The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study

Nigella sativa (NS) or also known as black seeds is one of the traditional herbs that were identified to have anticancer properties due to the presence of an active compound, thymoquinone. In this study, the cytotoxic effect of the aqueous extracts of NS were tested on the breast cancer cell line, M...

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Main Authors:	Bumidin, Muhammad Solehuddin, Johari, Farah Azurin, Risan, Nurul Fikri, Mohd Nasir, Mohd Hamzah
Format:	Article
Language:	English
Published:	Zibeline International Publisher 2018
Subjects:	QH301 Biology
Online Access:	http://irep.iium.edu.my/63921/ http://irep.iium.edu.my/63921/ http://irep.iium.edu.my/63921/ http://irep.iium.edu.my/63921/1/1gws2018-13-17.pdf

id	iium-63921
recordtype	eprints
spelling	iium-639212018-05-31T02:03:01Z http://irep.iium.edu.my/63921/ The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study Bumidin, Muhammad Solehuddin Johari, Farah Azurin Risan, Nurul Fikri Mohd Nasir, Mohd Hamzah QH301 Biology Nigella sativa (NS) or also known as black seeds is one of the traditional herbs that were identified to have anticancer properties due to the presence of an active compound, thymoquinone. In this study, the cytotoxic effect of the aqueous extracts of NS were tested on the breast cancer cell line, MCF-7. Aqueous extraction of NS using cold water producing 3.8% of yield. The cytotoxic effect of aqueous extracts of NS were tested after incubation at 5 hours, 24 hours and 48 hours by using a modified MTT colorimetric assay. Interestingly, the IC50 of aqueous extracts of NS on MCF-7 was at 11.5 µg/ml. Additionally, aqueous extracts of NS at the concentration beyond 11.5 µg/ml causing the swelling of MCF-7 and leads to cell burst. In conclusion, aqueous extracts of NS have been proved to have cytotoxic effect on MCF-7. Zibeline International Publisher 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63921/1/1gws2018-13-17.pdf Bumidin, Muhammad Solehuddin and Johari, Farah Azurin and Risan, Nurul Fikri and Mohd Nasir, Mohd Hamzah (2018) The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study. Science Heritage Journal \| Galeri Warisan Sains (GWS), 2 (1). pp. 13-17. ISSN 2521-0858 E-ISSN 2521-0866 https://www.zibelinepub.com/index.php/2018/05/21/the-effect-of-aqueous-extracts-of-nigella-sativa-on-breast-cancer-cell-line-mcf-7-an-in-vitro-study/ 10.26480/gws.01.2018.13.17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QH301 Biology
spellingShingle	QH301 Biology Bumidin, Muhammad Solehuddin Johari, Farah Azurin Risan, Nurul Fikri Mohd Nasir, Mohd Hamzah The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study
description	Nigella sativa (NS) or also known as black seeds is one of the traditional herbs that were identified to have anticancer properties due to the presence of an active compound, thymoquinone. In this study, the cytotoxic effect of the aqueous extracts of NS were tested on the breast cancer cell line, MCF-7. Aqueous extraction of NS using cold water producing 3.8% of yield. The cytotoxic effect of aqueous extracts of NS were tested after incubation at 5 hours, 24 hours and 48 hours by using a modified MTT colorimetric assay. Interestingly, the IC50 of aqueous extracts of NS on MCF-7 was at 11.5 µg/ml. Additionally, aqueous extracts of NS at the concentration beyond 11.5 µg/ml causing the swelling of MCF-7 and leads to cell burst. In conclusion, aqueous extracts of NS have been proved to have cytotoxic effect on MCF-7.
format	Article
author	Bumidin, Muhammad Solehuddin Johari, Farah Azurin Risan, Nurul Fikri Mohd Nasir, Mohd Hamzah
author_facet	Bumidin, Muhammad Solehuddin Johari, Farah Azurin Risan, Nurul Fikri Mohd Nasir, Mohd Hamzah
author_sort	Bumidin, Muhammad Solehuddin
title	The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study
title_short	The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study
title_full	The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study
title_fullStr	The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study
title_full_unstemmed	The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study
title_sort	effect of aqueous extracts of nigella sativa on breast cancer cell line mcf-7: an in vitro study
publisher	Zibeline International Publisher
publishDate	2018
url	http://irep.iium.edu.my/63921/ http://irep.iium.edu.my/63921/ http://irep.iium.edu.my/63921/ http://irep.iium.edu.my/63921/1/1gws2018-13-17.pdf
first_indexed	2023-09-18T21:30:40Z
last_indexed	2023-09-18T21:30:40Z
_version_	1777412488778743808

The effect of aqueous extracts of Nigella sativa on breast cancer cell line MCF-7: An in vitro study

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