Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors

Monitoring the effectiveness of drugs on cancer cells is crucial for chemotherapeutics studies. Invitro cell-based biosensors can be used as an alternative for characteristic studies of cells’ response to drugs. Cell-based sensors provide real-time measurements and require smaller sample volumes...

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Main Authors:	Ibrahim, Irmanisha, Nordin, Anis Nurashikin, Mohd Mansor, Ahmad Fairuzabadi, Hashim, Yumi Zuhanis Has-Yun, Voiculescu, Loana
Format:	Article
Language:	English English
Published:	Institute of Advanced Engineering and Science 2017
Subjects:	QR Microbiology TA Engineering (General). Civil engineering (General)
Online Access:	http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/1/62788_Content%20Cytotoxicity%20Studies%20of%20Colorectal%20_article.pdf http://irep.iium.edu.my/62788/2/62788_Content%20Cytotoxicity%20Studies%20of%20Colorectal%20_scopus.pdf

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spelling	iium-627882018-04-09T03:15:15Z http://irep.iium.edu.my/62788/ Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors Ibrahim, Irmanisha Nordin, Anis Nurashikin Mohd Mansor, Ahmad Fairuzabadi Hashim, Yumi Zuhanis Has-Yun Voiculescu, Loana QR Microbiology TA Engineering (General). Civil engineering (General) Monitoring the effectiveness of drugs on cancer cells is crucial for chemotherapeutics studies. Invitro cell-based biosensors can be used as an alternative for characteristic studies of cells’ response to drugs. Cell-based sensors provide real-time measurements and require smaller sample volumes compared to conventional T-flask measurement methods. This paper presents a biosensor that detects in real-time, impedance variations of human colon cancer, HCT-116 cells when treated with anti-cancer agent, 5-Fluorouracil (5-FU). Two different extracellular matrix (ECM); polyaniline and gelatin were tested and evaluated in terms of attachment quality. Polyaniline was found to provide the best attachment for HCT-116 cells and was used for cytotoxicity studies. Cytokinetic behavior indicated that 5-FU inhibited HCT-116 cells at IC50 of 6.8 µg/mL. Trypan blue exclusion method for testing cell viability was used to validate the impedance measurements, where the cancer cell concentrations were reduced to ~35% when treated with 2.5 µg/mL, and 50% when treated with 6.8 µg/mL. The results generated by the microfabricated impedance biosensor are comparable to the Trypan blue method since both gave similar cell growth trend. It can be concluded that the impedance biosensor has potential to be used as an alternative method in drug testing applications Institute of Advanced Engineering and Science 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62788/1/62788_Content%20Cytotoxicity%20Studies%20of%20Colorectal%20_article.pdf application/pdf en http://irep.iium.edu.my/62788/2/62788_Content%20Cytotoxicity%20Studies%20of%20Colorectal%20_scopus.pdf Ibrahim, Irmanisha and Nordin, Anis Nurashikin and Mohd Mansor, Ahmad Fairuzabadi and Hashim, Yumi Zuhanis Has-Yun and Voiculescu, Loana (2017) Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors. Bulletin of Electrical Engineering and Informatics, 6 (4). pp. 317-326. ISSN 2089-3191 http://journal.portalgaruda.org/index.php/EEI/article/view/851/497 10.11591/eei.v6i4.851
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology TA Engineering (General). Civil engineering (General)
spellingShingle	QR Microbiology TA Engineering (General). Civil engineering (General) Ibrahim, Irmanisha Nordin, Anis Nurashikin Mohd Mansor, Ahmad Fairuzabadi Hashim, Yumi Zuhanis Has-Yun Voiculescu, Loana Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors
description	Monitoring the effectiveness of drugs on cancer cells is crucial for chemotherapeutics studies. Invitro cell-based biosensors can be used as an alternative for characteristic studies of cells’ response to drugs. Cell-based sensors provide real-time measurements and require smaller sample volumes compared to conventional T-flask measurement methods. This paper presents a biosensor that detects in real-time, impedance variations of human colon cancer, HCT-116 cells when treated with anti-cancer agent, 5-Fluorouracil (5-FU). Two different extracellular matrix (ECM); polyaniline and gelatin were tested and evaluated in terms of attachment quality. Polyaniline was found to provide the best attachment for HCT-116 cells and was used for cytotoxicity studies. Cytokinetic behavior indicated that 5-FU inhibited HCT-116 cells at IC50 of 6.8 µg/mL. Trypan blue exclusion method for testing cell viability was used to validate the impedance measurements, where the cancer cell concentrations were reduced to ~35% when treated with 2.5 µg/mL, and 50% when treated with 6.8 µg/mL. The results generated by the microfabricated impedance biosensor are comparable to the Trypan blue method since both gave similar cell growth trend. It can be concluded that the impedance biosensor has potential to be used as an alternative method in drug testing applications
format	Article
author	Ibrahim, Irmanisha Nordin, Anis Nurashikin Mohd Mansor, Ahmad Fairuzabadi Hashim, Yumi Zuhanis Has-Yun Voiculescu, Loana
author_facet	Ibrahim, Irmanisha Nordin, Anis Nurashikin Mohd Mansor, Ahmad Fairuzabadi Hashim, Yumi Zuhanis Has-Yun Voiculescu, Loana
author_sort	Ibrahim, Irmanisha
title	Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors
title_short	Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors
title_full	Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors
title_fullStr	Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors
title_full_unstemmed	Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors
title_sort	content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors
publisher	Institute of Advanced Engineering and Science
publishDate	2017
url	http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/ http://irep.iium.edu.my/62788/1/62788_Content%20Cytotoxicity%20Studies%20of%20Colorectal%20_article.pdf http://irep.iium.edu.my/62788/2/62788_Content%20Cytotoxicity%20Studies%20of%20Colorectal%20_scopus.pdf
first_indexed	2023-09-18T21:28:58Z
last_indexed	2023-09-18T21:28:58Z
_version_	1777412381687676928

Content cytotoxicity studies of colorectal carcinoma cells using printed impedance sensors

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