Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems
Introduction: Quorum sensing controls a wide spectrum of processes and phenotypic behaviours including biofilm formation that helps in the survival and virulence of bacteria in hosts. Thus, anti-quorum sensing is suggested to combat bacterial infections. This study aimed to evaluate the anti-biofil...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kulliyyah of Medicine, International Islamic University Malaysia
2017
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| Online Access: | http://irep.iium.edu.my/63739/ http://irep.iium.edu.my/63739/ http://irep.iium.edu.my/63739/1/Farah_Abstract-Book-for-IMJM-final-v2-38.pdf |
| Summary: | Introduction: Quorum sensing controls a wide spectrum of processes and phenotypic behaviours including biofilm formation that helps in the survival and virulence of
bacteria in hosts. Thus, anti-quorum sensing is suggested to combat bacterial infections. This study aimed to evaluate the anti-biofilm forming and anti-quorum sensing activities which may contribute to the anti-bacterial effect of ethanol and aqueous extracts of Orthosiphon stamineus and Andrographis paniculata leaves and stems.
Materials and method: General biofilm assay protocol with modification was used for the biofilm assay to test the effect of different concentration (2 mg/ml and 3 mg/ml)
of extracts on the biofilm formation by Pseudomonas aeruginosa. Optical density of crystal violet/acetic acid solution was measured at 595 nm and the percentage of biofilm inhibition was calculated. In quorum quenching assay, visualization of reporter strain AI1-QQ.1 growth will be used for the detection of bio-molecules interfering with acyl homoserine lactone (AHL).
Results: Extracts with the highest ability to inhibit biofilm formation were ethanol extract of O.stamineus stem and A.paniculata leaves with 77.84% at extracts
concentration of 3 mg/ml. Aqueous extract of O.stamineus leaves showed the least potential with 13.41% inhibition. However, aqueous extract of A.paniculata showed zero inhibition at 3 mg/ml and two extracts (aqueous extract of O.stamineus and A.paniculata leaves) gave negative value at concentration of 3 mg/ml and 2 mg/ml,respectively. Quorum quenching assay has yet to be done.
Conclusion: It could be concluded that most of the extracts possess anti-biofilm
property. |
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