Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes

INTRODUCTION: One of the challenging aspects of orthopaedic surgery with regards to the long term functional outcome is intra-articular fixation. A variety of indications among others include intra-articular fracture, osteotomy, ligament reconstruction, meniscal and cartilage repair. The great pot...

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Main Authors:	Balakrishnan, Theenesh, Zulkifly, Ahmad Hafiz, Sha'ban, Munirah, Mohd Jan, Nurul Hafiza, Ibrahim, Mohd Zulfadzli, Md Nazir, Noorhidayah
Format:	Article
Language:	English
Published:	Malaysian Orthopaedic Association (MOA) 2017
Subjects:	RD701 Orthopedics
Online Access:	http://irep.iium.edu.my/64067/ http://irep.iium.edu.my/64067/ http://irep.iium.edu.my/64067/1/FR-04-Theenesh-Balakrishnan.pdf

id	iium-64067
recordtype	eprints
spelling	iium-640672018-07-02T08:31:07Z http://irep.iium.edu.my/64067/ Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes Balakrishnan, Theenesh Zulkifly, Ahmad Hafiz Sha'ban, Munirah Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Md Nazir, Noorhidayah RD701 Orthopedics INTRODUCTION: One of the challenging aspects of orthopaedic surgery with regards to the long term functional outcome is intra-articular fixation. A variety of indications among others include intra-articular fracture, osteotomy, ligament reconstruction, meniscal and cartilage repair. The great potential of biodegradable polymers in the above-mentioned cases are gradually being recognized. MATERIALS & METHODS: Objectives : 1) To compare biocompatibility and biodegradability of polymer (PLGA+fibrin) with PLGA for intra-articular screw fixation 2) to study the imaging (microradiograph), histology and histomorphometry features METHODS: We used fabricated PLGA scaffolds in combination with autologous fibrin for an in-vivo prospective research. Total of 9 New Zealand White Rabbits (NZWR) were operated and the scaffolds were placed at both medial and lateral femoral condyles of the right knee and those with fibrin at the left knee. Post implantation, evaluation was done at 6,12 and 24 weeks (3 NZWR in each group). For microradiological assessment, micro CT (Skyscan 1176) was used. Specimens were stained with Masson-Goldner trichrome method for histology and histomorphometry evaluation RESULTS: The combination of PLGA and fibrin has better biocompatibility, showed faster biodegradation and more quantitative integration of osseous tissues. (refer table 1) DISCUSSION: A major intent of biodegradable implants is complete osseous replacement at the former implant site. The duration of the degradation of the polymer is an important factor in influencing new bone formation. The first reaction at the implant site consists of bone resorption stimulated by the byproducts released during the degradation, then followed by fibrous tissue formation and completed by gradual increase in trabecular bone volume plus osteoid surface fraction, all of which can be demonstrated by computed tomography (CT) scans and histomorphometry slides. TABLE 1 CONCLUSION: Biodegradable polymer PLGA with incorporation of fibrin results in superior outcome compared to usage of other current biodegradable polymers. Malaysian Orthopaedic Association (MOA) 2017-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64067/1/FR-04-Theenesh-Balakrishnan.pdf Balakrishnan, Theenesh and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah and Mohd Jan, Nurul Hafiza and Ibrahim, Mohd Zulfadzli and Md Nazir, Noorhidayah (2017) Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes. Malaysian Orthopaedic Journal, 11 (Supplement A). FR 04-2397/64067. ISSN 1985-2533 E-ISSN 2232-111X http://www.morthoj.org/supplements/moa/FR-04-Theenesh-Balakrishnan.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RD701 Orthopedics
spellingShingle	RD701 Orthopedics Balakrishnan, Theenesh Zulkifly, Ahmad Hafiz Sha'ban, Munirah Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Md Nazir, Noorhidayah Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes
description	INTRODUCTION: One of the challenging aspects of orthopaedic surgery with regards to the long term functional outcome is intra-articular fixation. A variety of indications among others include intra-articular fracture, osteotomy, ligament reconstruction, meniscal and cartilage repair. The great potential of biodegradable polymers in the above-mentioned cases are gradually being recognized. MATERIALS & METHODS: Objectives : 1) To compare biocompatibility and biodegradability of polymer (PLGA+fibrin) with PLGA for intra-articular screw fixation 2) to study the imaging (microradiograph), histology and histomorphometry features METHODS: We used fabricated PLGA scaffolds in combination with autologous fibrin for an in-vivo prospective research. Total of 9 New Zealand White Rabbits (NZWR) were operated and the scaffolds were placed at both medial and lateral femoral condyles of the right knee and those with fibrin at the left knee. Post implantation, evaluation was done at 6,12 and 24 weeks (3 NZWR in each group). For microradiological assessment, micro CT (Skyscan 1176) was used. Specimens were stained with Masson-Goldner trichrome method for histology and histomorphometry evaluation RESULTS: The combination of PLGA and fibrin has better biocompatibility, showed faster biodegradation and more quantitative integration of osseous tissues. (refer table 1) DISCUSSION: A major intent of biodegradable implants is complete osseous replacement at the former implant site. The duration of the degradation of the polymer is an important factor in influencing new bone formation. The first reaction at the implant site consists of bone resorption stimulated by the byproducts released during the degradation, then followed by fibrous tissue formation and completed by gradual increase in trabecular bone volume plus osteoid surface fraction, all of which can be demonstrated by computed tomography (CT) scans and histomorphometry slides. TABLE 1 CONCLUSION: Biodegradable polymer PLGA with incorporation of fibrin results in superior outcome compared to usage of other current biodegradable polymers.
format	Article
author	Balakrishnan, Theenesh Zulkifly, Ahmad Hafiz Sha'ban, Munirah Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Md Nazir, Noorhidayah
author_facet	Balakrishnan, Theenesh Zulkifly, Ahmad Hafiz Sha'ban, Munirah Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Md Nazir, Noorhidayah
author_sort	Balakrishnan, Theenesh
title	Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes
title_short	Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes
title_full	Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes
title_fullStr	Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes
title_full_unstemmed	Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes
title_sort	comparison of biocompatibility and biodegradability of poly(lactic-co-glycolic acid) (plga) combined with autologous fibrin versus plga for intra-articular screw fixation; an in-vivo study with new zealand white rabbits–the microradiograph, histology and histomorphometry outcomes
publisher	Malaysian Orthopaedic Association (MOA)
publishDate	2017
url	http://irep.iium.edu.my/64067/ http://irep.iium.edu.my/64067/ http://irep.iium.edu.my/64067/1/FR-04-Theenesh-Balakrishnan.pdf
first_indexed	2023-09-18T21:30:52Z
last_indexed	2023-09-18T21:30:52Z
_version_	1777412501497970688

Comparison of biocompatibility and biodegradability of Poly(Lactic-Co-Glycolic acid) (PLGA) combined with autologous fibrin versus PLGA for intra-articular screw fixation; an in-vivo study with New Zealand white rabbits–The microradiograph, histology and histomorphometry outcomes

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