Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)

Currently, virus separation process is mainly conducted by using ultracentrifugation or sucrose gradient electrophoresis methods. Both methods however have several drawbacks wherein the ultracentrifugation method requires very high rotational speed to separate the virus while the sucrose gradient el...

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Main Authors:	Mel, Maizirwan, Arifin, Mohd Azmir, M Pauzi, Sazana
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/6299/ http://irep.iium.edu.my/6299/1/Recovery_Virus_by_TFF.pdf

id	iium-6299
recordtype	eprints
spelling	iium-62992011-11-21T19:54:06Z http://irep.iium.edu.my/6299/ Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF) Mel, Maizirwan Arifin, Mohd Azmir M Pauzi, Sazana TP155 Chemical engineering TP248.13 Biotechnology Currently, virus separation process is mainly conducted by using ultracentrifugation or sucrose gradient electrophoresis methods. Both methods however have several drawbacks wherein the ultracentrifugation method requires very high rotational speed to separate the virus while the sucrose gradient electrophoresis method is very time consuming. Alternative to both methods are by using Tangential Flow Filtration (TFF). In this study, we have separated and concentrated the Newcastle Disease Virus (NDV) harvested from embryonic specific pathogen free (SPF) eggs using TFF method by utilizing microfilter membrane with pore size of 0.45μm. Hollow fiber membrane was selected as filter because of their chemical and thermal stability. The study was conducted according to the design developed using Taguchi method which consists of selected three parameters and two levels of factors. The results revealed that higher pressure input (Pin), lower pressure output (Pout), and higher virus concentration led to higher virus titer. Optimum Trans-Membrane-Pressure (TMP) value of 15 psi and virus concentration of 28% had given the maximum titer of the virus which was 512 haemagglutination assay (HA) unit. 2009-08-14 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/6299/1/Recovery_Virus_by_TFF.pdf Mel, Maizirwan and Arifin, Mohd Azmir and M Pauzi, Sazana (2009) Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF). In: 3rd International Conference on Chemical & Bioprocess Engineering , 12–14 August 2009, Kota Kinabalu, Sabah.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Mel, Maizirwan Arifin, Mohd Azmir M Pauzi, Sazana Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
description	Currently, virus separation process is mainly conducted by using ultracentrifugation or sucrose gradient electrophoresis methods. Both methods however have several drawbacks wherein the ultracentrifugation method requires very high rotational speed to separate the virus while the sucrose gradient electrophoresis method is very time consuming. Alternative to both methods are by using Tangential Flow Filtration (TFF). In this study, we have separated and concentrated the Newcastle Disease Virus (NDV) harvested from embryonic specific pathogen free (SPF) eggs using TFF method by utilizing microfilter membrane with pore size of 0.45μm. Hollow fiber membrane was selected as filter because of their chemical and thermal stability. The study was conducted according to the design developed using Taguchi method which consists of selected three parameters and two levels of factors. The results revealed that higher pressure input (Pin), lower pressure output (Pout), and higher virus concentration led to higher virus titer. Optimum Trans-Membrane-Pressure (TMP) value of 15 psi and virus concentration of 28% had given the maximum titer of the virus which was 512 haemagglutination assay (HA) unit.
format	Conference or Workshop Item
author	Mel, Maizirwan Arifin, Mohd Azmir M Pauzi, Sazana
author_facet	Mel, Maizirwan Arifin, Mohd Azmir M Pauzi, Sazana
author_sort	Mel, Maizirwan
title	Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_short	Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_full	Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_fullStr	Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_full_unstemmed	Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)
title_sort	recovery of virus producing ndv vaccine by tangential flow filtration (tff)
publishDate	2009
url	http://irep.iium.edu.my/6299/ http://irep.iium.edu.my/6299/1/Recovery_Virus_by_TFF.pdf
first_indexed	2023-09-18T20:15:11Z
last_indexed	2023-09-18T20:15:11Z
_version_	1777407740545597440

Recovery of virus producing NDV vaccine by Tangential Flow Filtration (TFF)

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