Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene

Epigenetic DNA methylation and Schizophrenia The evidence of Disrupted in Schizophrenia 1 (DISC1) gene role in schizophrenia came through functional and animal studies. However the exact genetic basis is yet established. As meta-analyses findings have denied DISC1 variants as the genetic basis...

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Main Authors:	A.Talib, Norlelawati, Ku Zaifah, Norsidah, Abdullah, Kartini, Abd. Rahim, Nour El Huda
Format:	Monograph
Language:	English
Published:	2018
Subjects:	RM Therapeutics. Pharmacology RZ400 Mental healing
Online Access:	http://irep.iium.edu.my/63787/ http://irep.iium.edu.my/63787/1/PROJECT%20REPORT%20PROFILE.pdf

id	iium-63787
recordtype	eprints
spelling	iium-637872019-01-11T03:03:00Z http://irep.iium.edu.my/63787/ Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene A.Talib, Norlelawati Ku Zaifah, Norsidah Abdullah, Kartini Abd. Rahim, Nour El Huda RM Therapeutics. Pharmacology RZ400 Mental healing Epigenetic DNA methylation and Schizophrenia The evidence of Disrupted in Schizophrenia 1 (DISC1) gene role in schizophrenia came through functional and animal studies. However the exact genetic basis is yet established. As meta-analyses findings have denied DISC1 variants as the genetic basis, emphasis is now shifted to genetic defect such as copy number and epigenetic which have not been well explored. The project explored the epigenetic regulation of DISC1 gene and other neurodevelopmental and neurotransmitter genes that were strongly advocated as candidate genes of schizophrenia. A comparative cross sectional study consisted of 110 schizophrenia and 122 healthy control were recruited. Method for DNA methylation quantitation using high resolution melting (HRM) and MethylLight assays were established and used to quantify the methylation percentage. The optimized HRM assay for DISC1clearly demonstrated the serial methylated percentage differentiation of 0%, 25%, 50%, 75% and 100% accordingly. However, we found DISC1 methylation has no relationship with schizophrenia (p >0.05). With similar approach, COMT and RELN DNA methylation were investigated. We found, a significantly higher level of methylation of the RELN and COMT promoter in patients with schizophrenia compared to controls (p=0.005) and reduced RELN but not COMT expression in the methylated group as compared to the non-methylated group. The findings suggest possibility of DNA methylation of RELN and COMT gene effects in the pathogenesis of schizophrenia. 2018-05 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/63787/1/PROJECT%20REPORT%20PROFILE.pdf A.Talib, Norlelawati and Ku Zaifah, Norsidah and Abdullah, Kartini and Abd. Rahim, Nour El Huda (2018) Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene. Project Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology RZ400 Mental healing
spellingShingle	RM Therapeutics. Pharmacology RZ400 Mental healing A.Talib, Norlelawati Ku Zaifah, Norsidah Abdullah, Kartini Abd. Rahim, Nour El Huda Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
description	Epigenetic DNA methylation and Schizophrenia The evidence of Disrupted in Schizophrenia 1 (DISC1) gene role in schizophrenia came through functional and animal studies. However the exact genetic basis is yet established. As meta-analyses findings have denied DISC1 variants as the genetic basis, emphasis is now shifted to genetic defect such as copy number and epigenetic which have not been well explored. The project explored the epigenetic regulation of DISC1 gene and other neurodevelopmental and neurotransmitter genes that were strongly advocated as candidate genes of schizophrenia. A comparative cross sectional study consisted of 110 schizophrenia and 122 healthy control were recruited. Method for DNA methylation quantitation using high resolution melting (HRM) and MethylLight assays were established and used to quantify the methylation percentage. The optimized HRM assay for DISC1clearly demonstrated the serial methylated percentage differentiation of 0%, 25%, 50%, 75% and 100% accordingly. However, we found DISC1 methylation has no relationship with schizophrenia (p >0.05). With similar approach, COMT and RELN DNA methylation were investigated. We found, a significantly higher level of methylation of the RELN and COMT promoter in patients with schizophrenia compared to controls (p=0.005) and reduced RELN but not COMT expression in the methylated group as compared to the non-methylated group. The findings suggest possibility of DNA methylation of RELN and COMT gene effects in the pathogenesis of schizophrenia.
format	Monograph
author	A.Talib, Norlelawati Ku Zaifah, Norsidah Abdullah, Kartini Abd. Rahim, Nour El Huda
author_facet	A.Talib, Norlelawati Ku Zaifah, Norsidah Abdullah, Kartini Abd. Rahim, Nour El Huda
author_sort	A.Talib, Norlelawati
title	Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_short	Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_full	Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_fullStr	Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_full_unstemmed	Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_sort	epigenetics of schizophrenia: a focus on the dna methylation mechanism and expression of disc1 gene
publishDate	2018
url	http://irep.iium.edu.my/63787/ http://irep.iium.edu.my/63787/1/PROJECT%20REPORT%20PROFILE.pdf
first_indexed	2023-09-18T21:30:28Z
last_indexed	2023-09-18T21:30:28Z
_version_	1777412476791422976

Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene

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