Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness
The optimum conditions for immobilization of β-Glucosidase on multiwalled carbon nanotubes (MWCNTs) and Ca-alginate beads were studied. The effectiveness of the reaction of the immobilized enzyme with pnitrophenyl-β-D-glucopyranoside as substrate is evaluated. For the same substrate concentration...
| Main Authors: | , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Kulliyyah of Engineering International Islamic University Malaysia P.O. Box 10, 50728 Kuala Lumpur, Malaysia
2016
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/63485/ http://irep.iium.edu.my/63485/ http://irep.iium.edu.my/63485/14/63485_Optimum%20Immobilization%20of%20%CE%B2-Glucosidase%20Enzyme_baru.pdf |
| id |
iium-63485 |
|---|---|
| recordtype |
eprints |
| spelling |
iium-634852018-06-29T03:25:37Z http://irep.iium.edu.my/63485/ Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness Maalim, Kauthar Yusuf Jameel, Ahmed Tariq Yusof, Faridah TP155 Chemical engineering TP248.13 Biotechnology The optimum conditions for immobilization of β-Glucosidase on multiwalled carbon nanotubes (MWCNTs) and Ca-alginate beads were studied. The effectiveness of the reaction of the immobilized enzyme with pnitrophenyl-β-D-glucopyranoside as substrate is evaluated. For the same substrate concentration the activity of enzyme immobilized in Ca-alginate beads was lower than that of immobilization on MWCNTs. The optimum immobilization conditions were found to be 17 mg MWCNT and 4 hr incubation time for MWCNTs while for calcium-alginate gel, it was found to be 3.5 wt% Na-alginate and 2 hr incubation time. The immobilization yield of the enzyme on MWCNTs was found to be 63.91% and that of Ca-alginate beads was 53.56%. Upon subsequent use, the residual activity of the enzyme on MWCNTs was higher than that of the Ca-alginate beads, which lost almost 50% of the activity at the third run. Both Ca-alginate and MWCNT immobilized enzyme were found to follow Michaelis-Menten kinetics and were well represented by Langmuir plot which was finally used to determine Michaelis-Menten constants. The values of Vmax and Km for MWCNTs are 4.7 μmol/mL.min and 0.833 mM respectively while for Ca-alginate the constants are 4.428 μmol/mL.min and 0.2587 mM respectively. Kulliyyah of Engineering International Islamic University Malaysia P.O. Box 10, 50728 Kuala Lumpur, Malaysia 2016-07-25 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/63485/14/63485_Optimum%20Immobilization%20of%20%CE%B2-Glucosidase%20Enzyme_baru.pdf Maalim, Kauthar Yusuf and Jameel, Ahmed Tariq and Yusof, Faridah (2016) Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness. In: International Conference Biotechnology Engineering, ICBioE ‘16 July 25-27, 2016, Kuala Lumpur, Malaysia, 25-27 July 2016, Kuala Lumpur, Malaysia. http://www.iium.edu.my/icbioe/2016/ |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP155 Chemical engineering TP248.13 Biotechnology |
| spellingShingle |
TP155 Chemical engineering TP248.13 Biotechnology Maalim, Kauthar Yusuf Jameel, Ahmed Tariq Yusof, Faridah Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness |
| description |
The optimum conditions for immobilization of β-Glucosidase on multiwalled carbon nanotubes (MWCNTs)
and Ca-alginate beads were studied. The effectiveness of the reaction of the immobilized enzyme with pnitrophenyl-β-D-glucopyranoside
as substrate is evaluated. For the same substrate concentration the activity
of enzyme immobilized in Ca-alginate beads was lower than that of immobilization on MWCNTs. The
optimum immobilization conditions were found to be 17 mg MWCNT and 4 hr incubation time for MWCNTs
while for calcium-alginate gel, it was found to be 3.5 wt% Na-alginate and 2 hr incubation time. The
immobilization yield of the enzyme on MWCNTs was found to be 63.91% and that of Ca-alginate beads was
53.56%. Upon subsequent use, the residual activity of the enzyme on MWCNTs was higher than that of the
Ca-alginate beads, which lost almost 50% of the activity at the third run. Both Ca-alginate and MWCNT
immobilized enzyme were found to follow Michaelis-Menten kinetics and were well represented by Langmuir
plot which was finally used to determine Michaelis-Menten constants. The values of Vmax and Km for
MWCNTs are 4.7 μmol/mL.min and 0.833 mM respectively while for Ca-alginate the constants are 4.428
μmol/mL.min and 0.2587 mM respectively. |
| format |
Conference or Workshop Item |
| author |
Maalim, Kauthar Yusuf Jameel, Ahmed Tariq Yusof, Faridah |
| author_facet |
Maalim, Kauthar Yusuf Jameel, Ahmed Tariq Yusof, Faridah |
| author_sort |
Maalim, Kauthar Yusuf |
| title |
Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness |
| title_short |
Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness |
| title_full |
Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness |
| title_fullStr |
Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness |
| title_full_unstemmed |
Optimum immobilization of β-Glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness |
| title_sort |
optimum immobilization of β-glucosidase enzyme in ca-alginate and acid functionalized-multiwalled carbon nanotubes and comparison of the catalyst effectiveness |
| publisher |
Kulliyyah of Engineering International Islamic University Malaysia P.O. Box 10, 50728 Kuala Lumpur, Malaysia |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/63485/ http://irep.iium.edu.my/63485/ http://irep.iium.edu.my/63485/14/63485_Optimum%20Immobilization%20of%20%CE%B2-Glucosidase%20Enzyme_baru.pdf |
| first_indexed |
2023-09-18T21:30:02Z |
| last_indexed |
2023-09-18T21:30:02Z |
| _version_ |
1777412449763328000 |