Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand
Background Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom is known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of krait venom that in...
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| Language: | English English English |
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Springer Nature
2018
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| Online Access: | http://irep.iium.edu.my/63012/ http://irep.iium.edu.my/63012/ http://irep.iium.edu.my/63012/ http://irep.iium.edu.my/63012/2/63012%20Non-neurotoxic%20activity%20of%20Malayan%20krait.pdf http://irep.iium.edu.my/63012/3/63012%20Non-neurotoxic%20activity%20of%20Malayan%20krait%20SCOPUS.pdf http://irep.iium.edu.my/63012/14/63012_Non-neurotoxic%20activity%20of%20Malayan%20krait%20_WOS.pdf |
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iium-63012 |
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eprints |
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iium-630122018-06-21T02:20:31Z http://irep.iium.edu.my/63012/ Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand Charoenpitakchai, Mongkon Wiwatwarayos, Kulachet Jaisupa, Nattapon Ahmad Rusmili, Muhamad Rusdi Mangmool, Supachoke Hodgson, Wayne C. Ruangpratheep, Chetana Chanhome, Lawan Chaisakul, Janeyuth RM Therapeutics. Pharmacology RM300 Drugs and their action Background Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom is known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of krait venom that include myotoxicity and nephrotoxicity. However, research on such non-neurotoxicity activities of Malayan krait venom is extremely limited. Thus, the aim of the present study was to determine the myotoxic, cytotoxic and nephrotoxic activities of B. candidus venoms from northeastern (BC-NE) and southern (BC-S) Thailand in experimentally envenomed rats. Methods Rats were administered Malayan krait (BC-NE or BC-S) venom (50 μg/kg, i.m.) or 0.9% NaCl solution (50 μL, i.m.) into the right hind limb. The animals were sacrificed 3, 6 and 24 h after venom administration. The right gastrocnemius muscle and both kidneys were collected for histopathological analysis. Blood samples were also taken for determination of creatine kinase (CK) and lactate dehydrogenase (LDH) levels. The human embryonic kidney cell line (HEK-293) was used in a cell proliferation assay to determine cytotoxic activity. Results Administration of BC-NE or BC-S venom (50 μg/kg, i.m.) caused time-dependent myotoxicity, characterized by an elevation of CK and LDH levels. Histopathological examination of skeletal muscle displayed marked muscle necrosis and myofiber disintegration 24 h following venom administration. Both Malayan krait venoms also induced extensive renal tubular injury with glomerular and interstitial congestion in rats. BC-NE and BC-S venoms (100–0.2 μg/mL) caused concentration-dependent cytotoxicity on the HEK-293 cell line. However, BC-NE venom (IC50 = 8 ± 1 μg/mL; at 24 h incubation; n = 4) was found to be significantly more cytotoxic than BC-S venom (IC50 = 15 ± 2 μg/mL; at 24 h incubation; n = 4). In addition, the PLA2 activity of BC-NE venom was significantly higher than that of BC-S venom. Conclusions This study found that Malayan krait venoms from both populations possess myotoxic, cytotoxic and nephrotoxic activities. These findings may aid in clinical diagnosis and treatment of envenomed patients in the future. Springer Nature 2018-03-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63012/2/63012%20Non-neurotoxic%20activity%20of%20Malayan%20krait.pdf application/pdf en http://irep.iium.edu.my/63012/3/63012%20Non-neurotoxic%20activity%20of%20Malayan%20krait%20SCOPUS.pdf application/pdf en http://irep.iium.edu.my/63012/14/63012_Non-neurotoxic%20activity%20of%20Malayan%20krait%20_WOS.pdf Charoenpitakchai, Mongkon and Wiwatwarayos, Kulachet and Jaisupa, Nattapon and Ahmad Rusmili, Muhamad Rusdi and Mangmool, Supachoke and Hodgson, Wayne C. and Ruangpratheep, Chetana and Chanhome, Lawan and Chaisakul, Janeyuth (2018) Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand. Journal of Venomous Animals and Toxins Including Tropical Diseases, 24 (1). pp. 1-9. ISSN 1678-9199 https://jvat.biomedcentral.com/track/pdf/10.1186/s40409-018-0146-y 10.1186/s40409-018-0146-y |
| repository_type |
Digital Repository |
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Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English English |
| topic |
RM Therapeutics. Pharmacology RM300 Drugs and their action |
| spellingShingle |
RM Therapeutics. Pharmacology RM300 Drugs and their action Charoenpitakchai, Mongkon Wiwatwarayos, Kulachet Jaisupa, Nattapon Ahmad Rusmili, Muhamad Rusdi Mangmool, Supachoke Hodgson, Wayne C. Ruangpratheep, Chetana Chanhome, Lawan Chaisakul, Janeyuth Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand |
| description |
Background
Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom is known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of krait venom that include myotoxicity and nephrotoxicity. However, research on such non-neurotoxicity activities of Malayan krait venom is extremely limited. Thus, the aim of the present study was to determine the myotoxic, cytotoxic and nephrotoxic activities of B. candidus venoms from northeastern (BC-NE) and southern (BC-S) Thailand in experimentally envenomed rats.
Methods
Rats were administered Malayan krait (BC-NE or BC-S) venom (50 μg/kg, i.m.) or 0.9% NaCl solution (50 μL, i.m.) into the right hind limb. The animals were sacrificed 3, 6 and 24 h after venom administration. The right gastrocnemius muscle and both kidneys were collected for histopathological analysis. Blood samples were also taken for determination of creatine kinase (CK) and lactate dehydrogenase (LDH) levels. The human embryonic kidney cell line (HEK-293) was used in a cell proliferation assay to determine cytotoxic activity.
Results
Administration of BC-NE or BC-S venom (50 μg/kg, i.m.) caused time-dependent myotoxicity, characterized by an elevation of CK and LDH levels. Histopathological examination of skeletal muscle displayed marked muscle necrosis and myofiber disintegration 24 h following venom administration. Both Malayan krait venoms also induced extensive renal tubular injury with glomerular and interstitial congestion in rats. BC-NE and BC-S venoms (100–0.2 μg/mL) caused concentration-dependent cytotoxicity on the HEK-293 cell line. However, BC-NE venom (IC50 = 8 ± 1 μg/mL; at 24 h incubation; n = 4) was found to be significantly more cytotoxic than BC-S venom (IC50 = 15 ± 2 μg/mL; at 24 h incubation; n = 4). In addition, the PLA2 activity of BC-NE venom was significantly higher than that of BC-S venom.
Conclusions
This study found that Malayan krait venoms from both populations possess myotoxic, cytotoxic and nephrotoxic activities. These findings may aid in clinical diagnosis and treatment of envenomed patients in the future. |
| format |
Article |
| author |
Charoenpitakchai, Mongkon Wiwatwarayos, Kulachet Jaisupa, Nattapon Ahmad Rusmili, Muhamad Rusdi Mangmool, Supachoke Hodgson, Wayne C. Ruangpratheep, Chetana Chanhome, Lawan Chaisakul, Janeyuth |
| author_facet |
Charoenpitakchai, Mongkon Wiwatwarayos, Kulachet Jaisupa, Nattapon Ahmad Rusmili, Muhamad Rusdi Mangmool, Supachoke Hodgson, Wayne C. Ruangpratheep, Chetana Chanhome, Lawan Chaisakul, Janeyuth |
| author_sort |
Charoenpitakchai, Mongkon |
| title |
Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand |
| title_short |
Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand |
| title_full |
Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand |
| title_fullStr |
Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand |
| title_full_unstemmed |
Non-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailand |
| title_sort |
non-neurotoxic activity of malayan krait (bungarus candidus) venom from thailand |
| publisher |
Springer Nature |
| publishDate |
2018 |
| url |
http://irep.iium.edu.my/63012/ http://irep.iium.edu.my/63012/ http://irep.iium.edu.my/63012/ http://irep.iium.edu.my/63012/2/63012%20Non-neurotoxic%20activity%20of%20Malayan%20krait.pdf http://irep.iium.edu.my/63012/3/63012%20Non-neurotoxic%20activity%20of%20Malayan%20krait%20SCOPUS.pdf http://irep.iium.edu.my/63012/14/63012_Non-neurotoxic%20activity%20of%20Malayan%20krait%20_WOS.pdf |
| first_indexed |
2023-09-18T21:29:19Z |
| last_indexed |
2023-09-18T21:29:19Z |
| _version_ |
1777412404670365696 |