 g/kg/day of tualang honey. Biochemical analyses for lipid profile and renal function test were performed at completed 48 hours, 7 days, and 6 weeks. The rats were sacrificed at completed 6 weeks and the kidneys were harvested and subjected to histopathological examination. Blood biochemical analysis were also analysed at 1 week and 6 weeks for liver function test, fasting insulin, fasting glucose and HOMA-IR. The liver and pancreas tissues were harvested at the end of 6 weeks for histological examination. Results: The cholesterol diet induction resulted in dyslipidaemia and abnormal liver function. The HCD+TH group have shown an increase in total cholesterol, LDL-c, ALP levels and decreased TG, HDL-c and AST levels significantly at the end of 6 weeks compared to HCD group. Consumption of 12% cholesterol diet for six weeks resulted in an increment of the mean serum creatinine level of the HCD and HCD+TH groups to 1.5 times the control level at the completed 7 days. Also overall both the mean serum creatinine and blood urea levels were higher in HCD group than the control group. With tualang honey supplementation, the mean serum creatinine level showed significant reduction at 48 hours in the HCD+TH group as compared to the HCD group. There was also a reduction in the mean serum creatinine level at the completed 6 weeks. . Histopathologically the kidneys exhibited segmental mesangial hypercellularity and mesangial matrix expansion of almost all the glomeruli in both HCD and HCD+TH groups. The mean plasma glucose level was elevated at 1 week in the HCD group. Plasma insulin levels were higher in HCD+TH group at both 1 and 6 weeks. The HOMA-IR was also higher at 1 week in the HCD+TH group. The liver histology of both HCD and HCD+TH groups showed steatohepatitis with minimal hepatocyte degeneration while the pancreatic sections revealed no abnormalities. Conclusion: The 12% cholesterol diet of 6 weeks duration in this study did induce some features of NASH with dyslipidaemia with abnormal liver profile and also acute kidney injury. The tualang honey exerts some degree of renoprotective effect against high cholesterol diet induced kidney injury, but exhibited no effect on dyslipidaemia and histopathological changes in liver and pancreas. 2018-03-17 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/62762/3/62762_MOLECULAR%20MECHANISM%20OF%20TUALANG%20HONEY.pdf Abdul Rahim, Roslina and Muhammad, Naznin and Abdullah, Nor Zamzila (2018) Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model. Research Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RM Therapeutics. Pharmacology RM214 Diet therapy. Diet and dietectics in disease
spellingShingle	QP Physiology R Medicine (General) RB Pathology RC Internal medicine RM Therapeutics. Pharmacology RM214 Diet therapy. Diet and dietectics in disease Abdul Rahim, Roslina Muhammad, Naznin Abdullah, Nor Zamzila Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model
description	This study aimed to examine the effects of tualang honey against the high cholesterol diet induced biochemical and histological changes in the kidney, liver and pancreas. Methods: Female Sprague-Dawley rats were used where the control group (n = 5) was fed with commercial rat pellet; the high cholesterol diet (HCD) group (n= 5) was given 12% cholesterol diet; while the HCD with tualang honey (HCD+TH) group (n =5) was fed with 12% cholesterol diet with daily 1.4 g/kg/day of tualang honey. Biochemical analyses for lipid profile and renal function test were performed at completed 48 hours, 7 days, and 6 weeks. The rats were sacrificed at completed 6 weeks and the kidneys were harvested and subjected to histopathological examination. Blood biochemical analysis were also analysed at 1 week and 6 weeks for liver function test, fasting insulin, fasting glucose and HOMA-IR. The liver and pancreas tissues were harvested at the end of 6 weeks for histological examination. Results: The cholesterol diet induction resulted in dyslipidaemia and abnormal liver function. The HCD+TH group have shown an increase in total cholesterol, LDL-c, ALP levels and decreased TG, HDL-c and AST levels significantly at the end of 6 weeks compared to HCD group. Consumption of 12% cholesterol diet for six weeks resulted in an increment of the mean serum creatinine level of the HCD and HCD+TH groups to 1.5 times the control level at the completed 7 days. Also overall both the mean serum creatinine and blood urea levels were higher in HCD group than the control group. With tualang honey supplementation, the mean serum creatinine level showed significant reduction at 48 hours in the HCD+TH group as compared to the HCD group. There was also a reduction in the mean serum creatinine level at the completed 6 weeks. . Histopathologically the kidneys exhibited segmental mesangial hypercellularity and mesangial matrix expansion of almost all the glomeruli in both HCD and HCD+TH groups. The mean plasma glucose level was elevated at 1 week in the HCD group. Plasma insulin levels were higher in HCD+TH group at both 1 and 6 weeks. The HOMA-IR was also higher at 1 week in the HCD+TH group. The liver histology of both HCD and HCD+TH groups showed steatohepatitis with minimal hepatocyte degeneration while the pancreatic sections revealed no abnormalities. Conclusion: The 12% cholesterol diet of 6 weeks duration in this study did induce some features of NASH with dyslipidaemia with abnormal liver profile and also acute kidney injury. The tualang honey exerts some degree of renoprotective effect against high cholesterol diet induced kidney injury, but exhibited no effect on dyslipidaemia and histopathological changes in liver and pancreas.
format	Monograph
author	Abdul Rahim, Roslina Muhammad, Naznin Abdullah, Nor Zamzila
author_facet	Abdul Rahim, Roslina Muhammad, Naznin Abdullah, Nor Zamzila
author_sort	Abdul Rahim, Roslina
title	Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model
title_short	Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model
title_full	Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model
title_fullStr	Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model
title_full_unstemmed	Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model
title_sort	molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (nash) animal model
publishDate	2018
url	http://irep.iium.edu.my/62762/ http://irep.iium.edu.my/62762/3/62762_MOLECULAR%20MECHANISM%20OF%20TUALANG%20HONEY.pdf
first_indexed	2023-09-18T21:28:55Z
last_indexed	2023-09-18T21:28:55Z
_version_	1777412378749566976

Molecular mechanism of tualang honey on 12% cholesterol diet induced nonalcoholic steatohepatitis (NASH) animal model

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