Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
Background: Reduced cerebral blood flow is associated with aging, neurodegenerative disorders, and an increased formation of reactive oxygen species. This study was designed to assess the potential use of vitamin E as an antioxidant and neuroprotective agent using 2-vessel occlusion (2VO) rat animal...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Wolters Kluwer - Medknow
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/1/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection.pdf http://irep.iium.edu.my/63579/7/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection%20of%20Tocotrienols%20in%20Chronic%20Cerebral%20Hypoperfusion-Induced%20Neurodegeneration%20Rat%20Animal%20Model_SCOPUS.pdf |
| Summary: | Background: Reduced cerebral blood flow is associated with aging, neurodegenerative disorders, and an increased formation of reactive oxygen species. This study was designed to assess the potential use of vitamin E as an antioxidant and neuroprotective agent using 2-vessel occlusion (2VO) rat animal model. Materials and Methods: Twenty-four Sprague Dawley rats weighing 200–250 g were equally divided into the following three groups: SHAM control, 2VO, and 2VO+E (treated daily with vitamin E tocotrienol, 100 mg/kg, orally following 2VO). On the 8th week after 2VO surgery, rats were euthanized and the hippocampi were isolated with the estimation of viable neuronal cell count in the hippocampal CA-1 region. The isoprostane F2 (Iso-F2) levels were also measured in the brain homogenates to quantify the oxidative stress levels. Results: There was significantly higher neuronal cell death in the hippocampal CA-1 region and increased Iso-F2 levels in the 2VO group compared to the SHAM control group (P < 0.05). Conversely, no significant difference was observed with regard to the neuronal cell death and Iso-F2 levels in the 2VO+E group and the SHAM control group (P > 0.05). Conclusion: This study demonstrates the effectiveness of vitamin E tocotrienol as a neuroprotective and antioxidant agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats. |
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