Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems
Introduction: Quorum sensing controls a wide spectrum of processes and phenotypic behaviours including biofilm formation that helps in the survival and virulence of bacteria in hosts. Thus, anti-quorum sensing is suggested to combat bacterial infections. This study aimed to evaluate the anti-biofil...
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Kulliyyah of Medicine, International Islamic University Malaysia
2017
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iium-637392018-05-21T07:45:34Z http://irep.iium.edu.my/63739/ Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems Yang Abd Talib, Farah Adiba Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen QR Microbiology QR355 Virology R Medicine (General) Introduction: Quorum sensing controls a wide spectrum of processes and phenotypic behaviours including biofilm formation that helps in the survival and virulence of bacteria in hosts. Thus, anti-quorum sensing is suggested to combat bacterial infections. This study aimed to evaluate the anti-biofilm forming and anti-quorum sensing activities which may contribute to the anti-bacterial effect of ethanol and aqueous extracts of Orthosiphon stamineus and Andrographis paniculata leaves and stems. Materials and method: General biofilm assay protocol with modification was used for the biofilm assay to test the effect of different concentration (2 mg/ml and 3 mg/ml) of extracts on the biofilm formation by Pseudomonas aeruginosa. Optical density of crystal violet/acetic acid solution was measured at 595 nm and the percentage of biofilm inhibition was calculated. In quorum quenching assay, visualization of reporter strain AI1-QQ.1 growth will be used for the detection of bio-molecules interfering with acyl homoserine lactone (AHL). Results: Extracts with the highest ability to inhibit biofilm formation were ethanol extract of O.stamineus stem and A.paniculata leaves with 77.84% at extracts concentration of 3 mg/ml. Aqueous extract of O.stamineus leaves showed the least potential with 13.41% inhibition. However, aqueous extract of A.paniculata showed zero inhibition at 3 mg/ml and two extracts (aqueous extract of O.stamineus and A.paniculata leaves) gave negative value at concentration of 3 mg/ml and 2 mg/ml,respectively. Quorum quenching assay has yet to be done. Conclusion: It could be concluded that most of the extracts possess anti-biofilm property. Kulliyyah of Medicine, International Islamic University Malaysia 2017-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63739/1/Farah_Abstract-Book-for-IMJM-final-v2-38.pdf Yang Abd Talib, Farah Adiba and Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen (2017) Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems. International Medical Journal Malaysia (IMJM), 16 (Supp. 1). ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2016%20No%201%20Supp%201/Abstract-Book-for-IMJM-final-v2-38.pdf |
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Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
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Online Access |
| language |
English |
| topic |
QR Microbiology QR355 Virology R Medicine (General) |
| spellingShingle |
QR Microbiology QR355 Virology R Medicine (General) Yang Abd Talib, Farah Adiba Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems |
| description |
Introduction: Quorum sensing controls a wide spectrum of processes and phenotypic behaviours including biofilm formation that helps in the survival and virulence of
bacteria in hosts. Thus, anti-quorum sensing is suggested to combat bacterial infections. This study aimed to evaluate the anti-biofilm forming and anti-quorum sensing activities which may contribute to the anti-bacterial effect of ethanol and aqueous extracts of Orthosiphon stamineus and Andrographis paniculata leaves and stems.
Materials and method: General biofilm assay protocol with modification was used for the biofilm assay to test the effect of different concentration (2 mg/ml and 3 mg/ml)
of extracts on the biofilm formation by Pseudomonas aeruginosa. Optical density of crystal violet/acetic acid solution was measured at 595 nm and the percentage of biofilm inhibition was calculated. In quorum quenching assay, visualization of reporter strain AI1-QQ.1 growth will be used for the detection of bio-molecules interfering with acyl homoserine lactone (AHL).
Results: Extracts with the highest ability to inhibit biofilm formation were ethanol extract of O.stamineus stem and A.paniculata leaves with 77.84% at extracts
concentration of 3 mg/ml. Aqueous extract of O.stamineus leaves showed the least potential with 13.41% inhibition. However, aqueous extract of A.paniculata showed zero inhibition at 3 mg/ml and two extracts (aqueous extract of O.stamineus and A.paniculata leaves) gave negative value at concentration of 3 mg/ml and 2 mg/ml,respectively. Quorum quenching assay has yet to be done.
Conclusion: It could be concluded that most of the extracts possess anti-biofilm
property. |
| format |
Article |
| author |
Yang Abd Talib, Farah Adiba Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen |
| author_facet |
Yang Abd Talib, Farah Adiba Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen |
| author_sort |
Yang Abd Talib, Farah Adiba |
| title |
Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems |
| title_short |
Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems |
| title_full |
Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems |
| title_fullStr |
Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems |
| title_full_unstemmed |
Anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems |
| title_sort |
anti-biofilm and anti-quorum sensing properties of ethanol and aqueous extracts of orthosiphon stamineus and andrographis paniculata leaves and stems |
| publisher |
Kulliyyah of Medicine, International Islamic University Malaysia |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/63739/ http://irep.iium.edu.my/63739/ http://irep.iium.edu.my/63739/1/Farah_Abstract-Book-for-IMJM-final-v2-38.pdf |
| first_indexed |
2023-09-18T21:30:23Z |
| last_indexed |
2023-09-18T21:30:23Z |
| _version_ |
1777412471072489472 |