Antidiabetic characterization and mechanism of action of wogonin in rats

This research deals with the in vivo, in vitro, and in situ evaluations of antidiabetic effect of wogonin to understand its mechanism of action as an antdiabetic agent. Wogonin was isolated from the leaves extracts of T. indica. Its thirteen chemical analogues (including semisynthetic derivatives) w...

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Main Authors:	Ahmed, Qamar Uddin, Mat So'ad, Siti Zaiton, Bakhtiar, Muhammad Taher, Sarian, Murni Nazira, Alhassan, Alhassan Muhammad, Afrin, Mehnaz, Hasan, Md. Mahmudul (Stu), Latip, Jalifah
Format:	Monograph
Language:	English
Published:	2018
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/63544/ http://irep.iium.edu.my/63544/1/Profile%20of%20Final%20Report_FRGS13-089-0330_Dr.%20Qamar%20U.%20Ahmed.pdf

id	iium-63544
recordtype	eprints
spelling	iium-635442019-01-04T02:50:53Z http://irep.iium.edu.my/63544/ Antidiabetic characterization and mechanism of action of wogonin in rats Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Bakhtiar, Muhammad Taher Sarian, Murni Nazira Alhassan, Alhassan Muhammad Afrin, Mehnaz Hasan, Md. Mahmudul (Stu) Latip, Jalifah QD Chemistry This research deals with the in vivo, in vitro, and in situ evaluations of antidiabetic effect of wogonin to understand its mechanism of action as an antdiabetic agent. Wogonin was isolated from the leaves extracts of T. indica. Its thirteen chemical analogues (including semisynthetic derivatives) were also taken into account for structure activity relationship study and their structures were characterized by IR, UV, 1D and 2D NMR and MS spectral analysis. The results of animal study, cell culture antidiabetic (insulin secretion, adipogenesis, glucose uptake), and protein expression showed that the methyl ether group at position C-8 is responsible for wogonin’s antidiabetic property via β-cells of islet of langerhans’ recovery as well as through glucose uptake mechanism which indicated by up regulation of GLUT 4 and C/EBP-α. The mechanism could be enhanced by the addition of acetate group at C-5 and C-7. SAR study revealed that the total number and the configuration of hydroxyl groups play a vital role in regulating antidiabetic and antioxidant activities. Presence of C-2-C-3 double bond and C-4 ketonic group were found to be two essential structural features in the bioactivity of wogonin especially for antidiabetic property. For in situ experiments, the molecular docking study, quercetin showed the highest score among the chemical analogues of wogonin revealing the importance of hydroxyl groups. Results conclude that wogonin exerts antidiabetic effect through different mechanisms of action. In vivo study, it displayed antidiabetic effect through regenerating beta cells of pancreas, thereby found to increase the level of insulin in body. However, in vitro experiments, it showed effect like insulin and rosiglitazone by inducing adipogenesis and glucose uptake. It also showed α-glucosidase, dipeptyl peptidase-4 inhibitory effects, however, at high concentration. In vivo and in vitro results demonstrate that wogonin may exert its antidiabetic effect through multiple modes of action. SAR study showed that by removing its methoxy group and through addition of hydroxyl groups to the different positions of wogonin could increase its antidiabetic effect and decrease its toxicity. 2018-04-23 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/63544/1/Profile%20of%20Final%20Report_FRGS13-089-0330_Dr.%20Qamar%20U.%20Ahmed.pdf Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Bakhtiar, Muhammad Taher and Sarian, Murni Nazira and Alhassan, Alhassan Muhammad and Afrin, Mehnaz and Hasan, Md. Mahmudul (Stu) and Latip, Jalifah (2018) Antidiabetic characterization and mechanism of action of wogonin in rats. Research Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Bakhtiar, Muhammad Taher Sarian, Murni Nazira Alhassan, Alhassan Muhammad Afrin, Mehnaz Hasan, Md. Mahmudul (Stu) Latip, Jalifah Antidiabetic characterization and mechanism of action of wogonin in rats
description	This research deals with the in vivo, in vitro, and in situ evaluations of antidiabetic effect of wogonin to understand its mechanism of action as an antdiabetic agent. Wogonin was isolated from the leaves extracts of T. indica. Its thirteen chemical analogues (including semisynthetic derivatives) were also taken into account for structure activity relationship study and their structures were characterized by IR, UV, 1D and 2D NMR and MS spectral analysis. The results of animal study, cell culture antidiabetic (insulin secretion, adipogenesis, glucose uptake), and protein expression showed that the methyl ether group at position C-8 is responsible for wogonin’s antidiabetic property via β-cells of islet of langerhans’ recovery as well as through glucose uptake mechanism which indicated by up regulation of GLUT 4 and C/EBP-α. The mechanism could be enhanced by the addition of acetate group at C-5 and C-7. SAR study revealed that the total number and the configuration of hydroxyl groups play a vital role in regulating antidiabetic and antioxidant activities. Presence of C-2-C-3 double bond and C-4 ketonic group were found to be two essential structural features in the bioactivity of wogonin especially for antidiabetic property. For in situ experiments, the molecular docking study, quercetin showed the highest score among the chemical analogues of wogonin revealing the importance of hydroxyl groups. Results conclude that wogonin exerts antidiabetic effect through different mechanisms of action. In vivo study, it displayed antidiabetic effect through regenerating beta cells of pancreas, thereby found to increase the level of insulin in body. However, in vitro experiments, it showed effect like insulin and rosiglitazone by inducing adipogenesis and glucose uptake. It also showed α-glucosidase, dipeptyl peptidase-4 inhibitory effects, however, at high concentration. In vivo and in vitro results demonstrate that wogonin may exert its antidiabetic effect through multiple modes of action. SAR study showed that by removing its methoxy group and through addition of hydroxyl groups to the different positions of wogonin could increase its antidiabetic effect and decrease its toxicity.
format	Monograph
author	Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Bakhtiar, Muhammad Taher Sarian, Murni Nazira Alhassan, Alhassan Muhammad Afrin, Mehnaz Hasan, Md. Mahmudul (Stu) Latip, Jalifah
author_facet	Ahmed, Qamar Uddin Mat So'ad, Siti Zaiton Bakhtiar, Muhammad Taher Sarian, Murni Nazira Alhassan, Alhassan Muhammad Afrin, Mehnaz Hasan, Md. Mahmudul (Stu) Latip, Jalifah
author_sort	Ahmed, Qamar Uddin
title	Antidiabetic characterization and mechanism of action of wogonin in rats
title_short	Antidiabetic characterization and mechanism of action of wogonin in rats
title_full	Antidiabetic characterization and mechanism of action of wogonin in rats
title_fullStr	Antidiabetic characterization and mechanism of action of wogonin in rats
title_full_unstemmed	Antidiabetic characterization and mechanism of action of wogonin in rats
title_sort	antidiabetic characterization and mechanism of action of wogonin in rats
publishDate	2018
url	http://irep.iium.edu.my/63544/ http://irep.iium.edu.my/63544/1/Profile%20of%20Final%20Report_FRGS13-089-0330_Dr.%20Qamar%20U.%20Ahmed.pdf
first_indexed	2023-09-18T21:30:07Z
last_indexed	2023-09-18T21:30:07Z
_version_	1777412454849970176

Antidiabetic characterization and mechanism of action of wogonin in rats

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