Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres

Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycol...

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Main Authors:	Ansary, Rezaul H., Rahman, Mohd. Mokhlesur, Mohamad, Nasir A.N., Arrif, Tengku M, Abdul Latif, Ahmad Zubaidi, Katas, Haliza, Wan Nik, Wan Sani, Awang, Mohamed
Format:	Article
Language:	English English
Published:	MDPI AG 2017
Subjects:	QD Chemistry QR Microbiology RE Ophthalmology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/1/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_article.pdf http://irep.iium.edu.my/62789/2/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_scopus.pdf

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recordtype	eprints
spelling	iium-627892018-06-21T02:34:22Z http://irep.iium.edu.my/62789/ Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres Ansary, Rezaul H. Rahman, Mohd. Mokhlesur Mohamad, Nasir A.N. Arrif, Tengku M Abdul Latif, Ahmad Zubaidi Katas, Haliza Wan Nik, Wan Sani Awang, Mohamed QD Chemistry QR Microbiology RE Ophthalmology RS Pharmacy and materia medica Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52 ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme. MDPI AG 2017-10-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62789/1/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_article.pdf application/pdf en http://irep.iium.edu.my/62789/2/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_scopus.pdf Ansary, Rezaul H. and Rahman, Mohd. Mokhlesur and Mohamad, Nasir A.N. and Arrif, Tengku M and Abdul Latif, Ahmad Zubaidi and Katas, Haliza and Wan Nik, Wan Sani and Awang, Mohamed (2017) Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres. Polymers, 9 (10). pp. 1-17. ISSN 2073-4360 http://www.mdpi.com/2073-4360/9/10/485/htm 10.3390/polym9100485
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry QR Microbiology RE Ophthalmology RS Pharmacy and materia medica
spellingShingle	QD Chemistry QR Microbiology RE Ophthalmology RS Pharmacy and materia medica Ansary, Rezaul H. Rahman, Mohd. Mokhlesur Mohamad, Nasir A.N. Arrif, Tengku M Abdul Latif, Ahmad Zubaidi Katas, Haliza Wan Nik, Wan Sani Awang, Mohamed Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres
description	Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52 ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme.
format	Article
author	Ansary, Rezaul H. Rahman, Mohd. Mokhlesur Mohamad, Nasir A.N. Arrif, Tengku M Abdul Latif, Ahmad Zubaidi Katas, Haliza Wan Nik, Wan Sani Awang, Mohamed
author_facet	Ansary, Rezaul H. Rahman, Mohd. Mokhlesur Mohamad, Nasir A.N. Arrif, Tengku M Abdul Latif, Ahmad Zubaidi Katas, Haliza Wan Nik, Wan Sani Awang, Mohamed
author_sort	Ansary, Rezaul H.
title	Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres
title_short	Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres
title_full	Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres
title_fullStr	Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres
title_full_unstemmed	Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres
title_sort	controlled release of lysozyme from double-walled poly(lactide-co-glycolide) (plga) microspheres
publisher	MDPI AG
publishDate	2017
url	http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/1/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_article.pdf http://irep.iium.edu.my/62789/2/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_scopus.pdf
first_indexed	2023-09-18T21:28:58Z
last_indexed	2023-09-18T21:28:58Z
_version_	1777412381831331840

Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres

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