Modeling and development of screen-printed impedance biosensor for cytotoxicity studies of lung carcinoma cells
Electrical cell-substrate impedance sensing (ECIS) is a powerful technique to monitor real-time cell behavior. In this study, an ECIS biosensor formed using two interdigitated electrode structures (IDEs) was used to monitor cell behavior and its response to toxicants. Three different sensors with...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English English English |
| Published: |
Springer
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/63149/ http://irep.iium.edu.my/63149/ http://irep.iium.edu.my/63149/ http://irep.iium.edu.my/63149/1/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance%20-Abstract-MyRA.pdf http://irep.iium.edu.my/63149/2/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance%20-SCOPUS.pdf http://irep.iium.edu.my/63149/13/63149_Modeling%20and%20development%20of%20screen-printed_WOS.pdf http://irep.iium.edu.my/63149/19/63149%20-Modeling%20and%20development%20of%20screen-printed%20impedance_article.pdf |
| Summary: | Electrical cell-substrate impedance sensing (ECIS) is a powerful technique to monitor real-time cell behavior. In this
study, an ECIS biosensor formed using two interdigitated electrode structures (IDEs) was used to monitor cell
behavior and its response to toxicants. Three different sensors with varied electrode spacing were first modeled using
COMSOL Multiphysics and then fabricated and tested. The silver/silver chloride IDEs were fabricated using a screenprinting
technique and incorporated with polydimethylsiloxane (PDMS) cell culture wells. To study the effectiveness of
the biosensor, A549 lung carcinoma cells were seeded in the culture wells together with collagen as an extracellular
matrix (ECM) to promote cell attachment on electrodes. A549 cells were cultured in the chambers and impedance
measurements were taken at 12-h intervals for 120 h. Cell index (CI) for both designs were calculated from the
impedance measurement and plotted in comparison with the growth profile of the cells in T-flasks. To verify that the
ECIS biosensor can also be used to study cell response to toxicants, the A549 cells were also treated with anti-cancer
drug, paclitaxel, and its responses were monitored over 5 days. Both simulation and experimental results show better
sensitivity for smaller spacing between electrodes. |
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