Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells

Objective: The aim of this study was to investigate in vitro anti-inflammatory activity of tinocrisposide using lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophage cells. Tinocrisposide is a furano diterpene glycoside that was isolated in our previous study from Tinospora crispa. Methods: An...

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Main Authors:	Adnan, Adek Zamrud, Bakhtiar, Muhammad Taher, Afriani, Tika, ,, Annisa Fauzana, Roesma, Dewi Imelda, Putra, Andani Eka
Format:	Article
Language:	English English
Published:	Asian Journal of Pharmaceutical and Clinical Research 2018
Subjects:	QP Physiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/64032/ http://irep.iium.edu.my/64032/ http://irep.iium.edu.my/64032/ http://irep.iium.edu.my/64032/1/64032_ANTI-INFLAMMATORY%20ACTIVITY%20OF%20TINOCRISPOSIDE_article.pdf http://irep.iium.edu.my/64032/2/64032_ANTI-INFLAMMATORY%20ACTIVITY%20OF%20TINOCRISPOSIDE_scopus.pdf

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spelling	iium-640322018-06-01T05:56:10Z http://irep.iium.edu.my/64032/ Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells Adnan, Adek Zamrud Bakhtiar, Muhammad Taher Afriani, Tika ,, Annisa Fauzana Roesma, Dewi Imelda Putra, Andani Eka QP Physiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Objective: The aim of this study was to investigate in vitro anti-inflammatory activity of tinocrisposide using lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophage cells. Tinocrisposide is a furano diterpene glycoside that was isolated in our previous study from Tinospora crispa. Methods: Anti-inflammatory effect was quantified spectrometrically using Griess method by measuring nitric oxide (NO) production after the addition of Griess reagent. Results: The sample concentrations of 1, 5, 25, 50, and 100 µM and 100 µM of dexamethasone (positive control) have been tested against the LPSstimulated RAW 264.7 cells, and the results showed NO level production of 39.23, 34.00, 28.9, 20.25, 16.3, and 13.68 µM, respectively, and the inhibition level of 22.67, 33.00, 43.03, 60.10, 68.00, and 73%, respectively. Conclusions: From the study, it could be concluded that tinocrisposide was able to inhibit the formation of NO in the LPS-stimulated RAW 264.7 cells in concentration activity-dependent manner, with half-maximal inhibition concentration 46.92 µM. It can be developed as anti-inflammatory candidate drug because NO is a reactive nitrogen species which is produced by NO synthase. The production of NO has been established as a mediator in inflammatory diseases. Asian Journal of Pharmaceutical and Clinical Research 2018-04 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/64032/1/64032_ANTI-INFLAMMATORY%20ACTIVITY%20OF%20TINOCRISPOSIDE_article.pdf application/pdf en http://irep.iium.edu.my/64032/2/64032_ANTI-INFLAMMATORY%20ACTIVITY%20OF%20TINOCRISPOSIDE_scopus.pdf Adnan, Adek Zamrud and Bakhtiar, Muhammad Taher and Afriani, Tika and ,, Annisa Fauzana and Roesma, Dewi Imelda and Putra, Andani Eka (2018) Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells. Asian Journal of Pharmaceutical and Clinical Research, 11 (4). pp. 149-153. ISSN 0974-2441 E-ISSN 2455-3891 https://innovareacademics.in/journals/index.php/ajpcr/article/view/23739/14349 10.22159/ajpcr.2018.v11i4.23739
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QP Physiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	QP Physiology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Adnan, Adek Zamrud Bakhtiar, Muhammad Taher Afriani, Tika ,, Annisa Fauzana Roesma, Dewi Imelda Putra, Andani Eka Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells
description	Objective: The aim of this study was to investigate in vitro anti-inflammatory activity of tinocrisposide using lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophage cells. Tinocrisposide is a furano diterpene glycoside that was isolated in our previous study from Tinospora crispa. Methods: Anti-inflammatory effect was quantified spectrometrically using Griess method by measuring nitric oxide (NO) production after the addition of Griess reagent. Results: The sample concentrations of 1, 5, 25, 50, and 100 µM and 100 µM of dexamethasone (positive control) have been tested against the LPSstimulated RAW 264.7 cells, and the results showed NO level production of 39.23, 34.00, 28.9, 20.25, 16.3, and 13.68 µM, respectively, and the inhibition level of 22.67, 33.00, 43.03, 60.10, 68.00, and 73%, respectively. Conclusions: From the study, it could be concluded that tinocrisposide was able to inhibit the formation of NO in the LPS-stimulated RAW 264.7 cells in concentration activity-dependent manner, with half-maximal inhibition concentration 46.92 µM. It can be developed as anti-inflammatory candidate drug because NO is a reactive nitrogen species which is produced by NO synthase. The production of NO has been established as a mediator in inflammatory diseases.
format	Article
author	Adnan, Adek Zamrud Bakhtiar, Muhammad Taher Afriani, Tika ,, Annisa Fauzana Roesma, Dewi Imelda Putra, Andani Eka
author_facet	Adnan, Adek Zamrud Bakhtiar, Muhammad Taher Afriani, Tika ,, Annisa Fauzana Roesma, Dewi Imelda Putra, Andani Eka
author_sort	Adnan, Adek Zamrud
title	Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells
title_short	Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells
title_full	Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells
title_fullStr	Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells
title_full_unstemmed	Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells
title_sort	anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells
publisher	Asian Journal of Pharmaceutical and Clinical Research
publishDate	2018
url	http://irep.iium.edu.my/64032/ http://irep.iium.edu.my/64032/ http://irep.iium.edu.my/64032/ http://irep.iium.edu.my/64032/1/64032_ANTI-INFLAMMATORY%20ACTIVITY%20OF%20TINOCRISPOSIDE_article.pdf http://irep.iium.edu.my/64032/2/64032_ANTI-INFLAMMATORY%20ACTIVITY%20OF%20TINOCRISPOSIDE_scopus.pdf
first_indexed	2023-09-18T21:30:48Z
last_indexed	2023-09-18T21:30:48Z
_version_	1777412497996775424

Anti-inflammatory activity of tinocrisposide by inhibiting nitric oxide production in lipopolysaccharides-stimulated raw 264.7 cells

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