Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employ...

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Main Authors: Yusan, Sabriye, M. Rahman, Mokhlesur, Mohamad, Nasir A.N., ,, Tengku M. Arrif, Abdul Latif, Ahmad Zubaidi, Md Aris, Mohd Aznan, Wan Nik, Wan Sani
Format: Article
Language:English
English
English
Published: Hindawi 2018
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http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf
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spelling iium-637882018-07-30T02:23:14Z http://irep.iium.edu.my/63788/ Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RZ Other systems of medicine A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibrationplotisobtainedoverawideconcentrationrangeofglucosefrom1×10−5 to2×10−3M.Underoptimalconditions,the biosensor exhibits high sensitivity (0.34µA·mM−1), low detection limit (1×10−4M), high affinity to glucose (Km�0.02mM), and also good reproducibility (R.S.D. 2.8%, n � 10) and a stability of about ten days when stored dry at +4°C. Besides, the effectsofpHvalue,scanrate,mediatoreffectsontheglucosecurrent,andelectroactiveinterferenceofthebiosensorwerealso discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility. Hindawi 2018-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf application/pdf en http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf Yusan, Sabriye and M. Rahman, Mokhlesur and Mohamad, Nasir A.N. and ,, Tengku M. Arrif and Abdul Latif, Ahmad Zubaidi and Md Aris, Mohd Aznan and Wan Nik, Wan Sani (2018) Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite. Journal of Analytical Methods in Chemistry, 2018. pp. 1-8. ISSN 2090-8865 E-ISSN 2090-8873 https://doi.org/10.1155/2018/2687341 10.1155/2018/2687341
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
topic R Medicine (General)
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
RZ Other systems of medicine
spellingShingle R Medicine (General)
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
RZ Other systems of medicine
Yusan, Sabriye
M. Rahman, Mokhlesur
Mohamad, Nasir A.N.
,, Tengku M. Arrif
Abdul Latif, Ahmad Zubaidi
Md Aris, Mohd Aznan
Wan Nik, Wan Sani
Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
description A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibrationplotisobtainedoverawideconcentrationrangeofglucosefrom1×10−5 to2×10−3M.Underoptimalconditions,the biosensor exhibits high sensitivity (0.34µA·mM−1), low detection limit (1×10−4M), high affinity to glucose (Km�0.02mM), and also good reproducibility (R.S.D. 2.8%, n � 10) and a stability of about ten days when stored dry at +4°C. Besides, the effectsofpHvalue,scanrate,mediatoreffectsontheglucosecurrent,andelectroactiveinterferenceofthebiosensorwerealso discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.
format Article
author Yusan, Sabriye
M. Rahman, Mokhlesur
Mohamad, Nasir A.N.
,, Tengku M. Arrif
Abdul Latif, Ahmad Zubaidi
Md Aris, Mohd Aznan
Wan Nik, Wan Sani
author_facet Yusan, Sabriye
M. Rahman, Mokhlesur
Mohamad, Nasir A.N.
,, Tengku M. Arrif
Abdul Latif, Ahmad Zubaidi
Md Aris, Mohd Aznan
Wan Nik, Wan Sani
author_sort Yusan, Sabriye
title Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_short Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_full Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_fullStr Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_full_unstemmed Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
title_sort development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the se-mcm-41 mesoporous composite
publisher Hindawi
publishDate 2018
url http://irep.iium.edu.my/63788/
http://irep.iium.edu.my/63788/
http://irep.iium.edu.my/63788/
http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf
http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf
http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf
first_indexed 2023-09-18T21:30:28Z
last_indexed 2023-09-18T21:30:28Z
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