Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model

Background: Reduced cerebral blood flow is associated with aging, neurodegenerative disorders, and an increased formation of reactive oxygen species. This study was designed to assess the potential use of vitamin E as an antioxidant and neuroprotective agent using 2-vessel occlusion (2VO) rat animal...

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Main Authors:	Mohamed, Wael Mohamed Yousef, Sayeed, Sayyada, Saxena, Anil Kumar, Syed Ahmad, Pakeer Oothuman
Format:	Article
Language:	English English
Published:	Wolters Kluwer - Medknow 2018
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/1/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection.pdf http://irep.iium.edu.my/63579/7/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection%20of%20Tocotrienols%20in%20Chronic%20Cerebral%20Hypoperfusion-Induced%20Neurodegeneration%20Rat%20Animal%20Model_SCOPUS.pdf

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spelling	iium-635792018-07-24T01:23:36Z http://irep.iium.edu.my/63579/ Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model Mohamed, Wael Mohamed Yousef Sayeed, Sayyada Saxena, Anil Kumar Syed Ahmad, Pakeer Oothuman R Medicine (General) Background: Reduced cerebral blood flow is associated with aging, neurodegenerative disorders, and an increased formation of reactive oxygen species. This study was designed to assess the potential use of vitamin E as an antioxidant and neuroprotective agent using 2-vessel occlusion (2VO) rat animal model. Materials and Methods: Twenty-four Sprague Dawley rats weighing 200–250 g were equally divided into the following three groups: SHAM control, 2VO, and 2VO+E (treated daily with vitamin E tocotrienol, 100 mg/kg, orally following 2VO). On the 8th week after 2VO surgery, rats were euthanized and the hippocampi were isolated with the estimation of viable neuronal cell count in the hippocampal CA-1 region. The isoprostane F2 (Iso-F2) levels were also measured in the brain homogenates to quantify the oxidative stress levels. Results: There was significantly higher neuronal cell death in the hippocampal CA-1 region and increased Iso-F2 levels in the 2VO group compared to the SHAM control group (P < 0.05). Conversely, no significant difference was observed with regard to the neuronal cell death and Iso-F2 levels in the 2VO+E group and the SHAM control group (P > 0.05). Conclusion: This study demonstrates the effectiveness of vitamin E tocotrienol as a neuroprotective and antioxidant agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats. Wolters Kluwer - Medknow 2018-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63579/1/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection.pdf application/pdf en http://irep.iium.edu.my/63579/7/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection%20of%20Tocotrienols%20in%20Chronic%20Cerebral%20Hypoperfusion-Induced%20Neurodegeneration%20Rat%20Animal%20Model_SCOPUS.pdf Mohamed, Wael Mohamed Yousef and Sayeed, Sayyada and Saxena, Anil Kumar and Syed Ahmad, Pakeer Oothuman (2018) Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model. International Journal of Nutrition, Pharmacology, Neurological Diseases, 8 (2). pp. 47-52. ISSN 2231-0738 E-ISSN 2231-2722 http://www.ijnpnd.com/article.asp?issn=2231-0738;year=2018;volume=8;issue=2;spage=47;epage=52;aulast=Mohamed;type=0 10.4103/ijnpnd.ijnpnd_17_18
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Mohamed, Wael Mohamed Yousef Sayeed, Sayyada Saxena, Anil Kumar Syed Ahmad, Pakeer Oothuman Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
description	Background: Reduced cerebral blood flow is associated with aging, neurodegenerative disorders, and an increased formation of reactive oxygen species. This study was designed to assess the potential use of vitamin E as an antioxidant and neuroprotective agent using 2-vessel occlusion (2VO) rat animal model. Materials and Methods: Twenty-four Sprague Dawley rats weighing 200–250 g were equally divided into the following three groups: SHAM control, 2VO, and 2VO+E (treated daily with vitamin E tocotrienol, 100 mg/kg, orally following 2VO). On the 8th week after 2VO surgery, rats were euthanized and the hippocampi were isolated with the estimation of viable neuronal cell count in the hippocampal CA-1 region. The isoprostane F2 (Iso-F2) levels were also measured in the brain homogenates to quantify the oxidative stress levels. Results: There was significantly higher neuronal cell death in the hippocampal CA-1 region and increased Iso-F2 levels in the 2VO group compared to the SHAM control group (P < 0.05). Conversely, no significant difference was observed with regard to the neuronal cell death and Iso-F2 levels in the 2VO+E group and the SHAM control group (P > 0.05). Conclusion: This study demonstrates the effectiveness of vitamin E tocotrienol as a neuroprotective and antioxidant agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.
format	Article
author	Mohamed, Wael Mohamed Yousef Sayeed, Sayyada Saxena, Anil Kumar Syed Ahmad, Pakeer Oothuman
author_facet	Mohamed, Wael Mohamed Yousef Sayeed, Sayyada Saxena, Anil Kumar Syed Ahmad, Pakeer Oothuman
author_sort	Mohamed, Wael Mohamed Yousef
title	Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
title_short	Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
title_full	Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
title_fullStr	Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
title_full_unstemmed	Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
title_sort	oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
publisher	Wolters Kluwer - Medknow
publishDate	2018
url	http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/1/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection.pdf http://irep.iium.edu.my/63579/7/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection%20of%20Tocotrienols%20in%20Chronic%20Cerebral%20Hypoperfusion-Induced%20Neurodegeneration%20Rat%20Animal%20Model_SCOPUS.pdf
first_indexed	2023-09-18T21:30:10Z
last_indexed	2023-09-18T21:30:10Z
_version_	1777412457837363200

Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model

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